Non-Effect of an Antibiotic Treatment on Dietary Fiber-Induced Goblet Cell Proliferation in the Ileum of Rats

Hino, Shingo; Saita, Hiroyoshi; Morita, Tatsuya

doi:10.1271/bbb.130241

Cited by 2 publications

(3 citation statements)

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“…This hypothesis was the reason behind the antibiotics treatment we applied in the subsequent experiment to suppress the action of SCFA and microbiota in the large intestine, and which would aid in the understanding of the relationship between the anti-obesity effect of STP and the modification of intestinal fermentation. Indeed, Hino et al reported a dramatic decrease in caecal organic acid concentration in rats treated with an antibiotic cocktail ( penicillin, neomycin and cefoperazone) for 7 d. 25 The treatment reduced the food intake, which was likely due to the strong flavour and taste of the antibiotics, potentially causing the rats to eat less.…”

Section: Discussionmentioning

confidence: 99%

“…† Half of the rats in each group had access to each experimental diet ad libitum for 15 d in the presence (n = 6) or absence (n = 6) of an antibiotics cocktail (50 kU penicillin G potassium per L, 2 g neomycin sulphate per L (both from Wako Pure Chemical Industries, Ltd, Osaka Japan) and 0.5 g cefoperazone sodium salt per L; Sigma-Aldrich, St. Louis, MO, USA) added to the drinking water. 25 Faeces were collected, freeze-dried and weighed as described for study 1. Blood sample collection and tissue excision and weighing were performed at the end of the experiment day without starvation as described for study 1.…”

Section: Animals and Dietsmentioning

confidence: 99%

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Spent turmeric reduces fat mass in rats fed a high-fat diet

et al. 2016

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Indigestible carbohydrates may improve obesity. Spent turmeric contains high levels of dietary fibre and resistant starch (RS), which have fermentation potential in vitro. We hypothesised that indigestible carbohydrates in spent turmeric might prevent obesity development. In the first study, rats were administered 10% turmeric powder (TP) or spent turmeric powder (STP) in a high-fat (HF) diet for 28 d. In the second study, rats were fed 10% STP in a HF diet with or without antibiotics for 15 d. In the third study, rats were treated with a STP-containing suspension. In study 1, the TP and STP diet increased the caecal short-chain fatty acid (SCFA) content compared to that of a control diet. The lower energy intake in the TP and STP group was strongly related to the decrease in visceral fat weight. In study 2, after caecal fermentation suppression with antibiotics, STP treatment decreased the visceral fat mass. In study 3, the plasma glucose levels and incremental area under the curve (AUC) after ingestion of a STP-containing suspension were lower than those after ingestion of suspension alone. These findings suggest the reduction of carbohydrate absorption during the gastrointestinal passage after TP and STP treatment. Our data indicate that the reduced obesity development in rats fed a HF diet may be attributed to the low metabolisable energy density of carbohydrates in the spent turmeric, independent of SCFA-mediated factors.

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Section: Discussionmentioning

confidence: 99%

Section: Animals and Dietsmentioning

confidence: 99%

Spent turmeric reduces fat mass in rats fed a high-fat diet

et al. 2016

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“…Elevated fibre content, including soyabean hulls, has been demonstrated to augment goblet cell abundances in the duodenum of piglets (Pascoal et al, 2015). Similarly, high fibre dietary components have been observed to stimulate goblet cell proliferation and activity in rodents (Ito et al, 2009;Hino et al, 2012, Hino et al, 2013. Such occurrences have also been associated with the increase in digesta viscosity by soluble NSPs (Piel et al, 2005).…”

Section: Intestinal Morphologymentioning

confidence: 99%