Non-endoscopic biopsy techniques: a review

Otuya, David O.; Verma, Yogesh Kumar; Farrokhi, Hamid; Rosenberg, Mireille; Damman, Christopher J.; Tearney, Guillermo J.

doi:10.1080/17474124.2018.1412828

Cited by 16 publications

(12 citation statements)

References 42 publications

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“…Altogether, those results show high accuracy of the transcriptomic data to differentiate celiac from non-celiac control biopsies. Such transcriptomics-based methodology can be applied on suboptimally oriented biopsies to increase accuracy of celiac diagnosis, and if future non-endoscopic sampling devices to obtain duodenal mucosal cells 25 will be introduced clinically.…”

Section: Resultsmentioning

confidence: 99%

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Defining the Celiac Disease Transcriptome using Clinical Pathology Specimens Reveals Biologic Pathways and Supports Diagnosis

Loberman-Nachum

Sosnovski

Segni

et al. 2019

Sci Rep

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Celiac disease is provoked by gluten exposure, but the complete pathogenic process in the duodenum and the loss of tolerance to gluten is not well understood. We aimed to define the core celiac transcriptomic signature and pathologic pathways in pre-treatment formalin-fixed paraffin-embedded (FFPE) duodenum biopsies used for clinical diagnosis. We use mRNAseq to define pre-treatment diagnostic duodenum gene expression in 54 pediatric celiac patients and non-celiac controls, and we validate our key findings in two independent cohorts of 67 adults and pediatric participants that used fresh frozen biopsies. We further define similar and divergent genes and pathways in 177 small bowel Crohn disease patients and controls. We observe a marked suppression of mature epithelial metabolic functions in celiac patients, overlapping substantially with the Crohn disease signature. A marked adaptive immune response was noted for the up-regulated signature including interferon response, alpha-beta, and gamma-delta T-cells that overlapped to some extent with the Crohn disease signature. However, we also identified a celiac disease specific signature linked to increased cell proliferation, nuclear division, and cell cycle activity that was localized primarily to the epithelia as noted by CCNB1 and Ki67 staining. Lastly, we demonstrate the utility of the transcriptomic date to correctly classify disease or healthy states in the discovery and validation cohorts. Our data supplement recently published datasets providing insights into celiac pathogenesis using clinical pathology FFPE samples, and can stimulate new approaches to address this highly prevalent condition.

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Section: Resultsmentioning

confidence: 99%

“…Finally, we show high accuracy of the transcriptomic data to differentiate celiac from non-celiac control biopsies. If future attempts for non-endoscopic sampling device 25 to obtain duodenal mucosal cells will be successful, such transcriptomic approach can aid in accurate diagnosis of celiac subjects, in conjunction with celiac serology.…”

Section: Discussionmentioning

confidence: 99%

Defining the Celiac Disease Transcriptome using Clinical Pathology Specimens Reveals Biologic Pathways and Supports Diagnosis

Loberman-Nachum

Sosnovski

Segni

et al. 2019

Sci Rep

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“…Several other electromagnetic-based sensing modules can also potentially be integrated into WCE [21]- [24]. For the tissue level diagnostics, several biopsy mechanisms for WCE have been also introduced [25]- [28] and therapeutic ones have drawn significant attention from researchers developing targeted drug delivery [29]- [32].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, several biopsy solutions for capsule endoscope have been reported to address the remaining challenges, including the size, energy consumption, and performance [28]. First, in 2005 a rotational biopsy module by Kong et al was reported.…”

Section: Introductionmentioning

confidence: 99%

Untethered Robotic Motion and Rotating Blade Mechanism for Actively Locomotive Biopsy Capsule Endoscope

