Non-enzymatic electrochemical detection of uric acid with electrodeposited Nafion film

Xu, Zheng; Mengqi, Zhang; Zou, Hong-qun; Liu, Junshan; Wang, Dazhi; Wang, Jing; Wang, Liding

doi:10.1016/j.jelechem.2019.04.028

Cited by 30 publications

(15 citation statements)

References 38 publications

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“…We found that urate decreased the measured current from the sensor, indicating that it may interfere with oxygen measurements. There is conflicting literature regarding Nafion permeability to urate, with several reports showing that Nafion improves selectivity for catecholamine neurotransmitter measurements in the presence of urate [47], while others have shown that it can be used to block ascorbate and allow selective measurement of urate [48][49][50]. Our data suggest that the Nafion sensor membrane is permeable to urate, likely in its protonated uric acid form as it would otherwise be electrostatically excluded, and may become deposited within the hydrated pores limiting mass transport.…”

Section: Discussionmentioning

confidence: 67%

Real-time measurement of tumour hypoxia using an implantable microfabricated oxygen sensor

Marland¹,

Gray²,

Dunare³

et al. 2020

Preprint

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Hypoxia commonly occurs within tumours and is a major cause of radiotherapy resistance. Clinical outcomes could be improved by locating and selectively increasing the dose delivered to hypoxic regions. Here we describe a miniature implantable sensor for real-time monitoring of tissue oxygenation that could enable this novel treatment approach to be implemented. The sensor uses a solid-state electrochemical cell that was microfabricated at wafer level on a silicon substrate, and includes an integrated reference electrode and electrolyte membrane. It gave a linear response to oxygen concentration, and was unaffected by sterilisation and irradiation, but showed susceptibility to biofouling. Oxygen selectivity was also evaluated against various clinically relevant electroactive compounds. We investigated its robustness and functionality under realistic clinical conditions using a sheep model of lung cancer. The sensor remained functional following CT-guided tumour implantation, and was sufficiently sensitive to track acute changes in oxygenation within tumour tissue.

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Section: Discussionmentioning

confidence: 67%

Real-time measurement of tumour hypoxia using an implantable microfabricated oxygen sensor

Marland¹,

Gray²,

Dunare³

et al. 2020

Preprint

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“…Thus, NH 2 -VMSF/ErGO/SPCE sensor exhibits higher sensitivity and lower LOD, which is mainly due to the further enrichment of nanochannels. The LOD obtained on NH 2 -VMSF/ErGO/SPCE was lower than that obtained on Nafion-modified SPCE [ 33 ], nanodiamond modified SPE [ 34 ], Uricase/graphene-incorporated chitosan/Prussian blue modified SPCE (Uricase/Chi-Gr/PB/SPCE) [ 35 ], uricase-inorganic hybrid nanoflowers-Au nanoparticles modified SPCE (UOx-NFs-AuNPs-SPCE) [ 36 ], and Co 3 O 4 -ErGO modified SPE (Co 3 O 4 -ErGO/SPE) [ 37 ]. The low LOD of the developed sensor lies in the dual amplification effects resulting from ErGO and nanochannels.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Sensor Nanoarchitectonics for Sensitive Detection of Uric Acid in Human Whole Blood Based on Screen-Printed Carbon Electrode Equipped with Vertically-Ordered Mesoporous Silica-Nanochannel Film

Yang

Liu

et al. 2022

Nanomaterials

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Screen-printed carbon electrodes (SPCEs) bear great potential in the detection of biomarker in clinical samples with low sample consumption. However, modification of electrode surfaces to improve the anti-interference ability and sensitivity is highly desirable for direct electroanalysis of whole blood samples. Here, a reliable and miniaturized electrochemical sensor is demonstrated based on SPCE equipped with vertically-ordered mesoporous silica-nanochannel film (VMSF). To achieve stable binding of VMSF and improve the electrocatalytic performance, electrochemically reduced graphene oxide (ErGO) is applied as a conductive adhesion layer, that is in situ reduced from GO nanosheets during fast growth (less than 10 s) of amino groups modified VMSF (NH2-VMSF) using electrochemically assisted self-assembly (EASA). In comparison with bare SPCE, NH2-VMSF/ErGO/SPCE exhibits decreased oxidation potential of uric acid (UA) by 147 mV owing to significant electrocatalytic ability of ErGO. The dual signal amplification based on electrocatalysis of ErGO and enrichment of nanochannels leads to enhanced peak current by 3.9 times. Thus, the developed NH2-VMSF/ErGO/SPCE sensor enables sensitive detection of UA in the range from 0.5 μM to 180 μM with a low limit of detection (LOD, 129 nM, S/N = 3). Owing to good anti-fouling ability and high selectivity of the sensor, direct and rapid detection of UA in human whole blood is realized with very low sample consumption (50 μL).

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“…Since the carbon material used for the printing has a very porous structure even after printing (as shown in Section 3.4), the electrode needed to be tested against an organic electroactive molecule to evaluate the presence of any fouling of the surface due to the interaction between the carbon-based working electrode surface and the electrochemical products. Uric acid was chosen as the target electroactive organic molecule, as it is known from the literature [42] that it spontaneously oxidizes in aqueous media when a potential of 0.2 V or higher is applied, forming allantoin. This substance, thanks to its numerous electron attractor and electron donor groups, is a good candidate to see if the developed WE suffers from fouling due to the formation of stable interactions with electrochemical products which result in reduced electrochemical performances of the electrode.…”

Section: Carbonized Lignin Spe Test As a Sensor Platformmentioning

confidence: 99%

Innovative Eco-Friendly Conductive Ink Based on Carbonized Lignin for the Production of Flexible and Stretchable Bio-Sensors

et al. 2021

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In this study, we report a novel way to produce carbon-based conductive inks for electronic and sensor technology applications. Carbonized lignin, obtained from the waste products of the Eucalyptus globulus tree paper industry, was used to produce a stable conductive ink. To this end, liquid-phase compositions were tested with different amounts of carbonized lignin powder to obtain an ink with optimal conductivity and rheological properties for different possible uses. The combination that showed the best properties, both regarding electrochemical properties and green compatibility of the materials employed, was cyclohexanone/cellulose acetate/carbonized lignin 5% (w/w), which was used to produce screen-printed electrodes. The electrodes were characterized from a structural and electrochemical point of view, resulting in an electrochemically active area of 0.1813 cm2, compared to the electrochemically active area of 0.1420 cm2 obtained by employing geometrically similar petroleum-based screen-printed electrodes and, finally, their performance was demonstrated for the quantification of uric acid, with a limit of detection of 0.3 μM, and their biocompatibility was assessed by testing it with the laccase enzyme and achieving a limit of detection of 2.01 μM for catechol as the substrate. The results suggest that the developed ink could be of great use in both sensor and electronic industries, reducing the overall ecological impact of traditionally used petroleum-based inks.

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Non-enzymatic electrochemical detection of uric acid with electrodeposited Nafion film

Cited by 30 publications

References 38 publications

Real-time measurement of tumour hypoxia using an implantable microfabricated oxygen sensor

Real-time measurement of tumour hypoxia using an implantable microfabricated oxygen sensor

Electrochemical Sensor Nanoarchitectonics for Sensitive Detection of Uric Acid in Human Whole Blood Based on Screen-Printed Carbon Electrode Equipped with Vertically-Ordered Mesoporous Silica-Nanochannel Film

Innovative Eco-Friendly Conductive Ink Based on Carbonized Lignin for the Production of Flexible and Stretchable Bio-Sensors

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