Non-Faradaic electrochemical detection of protein interactions by integrated neuromorphic CMOS sensors

Jacquot, Blake C.; Muñoz, Nini; Branch, Darren W.; Kan, Edwin C.

doi:10.1016/j.bios.2008.01.006

Cited by 20 publications

(8 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[78] Streptavidin binding to BSA could be detected using chemoreceptive neuron MOS devices based on the technology of field effect transistor sensors. [79] Microchips with three-dimensional gold micro electrodes were used to detect the dynamic process of cell membrane permeability during electroporation and membrane recovery. This technique avoids the manipulation of the cells using a pipette (patch-clamp technique) circumventing concomitantly the limitation of optical methods.…”

Section: Advanced Eis Techniquesmentioning

confidence: 99%

Biomaterial Interface Investigated by Electrochemical Impedance Spectroscopy

2008

View full text Add to dashboard Cite

Electrochemical impedance spectroscopyElectrochemical impedance spectroscopy is an easy applicable sensitive technique. It is used to get information about the adsorption behaviour and the interactions of biomolecules with the electrode surface in a short measurement duration. Electrochemical processes can be modelled by an equivalent circuit and various parameters reflecting certain properties of the interface can be determined. The electrochemical properties of the interface are influenced by proteins and cells adsorbing at the surface. Different modified surfaces can be analyzed before, during, and after cell contact, respectively. Applying bias potentials also changes the behaviour of attaching cells and proteins. Changes in the medium composition have an influence on the electrochemically detectable surface properties.Biosensors are developed consecutively to detect biomolecules. Enzymes, lipids and membranes can be analyzed and the adsorption behaviour of DNA and antigens at modified electrode surfaces can be determined. Different technologies using microchips were constructed to allow a fast screening of electrochemical properties and discrimination of biological molecules or cells. ApplicationElectrochemical impedance spectroscopy serves not only for determination of the capacity of new battery materials or corrosion behaviour and long term stability of diverse alloys and oxide layers, it can also be used to gain information about biomolecules. The adsorption behaviour of cells [1][2][3] and proteins, [4] the amount of adsorbed proteins, [4][5][6][7] surface charge densities [4][5][6] and adsorption coefficients [5] can be determined. Interactions of implant surfaces with biological devices, changes in the polarizability of functional groups, the transport of ions or dipoles through polymer layers and mineralization processes, for instance of bone tissue can be assessed. The change of the Gibbs free energy and the entropy due to protein binding to the surface can be calculated [4,6,8] and the affinity of biomolecules to the surface can be estimated. [4] Electrochemical techniques are introduced as biosensors. Antigens, ssDNA or enzymes are immobilized at the surface and according antibodies, DNA and distinct proteins can be ADVANCED ENGINEERING MATERIALS 2008, 10, No. 10

show abstract

Section: Advanced Eis Techniquesmentioning

confidence: 99%

Biomaterial Interface Investigated by Electrochemical Impedance Spectroscopy

2008

View full text Add to dashboard Cite

show abstract

“…One of the unique features of the neuron transistors is the ultralow power dissipation during calculation due to the gate-level sum operation in a voltage mode. From then on, Si-based neuron transistors have attracted much attention for chemical and biological detection due to the easy adjustment of threshold voltage 16 17 18 19 20 . When an asymmetric gate capacitor structure is adopted, magnification of V th shift can be observed in the neuron transistor when the sensing gate experiences a load from electrolyte.…”

mentioning

confidence: 99%

Flexible Sensory Platform Based on Oxide-based Neuromorphic Transistors

Ning

Zhu

Feng

et al. 2015

Sci Rep

View full text Add to dashboard Cite

Inspired by the dendritic integration and spiking operation of a biological neuron, flexible oxide-based neuromorphic transistors with multiple input gates are fabricated on flexible plastic substrates for pH sensor applications. When such device is operated in a quasi-static dual-gate synergic sensing mode, it shows a high pH sensitivity of ~105 mV/pH. Our results also demonstrate that single-spike dynamic mode can remarkably improve pH sensitivity and reduce response/recover time and power consumption. Moreover, we find that an appropriate negative bias applied on the sensing gate electrode can further enhance the pH sensitivity and reduce the power consumption. Our flexible neuromorphic transistors provide a new-concept sensory platform for biochemical detection with high sensitivity, rapid response and ultralow power consumption.

show abstract

“…This FETtype sensor provided a convenient and sensitive method to detect binding of Hsp90 inhibitors to Hsp90 in real-time (Jacquot et al, 2008). Hsp90-CPNT FET sensor will offer not only the feasibility of anti-cancer agent detection, but also novel nanoscale sensor to investigate small molecule-protein interactions, which is critical for both basic and applied biological researches.…”

Section: Introductionmentioning

confidence: 99%