Hoang

Kim

et al. 2019

IEEE Access

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Capsule endoscopy (CE) is a convenient and promising alternative endoscopic method for the gastrointestinal diagnostics on clinical sites; however, multifunctional utilization with active targeting locomotion is still challenging due to internal power limitations and micro-size manipulation mechanisms. The biopsy is one of the most demanding functions of intestinal CE, and successful developments could significantly improve the intestinal diagnostics for precise decisions and comfortable usage. In this paper, we present a novel active locomotive robotic biopsy capsule endoscope using an externally driven electromagnetic actuation (EMA) system and a battery-free wireless rotating blade mechanism. The external magnetic field from the EMA system controls both locomotion and biopsy operation, and thus, the proposed mechanism does not consume the internal power of the CE. We prototyped the proposed biopsy module and integrated it into an active locomotive capsule endoscope. Simulations were conducted to design the module and analyze the magnetic responses. We performed experiments with the biopsy capsule endoscope both in vitro and ex vivo to demonstrate its feasibility. In conclusion, the prototyped robotic biopsy capsule endoscope could successfully perform the necessary movements for the capsule in the small intestine and perform a biopsy at the target lesion with sufficient force. The amount of biopsy tissue was sufficient for a histological examination. INDEX TERMS Biopsy capsule endoscope, wireless capsule endoscope, electromagnetic actuation system, intestinal diagnosis.

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“…The approaches described above are patently different from those instances in which live study subjects have preconsented to a single-or multiple-tissue biopsy procedure in support of drug-metabolizing enzyme and transporter profiling (Lefor and Flowers, 1994;Padoin et al, 2006;Otuya et al, 2018). Alternatively, some investigators have profiled frozen (banked) tissue biopsy samples (Supplemental Fig.…”

Section: Introductionmentioning

confidence: 99%

Profiling of Drug-Metabolizing Enzymes and Transporters in Human Tissue Biopsy Samples: A Review of the Literature

Rodrigues

Rowland

2019

J Pharmacol Exp Ther

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Within the drug pharmacokinetics (PK)-absorption, distribution, metabolism, and excretion (ADME) research community, investigators regularly generate in vitro data sets using appropriately vendor-sourced and processed human tissue. Such data enable drug screening, the generation of kinetic parameters, extrapolation of in vitro to in vivo, as well as the modeling and simulation of drug PK. Although there are large numbers of manuscripts describing studies with deceased organ donor tissue, relatively few investigators have published studies utilizing living donor tissue biopsy samples. After a review of the available literature, it was possible to find publications describing the use of tissue biopsy samples to determine enzyme inhibition ex vivo, the study of genotype-phenotype associations, the evaluation of tissue expression profiling following an inducer, and assessment of correlations between tissue expression profiles and in vivo-derived trait measures (e.g., biomarker plasma levels and probe drug PK). Some reports described multiple single-tissue biopsies, whereas others described single multiple-organ biopsies. It is concluded that biopsy-derived data can support modeling exercises (as input data and when validating models) and enable the assessment of organ-specific changes in enzyme and transporter profiles resulting from drug interactions, disease (e.g., metabolic disease, fibrosis, inflammation, cancer, infection), age, pregnancy, organ impairment, and genotype. With the emergence of multiorgan axes (e.g., microbiome-gut-liverkidney) and interest in remote sensing (interorgan communication), it is envisioned that there will be increased demand for single-and multiorgan tissue biopsy data to support hypothesis testing and PK-ADME model building. SIGNIFICANCE STATEMENTBased on a review of the literature, it is apparent that profiling of human tissue biopsy samples is useful in support of pharmacokinetics (PK)-absorption, distribution, metabolism, and excretion (ADME)-related studies. With conventional tissue biopsy as precedent, it is envisioned that researchers will turn to less invasive "liquid biopsy" methods in support of ADME-related studies (e.g., profiling of plasma-derived tissue-specific nanovesicles). Generation of such multiorgan liquid biopsy data in larger numbers of subjects and at multiple study time points will provide a rich data set for modeling purposes.

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Non-endoscopic biopsy techniques: a review

Cited by 16 publications

References 42 publications

Defining the Celiac Disease Transcriptome using Clinical Pathology Specimens Reveals Biologic Pathways and Supports Diagnosis

Defining the Celiac Disease Transcriptome using Clinical Pathology Specimens Reveals Biologic Pathways and Supports Diagnosis

Untethered Robotic Motion and Rotating Blade Mechanism for Actively Locomotive Biopsy Capsule Endoscope

Profiling of Drug-Metabolizing Enzymes and Transporters in Human Tissue Biopsy Samples: A Review of the Literature

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