2020
DOI: 10.1111/pbi.13367
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Non‐GMO potato lines, synthesizing increased amylose and resistant starch, are mainly deficient in isoamylase debranching enzyme

Abstract: Summary Solanum tuberosum potato lines with high amylose content were generated by crossing with the wild potato species Solanum sandemanii followed by repeated backcrossing to Solanum tuberosum lines. The trait, termed increased amylose (IAm), was recessive and present after three generations of backcrossing into S. tuberosum lines (6.25% S. sandemanii genes). The tubers of these lines were small, elongated and irregular with small and misshaped starch granules and high sugar content. Additional backcrossing … Show more

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Cited by 18 publications
(7 citation statements)
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“…SBEI seems to prefer amylose as a substrate and, as a consequence, suppression of SBEI increased the relative amylose content. ISAII suppression can cause high amylose content by decreasing the amylopectin content, e.g., as demonstrated, a potato mutant deficient in this enzyme …”
Section: Resultsmentioning
confidence: 69%
“…SBEI seems to prefer amylose as a substrate and, as a consequence, suppression of SBEI increased the relative amylose content. ISAII suppression can cause high amylose content by decreasing the amylopectin content, e.g., as demonstrated, a potato mutant deficient in this enzyme …”
Section: Resultsmentioning
confidence: 69%
“…Previous studies have proposed that, for high amylose maize starches, amylose is more concentrated at the periphery than the core, while the opposite is observed for normal and waxy starches [54]. However, such AM distributions can be complex depending on genotypes and mutations in the genes coding for target starch metabolizing enzymes [55][56][57]. Such knowledge, such as the degree of branching and chain length distribution on the surface, is crucial for starch hydrolysis, as the flexible glucan chains on the granule surface are considered a primary factor influencing the susceptibility of starch to amylolysis [58].…”
Section: Starch Granular Topography and Morphologymentioning
confidence: 99%
“…Enzymatic hydrolysis of starch granules is a complex and heterogeneous catalytic process [64] that depends on the interplay among the widely different granule surfaces and matrix structures described above, the substrate recognition and catalysis by the hydrolase. Importantly, at the granular starch surface, a fraction of glucan chains may be accessible, which potentially provides efficient binding sites and substrates for starch-active enzymes [56,57]. The rate of hydrolysis can be controlled by three main factors: (i) diffusion of the enzyme toward the granule surface; (ii) adsorption onto structures at the granule surface; and (iii) catalytic glycoside bond hydrolysis (Figure 3).…”
Section: Hydrolase Mechanisms On Granular Substratesmentioning
confidence: 99%
“…Two factors lead to the increased amylose content in these mutants: First, as BE is required for amylopectin biosynthesis, suppressing its activity reduces the amount of amylopectin and increases the relative proportion of amylose. Similarly, many mutations in the amylopectin biosynthesis pathway have been observed to increase amylose content in Arabidopsis leaves and various crops: including other starch synthases (Wang et al ., 1993; Delvallé et al ., 2005; Fujita et al ., 2007; Zhang et al ., 2008; Szydlowski et al ., 2009), and isoamylase (Dauvillée et al ., 2001; Tziotis et al ., 2004; Blennow et al ., 2020). Second, the suppression of BE reduces the frequency of branching on amylopectin, resulting in the formation of long amylose‐like chains on amylopectin.…”
Section: Factors That Influence Amylose Contentmentioning
confidence: 99%