Non-esterified fatty acids in early luteal bovine oviduct fluid mirror plasma concentrations: An ex vivo approach

Jordaens, L.; Hoeck, Veerle Van; Bie, Jessie De; Berth, Mario; Marei, Waleed F.A.; Desmet, K. L. J.; Bols, P.E.J.; Leroy, Jo

doi:10.1016/j.repbio.2017.05.009

Cited by 18 publications

(20 citation statements)

References 13 publications

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“…Intensive energy demand is often manifested through elevated non-esterified fatty acids (NEFAs) in the circulating blood. In healthy cattle, levels of NEFAs in the tubal fluid mirror the concentrations measured in plasma [30], meaning that, in the case of lipolytic metabolism during the periconceptional period, the early embryo is likely exposed to elevated NEFAs. Elevated levels of NEFAs were shown to affect embryo DNA methylation and gene expression [103].…”

Section: Metabolism and Energy Balancementioning

confidence: 85%

“…The embryo lipid reserve may be of particular importance in species with long preimplantation period such as pigs, cattle, dogs, and horses. Oviductal secretions contain a variety of lipids, including cholesterol, triglycerids, and fatty acids [30]. The OF contains also L-carnitine, which is required for the beta-oxydation of these lipids, while albumin as well as high-(HDLs) and low-density lipoproteins (LDLs) play the role of lipid carriers in OF [25,31].…”

Section: Lipidsmentioning

confidence: 99%

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Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Saint‐Dizier

Schoen

Chen

et al. 2019

IJMS

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The oviductal fluid is the first environment experienced by mammalian embryos at the very beginning of life. However, it has long been believed that the oviductal environment was not essential for proper embryonic development. Successful establishment of in vitro embryo production techniques (which completely bypass the oviduct) have reinforced this idea. Yet, it became evident that in vitro produced embryos differ markedly from their in vivo counterparts, and these differences are associated with lower pregnancy outcomes and more health issues after birth. Nowadays, researchers consider the oviduct as the most suitable microenvironment for early embryonic development and a substantial effort is made to understand its dynamic, species-specific functions. In this review, we touch on the origin and molecular components of the oviductal fluid in mammals, where recent progress has been made thanks to the wider use of mass spectrometry techniques. Some of the factors and processes known to regulate oviductal secretions, including the embryo itself, as well as ovulation, insemination, endogenous and exogenous hormones, and metabolic and heat stress, are summarized. Special emphasis is laid on farm animals because, owing to the availability of sample material and the economic importance of fertility in livestock husbandry, a large part of the work on this topic has been carried out in domestic animals used for dairy and/or meat production.

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Section: Metabolism and Energy Balancementioning

confidence: 85%

Section: Lipidsmentioning

confidence: 99%

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Saint‐Dizier

Schoen

Chen

et al. 2019

IJMS

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“…During the early luteal phase, OF is composed of cholesterol, triglycerides, non-esterified fatty acids (NEFA), proteins, glucose, and lactate. OF and plasma have similar concentrations of NEFA in healthy cows that probably alter the lipid composition of oviduct cells [88]. Alterations in the oviduct microenvironment can affect embryo development; thus, it is comprehensible that alterations in feed composition also affect pregnancy establishment.…”

Section: Tubal Fluidsmentioning

confidence: 99%

Lipid Metabolism in Bovine Oocytes and Early Embryos under In Vivo, In Vitro, and Stress Conditions

Sterza

Poehland

2021

IJMS

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Lipids are a potential reservoir of energy for initial embryonic development before activation of the embryonic genome and are involved in plasma membrane biosynthesis. Excessive lipid droplet formation is detrimental to cryotolerance and is related to alterations in mitochondrial function, which likely affects lipid metabolism. Increased lipid accumulation in in vitro produced embryos is a consequence of the stress during in vitro embryonic development process. There are several open questions concerning embryo lipid metabolism and developmental potential. Oocyte maturation and embryo development in vivo and in vitro may vary if the donors are subjected to any type of stress before follicle puncture because crucial changes in oocyte/embryonic metabolism occur in response to stress. However, little is known about lipid metabolism under additional stress (such as heat stress). Therefore, in this review, we aimed to update the information regarding the energy metabolism of oocytes and early bovine embryos exhibiting developmental competence, focusing on lipid metabolic pathways observed under in vivo, in vitro, and stress conditions.

