Non-Fibrous Desoxypentose Nucleohistone From Liver

Kupke, Donald W.; Eldredge, Noreen T.; Luck, J. Murray

doi:10.1016/s0021-9258(18)65454-7

Cited by 5 publications

(1 citation statement)

References 16 publications

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“…It now seems clear that the easily extractable nucleohistone, such as that described by Luck eta]. (12,13) is in a sense an artifact produced by the isolation procedure, since some factor (generally the mitochondrlal enzyme) must liberate the DNA from the protein to which it is normally attached in order for it to become easily extractable together with the histone in strong salt solutions. Work is in progress in our laboratory which it is hoped will show definitely to which component of the nuclear protein the DNA is bound.…”

Section: Discussionmentioning

confidence: 99%

Factors Influencing the Ability of Isolated Cell Nuclei to Form Gels in Dilute Alkali

Dounce

Monty

1955

The Journal of Cell Biology

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1. Known methods for isolating cell nuclei are divided into two classes, depending on whether or not the nuclei are capable of forming gels in dilute alkali or strong saline solutions. Methods which produce nuclei that can form gels apparently prevent the action of an intramitochondrial enzyme capable of destroying the gel-forming capacity of the nuclei. Methods in the other class are believed to permit this enzyme to act on the nuclei during the isolation procedure, causing detachment of DNA from some nuclear constituent (probably protein). 2. It is shown that heating in alkaline solution and x-irradiation can destroy nuclear gels. Heating in acid or neutral solutions can destroy the capacity of isolated nuclei to form gels. 3. Chemical and biological evidence is summarized in favor of the hypothesis that DNA is normally bound firmly to some nuclear component by non-ionic linkages.

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Section: Discussionmentioning

confidence: 99%