Non-Human Primate Models of Tuberculosis

Peña, Juliet C.; Ho, Wen‐Zhe

doi:10.1128/microbiolspec.tbtb2-0007-2016

Cited by 36 publications

(34 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although no model perfectly recapitulates the human infection, the humanized mouse can help in identifying critical components in the human immune response that can guide future vaccine development, particularly those that are not represented in animals. The non‐human primate is becoming more widely used as the model of choice for testing vaccines; however, the limitations associated with purchase and care make this model suitable for end‐stage vaccine development, rather than an initial screen for new vaccines. Development of the humanized mouse for M. tuberculosis infection is required and warranted as an additional preclinical tool in studying various aspects of tuberculosis disease .…”

Section: Discussionmentioning

confidence: 99%

Humanized NOG mice as a model for tuberculosis vaccine‐induced immunity: a comparative analysis with the mouse and guinea pig models of tuberculosis

Grover

Troy

Rowe³

et al. 2017

Immunology

View full text Add to dashboard Cite

The humanized mouse model has been developed as a model to identify and characterize human immune responses to human pathogens and has been used to better identify vaccine candidates. In the current studies, the humanized mouse was used to determine the ability of a vaccine to affect the immune response to infection with Mycobacterium tuberculosis. Both human CD4 and CD8 T cells responded to infection in humanized mice as a result of infection. In humanized mice vaccinated with either BCG or with CpG-C, a liposome-based formulation containing the M. tuberculosis antigen ESAT-6, both CD4 and CD8 T cells secreted cytokines that are known to be required for induction of protective immunity. In comparison to the C57BL/6 mouse model and Hartley guinea pig model of tuberculosis, data obtained from humanized mice complemented the data observed in the former models and provided further evidence that a vaccine can induce a human T-cell response. Humanized mice provide a crucial pre-clinical platform for evaluating human T-cell immune responses in vaccine development against M. tuberculosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Humanized NOG mice as a model for tuberculosis vaccine‐induced immunity: a comparative analysis with the mouse and guinea pig models of tuberculosis

Grover

Troy

Rowe³

et al. 2017

Immunology

View full text Add to dashboard Cite

show abstract

“…The nonhuman primate (NHP) is another model for studies of vaccines against Mtb infection. This model is limited by high cost, ethical concerns, and specific technical requirements [11,12]. In general, NHPs are hypersusceptible to Mtb infection and demonstrate accelerated progression to disease and early death, precluding the study of the efficacy of vaccines against Mtb infection/disease caused by highly virulent clinical strains [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…This model is limited by high cost, ethical concerns, and specific technical requirements [11,12]. In general, NHPs are hypersusceptible to Mtb infection and demonstrate accelerated progression to disease and early death, precluding the study of the efficacy of vaccines against Mtb infection/disease caused by highly virulent clinical strains [11,12]. Although differential host susceptibility to Mtb infection has been reported among various species of NHP, most of the NHP studies have used either Mtb CDC1551 or Mtb Erdman as infecting strains, rather than a hypervirulent strain such as HN878 [12].…”

Section: Introductionmentioning

confidence: 99%

Inoculum size and traits of the infecting clinical strain define the protection level against Mycobacterium tuberculosis infection in a rabbit model

et al. 2020

View full text Add to dashboard Cite

Host protective immunity against pathogenic Mycobacterium tuberculosis (Mtb) infection is variable and poorly understood. Both prior Mtb infection and BCG vaccination have been reported to confer some protection against subsequent infection and/or disease. However, the immune correlates of host protection with or without BCG vaccination remain poorly understood. Similarly, the host response to concomitant infection with mixed Mtb strains is unclear. In this study, we used the rabbit model to examine the host response to various infectious doses of virulent Mtb HN878 with and without prior BCG vaccination, as well as simultaneous infection with a mixture of two Mtb clinical isolates HN878 and CDC1551. We demonstrate that both the ability of host immunity to control pulmonary Mtb infection and the protective efficacy of BCG vaccination against the progression of Mtb infection to disease is dependent on the infectious inoculum. The host response to infection with mixed Mtb strains mirrors the differential responses seen during infection with each of the strains alone. The protective response mounted against a hyperimmunogenic Mtb strain has a limited impact on the control of disease caused by a hypervirulent strain. This preclinical study will aid in predicting the success of any vaccination strategy and in optimizing TB vaccines.

show abstract

“…NHPs have been successfully used for research on specific topics such as tuberculosis, HIV central nervous system disease, hepatitis C, autism, and Parkinson's disease, as well as for broader biological subjects such as vaccine development, organ/tissue transplantation, surgical technique development, cancer research, autoimmunology research, and reproductive fertility (Burm, Collignon, Mesalam, & Meuleman, ; Lankau, Turner, Mullan, & Galland, ; Morissette & Di Paolo, ; Peña, & Ho, ; Williams, Lackner, & Mallard, ; Zhao, Jiang, & Zhang, ). Although research on the gut microbiota continues to demonstrate its role in human health and its relation to different disease states, there is limited knowledge regarding the gut microbiota along the intestinal tract of NHPs.…”

Section: Introductionmentioning

confidence: 99%

Metagenomic assessment of the Cebus apella gut microbiota

Firrman

Liu

Tanes

et al. 2019

American J Primatol

View full text Add to dashboard Cite

Cebus Apella (C. apella) is a species of Nonhuman Primate (NHP) used for biomedical research because it is phylogenetically similar and shares anatomical commonalities with humans. Here, the gut microbiota of three C. apella were examined in the different regions of the intestinal tract. Using metagenomics, the gut microbiota associated with the luminal content and mucus layer for each intestinal region was identified, and functionality was investigated by quantifying the levels of short chain fatty acids (SCFAs) produced. The results of this study show a high degree of similarity in the intestinal communities among C. apella subjects, with multiple shared characteristics. First, the communities in the lumen were more phylogenetically diverse and rich compared to the mucus layer communities throughout the entire intestinal tract. The small intestine communities in the lumen displayed a higher Shannon diversity index compared to the colon communities. Second, all the communities were dominated by aero-tolerant taxa such as Streptococcus, Enterococcus, Abiotrophia, and Lactobacillus, although there was preferential colonization of specific taxa observed. Finally, the primary SCFA produced throughout the intestinal tract was acetic acid, with some propionic acid and butyric acid detected in the colon regions. The small intestine microbiota produced significantly less SCFAs compared to the communities in the colon. Collectively, these data provide an in-depth report on the composition, distribution, and SCFA production of the gut microbiota along the intestinal tract of the C. apella NHP animal model. K E Y W O R D SCebus apella, gut microbiota, in vivo studies, intestinal tract, primate research, tufted capuchin

show abstract

Non-Human Primate Models of Tuberculosis

Cited by 36 publications

References 65 publications

Humanized NOG mice as a model for tuberculosis vaccine‐induced immunity: a comparative analysis with the mouse and guinea pig models of tuberculosis

Humanized NOG mice as a model for tuberculosis vaccine‐induced immunity: a comparative analysis with the mouse and guinea pig models of tuberculosis

Inoculum size and traits of the infecting clinical strain define the protection level against Mycobacterium tuberculosis infection in a rabbit model

Metagenomic assessment of the Cebus apella gut microbiota

Contact Info

Product

Resources

About