Non-fermenting gram negative bacilli isolated from blood and their in vitro sensitivity pattern in a tertiary care hospital

Abstract

Background: Bacteremia caused by non-fermenting gram negative bacilli (NFGNB) is a serious infection which is associated with high morbidity and mortality. Identification of NFGNB and monitoring their susceptibility pattern are important for proper management of these infections. Aim: Scope of the present study is to characterize the NFGNB along with their antimicrobial sensitivity pattern among the patients coming to our hospital, a tertiary care center. Materials & Methods: A retrospective study was conducted to determine the NFGNB isolated from blood during the one-year study period. A total of 8223 Blood cultures were received in the laboratory which was processed by semi-automated technique in Bac-T Alert. Antibiotic susceptibility was performed by VITEK and Kirby Bauer methods and interpreted by CLSI guidelines. 102 isolates of NFGNB were obtained from the Blood culture. Results: Most commonly isolated organism is Acinetobacter species (47%) followed by Pseudomonas spp (27%) and Burkholderia spp(15.6%). Maximum isolates of Acinetobacter spp were sensitive to colistin (91.9%), cefoperazone-sulbactum (88.4%), Tigecycline (62.5%). Pseudomonas spp was sensitive to colistin (100%), ciprofloxacin (59%). Conclusion: Identification of NFGNB and monitoring their susceptibility patterns will help in proper management of infections caused by them.

A study on clinico-demographic and microbiological profile of surgical site infection (SSI) in a tertiary care hospital, Kolkata: An alarming trend

“…In contrast to the three preceding studies, 14,16,18 the present study reveals a notably higher proportion of MRSA, multi-drug resistant Klebsiella pneumoniae and Acinetobacter baumannii complex isolations from SSI samples indicating a matter of concern.…”

“…Proportion of isolated organisms from previous three studies from same institute 14,16,18 and current study is compared in Figure 5.…”

“…Sikdar S et al demonstrated that Klebsiella pneumoniae and Escherichia coli were predominant pathogens of SSI 18 and Deb S et al showed nonfermenters like Pseudomonas aeruginosa and Acinetobacter baumannii complex as emerging pathogens of SSI. 16 Isolation of enteric pathogens, e.g., Escherichia coli which are part of patient's endogenous flora, and nonfermenter Pseudomonas aeruginosa which thrives in warmer healthcare environment, indicate poor hospital hygiene practices. 31 The susceptibility pattern of Gram-positive cocci showed resistance to penicillin (95.18%) but higher susceptibility has been recorded against daptomycin (99.22%), linezolid (98.64%), teicoplanin (96.96%) and vancomycin (96.67%).…”

“…13,14 Recent reports have shown that multidrug-resistant (MDR) Gram-negative bacilli and non-fermenters like Pseudomonas aeruginosa and Acinetobacter baumannii complex are increasingly isolated from SSI. 15,16 Hence, aim of this study was to determine magnitude of SSI in various surgical specialities, predominant pathogens causing SSI and their antibiotic susceptibility patterns.…”