Non-discriminating flash pyrolysis and thermochemolysis of heavily contaminated sediments from the Hamilton Harbor (Canada)

Poerschmann, Juergen; Parsi, Z.; Górecki, Tadeusz

doi:10.1016/j.chroma.2007.08.051

Cited by 6 publications

(5 citation statements)

References 49 publications

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“…Nonetheless, several studies show that monocyclic aromatic compounds utilized by A. aromaticum EbN1 occur on a global scale at highly various concentrations in diverse environments. In surface sediments from the highly contaminated Randle Reef, Lake Ontario (Canada), concentrations of mixed m-and p-cresols ranged from 81.4 to 147.9 mol/kg of dry weight (42). In freshwater sediments of the Potomac River (USA), phenol and p-cresol were detected at concentrations of 0.98 nM and 92.9 nM, respectively (43).…”

Section: Discussionmentioning

confidence: 99%

Towards the Response Threshold for p -Hydroxyacetophenone in the Denitrifying Bacterium “Aromatoleum aromaticum” EbN1

Vagts

Scheve

Kant

et al. 2018

Appl Environ Microbiol

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The denitrifying betaproteobacterium "" EbN1 regulates the capacity to anaerobically degrade -ethylphenol (via-hydroxyacetophenone) with high substrate specificity. This process is mediated by the σ-dependent transcriptional regulator EtpR, which apparently recognizes both aromatic compounds, yielding congruent expression profiles. The responsiveness of this regulatory system was studied with -hydroxyacetophenone, which is more easily administered to cultures and traced analytically. Cultures of EbN1 were initially cultivated under nitrate-reducing conditions with a growth-limiting supply of benzoate, upon the complete depletion of which -hydroxyacetophenone was added at various concentrations (from 500 μM down to 0.1 nM). Depletion profiles of this aromatic substrate and presumptive effector were determined by highly sensitive micro-high-performance liquid chromatography (microHPLC). Irrespective of the added concentration of-hydroxyacetophenone, depletion commenced after less than 5 min and suggested a response threshold of below 10 nM. This approximation was corroborated by time-resolved transcript profiles (quantitative reverse transcription-PCR) of selected degradation and efflux relevant genes (e.g., , encoding a subunit of predicted-ethylphenol methylenehydroxylase) and narrowed down to a range of 10 to 1 nM. The most pronounced transcriptional response was observed, as expected, for genes located at the beginning of the two operon-like structures, related to catabolism (i.e., ) and potential efflux (i.e.,). Aromatic compounds are widespread microbial growth substrates with natural as well as anthropogenic sources, albeit with their concentrations and their bioavailabilities varying over several orders of magnitude. Even though degradation pathways and underlying regulatory systems have long been studied with aerobic and, to a lesser extent, with anaerobic bacteria, comparatively little is known about the effector concentration-dependent responsiveness. EbN1 is a model organism for the anaerobic degradation of aromatic compounds with the architecture of the catabolic network and its substrate-specific regulation having been intensively studied by means of differential proteogenomics. The present study aims at unraveling the minimal concentration of an aromatic growth substrate (-hydroxyacetophenone here) required to initiate gene expression for its degradation pathway and to learn in principle about the lower limit of catabolic responsiveness of an anaerobic degradation specialist.

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Section: Discussionmentioning

confidence: 99%

Towards the Response Threshold for p -Hydroxyacetophenone in the Denitrifying Bacterium “Aromatoleum aromaticum” EbN1

Vagts

Scheve

Kant

et al. 2018

Appl Environ Microbiol

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“…The non-discriminating thermochemolysis approach was due to the fact that high molecular weight fatty acids would have been discriminated in transfer from a conventional pyrolysis unit to the GC injection port [37]. In a follow-up study heavily contaminated sediments were analyzed by a similar non-discriminating pyrolysis approach where sample was introduced into a disposable stainless steel capillary column which was heated rapidly by passing an electric current [38]. This nondiscriminating thermochemolysis-TMAH was useful in screening anthropogenic contaminants and also differentiating between organic pollutants in the sediment and structures of different components of the polymeric humic sediment matrix.…”

Section: Devices Used For Thermochemolysismentioning

confidence: 99%

Recent applications in analytical thermochemolysis

Shadkami

Helleur

2010

Journal of Analytical and Applied Pyrolysis

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“…The locality, where this sample was collected, is characterized by human activity with the presence of restaurants and residences, and palmitonitrile may have resulted from some anthropogenic pyrolysis process. The butylmethacrylate found in the Holešovičky sample is often used in coating materials, and benzothiazole found in the Pankrác and Holešovičky dust is generated by combustion of sulphur containing fossil fuels (Poerschmann et al, 2008). Limonene is the major component of the oil extracted from the rinds of citrus fruits, and is Fig.…”

Section: Carbonaceous Matter Of Anthropogenic Originmentioning

confidence: 99%

“…PAHs found in the pyrograms (phenanthrene, methylphenathrene, naphtalene, anthracene, fluoranthene) indicate that they are of oil or coal origin, because crude oil and coals are alkylated homologues with smaller rings (originated from ancient plant material), whereas aromatic compounds derived from combustion of organic matter are predominantly parental with four to eight rings (Poerschmann et al, 2008). Relative abundances of selected compounds in the pyrolysis products are presented in Table 4.…”

Section: Pahs and Bcmentioning

confidence: 99%

Characterization of organic matter in dusts and fluvial sediments from exposed areas of downtown Prague, Czech Republic

Sýkorová¹,

Havelcová²,

Trejtnarová³

et al. 2009

International Journal of Coal Geology

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Non-discriminating flash pyrolysis and thermochemolysis of heavily contaminated sediments from the Hamilton Harbor (Canada)

Cited by 6 publications

References 49 publications

Towards the Response Threshold for p -Hydroxyacetophenone in the Denitrifying Bacterium “Aromatoleum aromaticum” EbN1

Towards the Response Threshold for p -Hydroxyacetophenone in the Denitrifying Bacterium “Aromatoleum aromaticum” EbN1

Recent applications in analytical thermochemolysis

Characterization of organic matter in dusts and fluvial sediments from exposed areas of downtown Prague, Czech Republic

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