Non-induction of radioadaptive response in zebrafish embryos by neutrons

Ng, C. Y. P.; Kong, Eva Yi; Kobayashi, Adam; Suya, Noriyoshi; Uchihori, Y.; Cheng, Shuk Han; Konishi, Teruaki; Yu, K.N.

doi:10.1093/jrr/rrv089

Cited by 7 publications

(10 citation statements)

References 65 publications

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“…zebrafish embryos) were irradiated using Neutron exposure Accelerator System for Biological Effect Experiments (NASBEE) facility at the National Institute of Radiological Sciences (NIRS), Chiba, Japan [6]. Furthermore, similar discrepancies were also present in terms of RAR, in which the failure of neutrons to induce RAR in zebrafish embryos and human lymphocytes has been previously reported [7,8] and in contrast, the induction of RAR in Chinese hamster V79 cells was reported in the previous work of Marples and Shov [9]. …”

Section: Introductionmentioning

confidence: 90%

Monte Carlo studies on neutron interactions in radiobiological experiments

et al. 2017

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Monte Carlo method was used to study the characteristics of neutron interactions with cells underneath a water medium layer with varying thickness. The following results were obtained. (1) The fractions of neutron interaction with 1H, 12C, 14N and 16O nuclei in the cell layer were studied. The fraction with 1H increased with increasing medium thickness, while decreased for 12C, 14N and 16O nuclei. The bulges in the interaction fractions with 12C, 14N and 16O nuclei were explained by the resonance spikes in the interaction cross-section data. The interaction fraction decreased in the order: 1H > 16O > 12C > 14N. (2) In general, as the medium thickness increased, the number of “interacting neutrons” which exited the medium and then further interacted with the cell layer increased. (3) The area under the angular distributions for “interacting neutrons” decreased with increasing incident neutron energy. Such results would be useful for deciphering the reasons behind discrepancies among existing results in the literature.

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Section: Introductionmentioning

confidence: 90%

Monte Carlo studies on neutron interactions in radiobiological experiments

et al. 2017

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“…[ 5 ] and Ng et al . [ 6 ] demonstrated that neutrons failed to induce a RAR in human lymphocytes and zebrafish embryos, respectively. In contrast, Marples and Shov [ 7 ] revealed a neutron-induced RAR in Chinese hamster V79 cells.…”

Section: Introductionmentioning

confidence: 99%

A calibration method for realistic neutron dosimetry in radiobiological experiments assisted by MCNP simulation

Beni

Krstić

Nikezić

et al. 2016

Journal of Radiation Research

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Many studies on biological effects of neutrons involve dose responses of neutrons, which rely on accurately determined absorbed doses in the irradiated cells or living organisms. Absorbed doses are difficult to measure, and are commonly surrogated with doses measured using separate detectors. The present work describes the determination of doses absorbed in the cell layer underneath a medium column (DA) and the doses absorbed in an ionization chamber (DE) from neutrons through computer simulations using the MCNP-5 code, and the subsequent determination of the conversion coefficients R (= DA/DE). It was found that R in general decreased with increase in the medium thickness, which was due to elastic and inelastic scattering. For 2-MeV neutrons, conspicuous bulges in R values were observed at medium thicknesses of about 500, 1500, 2500 and 4000 μm, and these were attributed to carbon, oxygen and nitrogen nuclei, and were reflections of spikes in neutron interaction cross sections with these nuclei. For 0.1-MeV neutrons, no conspicuous bulges in R were observed (except one at ~2000 μm that was due to photon interactions), which was explained by the absence of prominent spikes in the interaction cross-sections with these nuclei for neutron energies <0.1 MeV. The ratio R could be increased by ~50% for small medium thickness if the incident neutron energy was reduced from 2 MeV to 0.1 MeV. As such, the absorbed doses in cells (DA) would vary with the incident neutron energies, even when the absorbed doses shown on the detector were the same.

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“…Apoptosis was also used as the biological endpoint in many previous studies on radiobiological effects in zebrafish embryos (see e.g., [27,33,35,36,37,38,42,43,45,46,47,48,49]. As reviewed in [39], the extra apoptotic events developed in irradiated zebrafish embryos likely reflected genomic instability induced in the irradiated cells (at 5 hpf), since these irradiated cells and their earlier progeny, which were unstable and mutation prone, had appeared to be healthy and were able to survive many generations before the apoptosis pathways were initiated (at 20, 24, or 30 hpf).…”

Section: Discussionmentioning

confidence: 99%

Effect of Photon Hormesis on Dose Responses to Alpha Particles in Zebrafish Embryos

Cheng

2017

IJMS

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Photon hormesis refers to the phenomenon where the biological effect of ionizing radiation with a high linear energy transfer (LET) value is diminished by photons with a low LET value. The present paper studied the effect of photon hormesis from X-rays on dose responses to alpha particles using embryos of the zebrafish (Danio rerio) as the in vivo vertebrate model. The toxicity of these ionizing radiations in the zebrafish embryos was assessed using the apoptotic counts at 20, 24, or 30 h post fertilization (hpf) revealed through acridine orange (AO) staining. For alpha-particle doses ≥ 4.4 mGy, the additional X-ray dose of 10 mGy significantly reduced the number of apoptotic cells at 24 hpf, which proved the presence of photon hormesis. Smaller alpha-particle doses might not have inflicted sufficient aggregate damages to trigger photon hormesis. The time gap T between the X-ray (10 mGy) and alpha-particle (4.4 mGy) exposures was also studied. Photon hormesis was present when T ≤ 30 min, but was absent when T = 60 min, at which time repair of damage induced by alpha particles would have completed to prevent their interactions with those induced by X-rays. Finally, the drop in the apoptotic counts at 24 hpf due to photon hormesis was explained by bringing the apoptotic events earlier to 20 hpf, which strongly supported the removal of aberrant cells through apoptosis as an underlying mechanism for photon hormesis.

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Non-induction of radioadaptive response in zebrafish embryos by neutrons

Cited by 7 publications

References 65 publications

Monte Carlo studies on neutron interactions in radiobiological experiments

Monte Carlo studies on neutron interactions in radiobiological experiments

A calibration method for realistic neutron dosimetry in radiobiological experiments assisted by MCNP simulation

Effect of Photon Hormesis on Dose Responses to Alpha Particles in Zebrafish Embryos

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