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“…In order to evaluate the effect of elevated NEFA exposure during sperm capacitation (4 h) and fertilization (22 h), fertilization medium was supplemented with the following NEFA treatments: i) STANDARD: FA-free and solvent-free medium, ii) SOLVENT: FA-free medium with 0.45 % absolute ethanol, Since no data are available regarding the concentrations of FFAs in oviductal fluid in cows under metabolic stress conditions, the concentrations of FFAs used here are based on serum concentrations in cows during NEB (Leroy et al 2005) which may correlate with the oviductal fluid concentrations (Jordaens et al 2017a). A basal NEFA treatment group was implemented as a control in this study in order to improve the physiological relevance of our in vitro model as these NEFAs are present at basal concentrations in the environment in which oocytes mature and embryos develop in vivo (Leroy et al 2005).…”

Section: Experimental Designmentioning

confidence: 99%

“…Likewise, early bovine embryos are highly sensitive to changes in their micro-environment, the oviduct (Rizos et al 2003;Leese et al 2008). As the maternal metabolic state is reflected in blood and follicular fluid, it may furthermore influence oviductal fluid composition; a recent study showed that NEFAs are present in the oviductal fluid and mirror the concentrations seen in plasma of healthy cows (Jordaens et al 2017a). Although the oviduct has been suggested to modulate its micro-environment by alleviating potential lipotoxic effects (Jordaens et al 2017b), it remains debatable whether this gatekeeper function is sufficient to actually protect the embryo from an adverse climate.…”

Section: Introductionmentioning

confidence: 99%

The effect of elevated non-esterified fatty acid concentrations on bovine spermatozoa and on oocyte in vitro fertilisation

Desmet¹,

Marei²,

Pintelon³

et al. 2018

Reprod. Fertil. Dev.

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Elevated non-esterified fatty acid (NEFA) concentrations, present in follicular and oviductal fluid, have been postulated as a causative link between metabolic disorders and subfertility. High NEFA conditions can directly disrupt oocyte maturation and developmental capacity after fertilisation. However, their influence on sperm function and the fertilisation process is not known. This study investigated the fertilisation process under high NEFA conditions. To differentiate between effects on both spermatozoa and oocytes or on spermatozoa only, different experiments were conducted. In the first experiment both gametes were simultaneously incubated during IVF under different conditions: (1) NEFA-free, solvent-free control conditions, (2) solvent control, (3) physiological concentrations of oleic (OA), palmitic (PA) and stearic (SA) acids or (4) pathophysiological concentrations of OA, PA and SA. In the second experiment spermatozoa were incubated (4h) under the same treatment conditions prior to routine IVF. Gamete co-incubation resulted in reduced fertilisation and cleavage rates and increased prevalence of polyspermy. In the second experiment embryo developmental capacity and quality were not affected, although sperm motility and plasma membrane integrity were decreased. In conclusion, lipolytic conditions affected the fertilisation process mainly through an effect on the oocyte. Spermatozoa were still able to fertilise even though these conditions reduced sperm function.

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Non-esterified fatty acids in early luteal bovine oviduct fluid mirror plasma concentrations: An ex vivo approach

Cited by 18 publications

References 13 publications

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Lipid Metabolism in Bovine Oocytes and Early Embryos under In Vivo, In Vitro, and Stress Conditions

The effect of elevated non-esterified fatty acid concentrations on bovine spermatozoa and on oocyte in vitro fertilisation

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