Non-immunoglobulin scaffolds: a focus on their targets

Škrlec, Katja; Štrukelj, Borut; Berlec, Aleš

doi:10.1016/j.tibtech.2015.03.012

Cited by 173 publications

(143 citation statements)

References 99 publications

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“…The high-affinity binders are then selected by phage display or, recently, by ribosome display (24). Examples of such scaffold-protein affinity reagents (SPARs) (25) include DARPins (designed ankyrin repeat proteins). (26), monobodies, designed on the scaffold of human fibronectin III domain 3 (27), and also Affibody molecules (28) and anticalins (29).…”

Section: Nanobodies Versus Specific Binding Proteins Designed On Non-mentioning

confidence: 99%

Nanobodies as Probes for Protein Dynamics in Vitro and in Cells

Dmitriev

Lutsenko

Muyldermans

2016

Journal of Biological Chemistry

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Nanobodies are the recombinant antigen-recognizing domains of the minimalistic heavy chain-only antibodies produced by camels and llamas. Nanobodies can be easily generated, effectively optimized, and variously derivatized with standard molecular biology protocols. These properties have triggered the recent explosion in the nanobody use in basic and clinical research. This review focuses on the emerging use of nanobodies for understanding and monitoring protein dynamics on the scales ranging from isolated protein domains to live cells, from nanoseconds to hours. The small size and high solubility make nanobodies uniquely suited for studying protein dynamics by NMR. The ability to produce conformation-sensitive nanobodies in cells enables studies that link structural dynamics of a target protein to its cellular behavior. The link between in vitro and in-cell dynamics, afforded by nanobodies, brings the analysis of such important events as receptor signaling, membrane protein trafficking, and protein interactions to the next level of resolution.

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Section: Nanobodies Versus Specific Binding Proteins Designed On Non-mentioning

confidence: 99%

Nanobodies as Probes for Protein Dynamics in Vitro and in Cells

Dmitriev

Lutsenko

Muyldermans

2016

Journal of Biological Chemistry

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“…6,7 The last years have witnessed a large number of studies attempting at developing label-free biosensors alternative to currently used platforms based on optical response. [8][9][10][11][12][13][14][15] As for IL-6, the latest reported biosensors that allow for a real-time monitoring of the cytokine levels without requiring secondary [33][34][35][36][37] .…”

Section: Introductionmentioning

confidence: 99%

Label-free detection of interleukin-6 using electrolyte gated organic field effect transistors

Diacci¹,

Berto²,

Lauro³

et al. 2017

Biointerphases

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“…However, artificial proteins for binding are usually smaller (i.e., <100 amino-acid residues) than antibodies. They have high stability with deficiency of disulfide bonds in contrast to antibodies [16,17]. Artificial proteins are thus a promising candidate to create biosensors [18,19].…”

Section: Antibody/antigen Interactionsmentioning

confidence: 99%

Recent Advances and Applications of Biosensors in Novel Technology

Rajpoot¹

2017

Biosens J

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In recent time, several novel biosensors such as enzyme based, immunosensors, tissue-based, DNA biosensors, piezoelectric, and thermal biosensors have been explored with high sensitivity by many research groups. These biosensors exhibit many essential benefits, including operational simplicity, remarkable sensitivity, low-cost instrumentation, the potential of automation, inherent miniaturization. They have offered elegant paths for the detection of even trace amounts of analytical agents of biological significance existing from macromolecules (e.g., disease biomarkers and antigens) and small molecules (e.g., natural toxins and haptens) to cells, viruses, and bacteria. Many electrochemical techniques viz., amperometry and voltammetry, photoelectrochemistry, electrochemiluminescence, impedance, piezoelectricity, potentiometry, alternating current electrohydrodynamics, and field-effect transistor have been utilized in the development of immunoassays to attain high sensitivity for electrochemical signal transduction. Recent developments in biological methods and instrumentation after using fluorescence tag to various nanocarriers such as nanoparticles, nanowires, nanotubes, etc. have improved the sensitivity of biosensors. Utilization of nucleotides/aptamers, affibodies, molecule imprinted polymers, and peptide arrays offer great tools to prepare advanced biosensors. Merging of nanotechnology with biosensor systems enhanced the diagnostic capability. This review gives an outline of the advances in biosensors technology relating biomedical sciences.

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Non-immunoglobulin scaffolds: a focus on their targets

Cited by 173 publications

References 99 publications

Nanobodies as Probes for Protein Dynamics in Vitro and in Cells

Nanobodies as Probes for Protein Dynamics in Vitro and in Cells

Label-free detection of interleukin-6 using electrolyte gated organic field effect transistors

Recent Advances and Applications of Biosensors in Novel Technology

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