Non-dinitrophenyl-binding immunoglobulin that bears a dominant idiotype (Id460) associated with antidinitrophenyl antibody is specific for an antigen on Pasteurella pneumotropica.

Marion, Tony N.; Dzierzak, Elaine; Lee, Hyunsoo; Adams, Ralph; Janeway, Charles A.

doi:10.1084/jem.159.1.221

Cited by 16 publications

(6 citation statements)

References 36 publications

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“…Antigeninduced anti-pneumococcal polysaccharide antibodies, for example, share idiotypic determinants with a large number of BALB/c myeioma proteins that bind phosphorylcholine (25). Similarly, antidinitrophenyl antibodies share idiotypic determinants with the myeloma protein MOPC 460 (27), and antibodies against a-l,3-a-l,6-dextran show idiotypes common to myeloma proteins J558, M 104, and U-102 (24,28).…”

Section: Gensmentioning

confidence: 99%

“…Another parallel between human and murine antibodies is the presence of the idiotype of an anti-DNA antibody in five antibodies that react with KlebsieUa antigens (NAE, WEA, ROS, MAY, and TO) (Table III). In the mouse, antiarsonate antibodies with the dominant arsonate-crossreactive idiotype-positive idiotype react with BruceUa abortus (cited in 12), and antibodies with the 460 idiotype (originally identified in antidinitrophenyl antibodies) react with Pasteu-reUa pneumotropica (27). The antibacterial properties of these antibodies, as well as those of the 16/6 family, may be a clue to their origin (29).…”

Section: Gensmentioning

confidence: 99%

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Immunochemical similarities between monoclonal antibacterial Waldenstrom's macroglobulins and monoclonal anti-DNA lupus autoantibodies.

Naparstek

Duggan

Schattner

et al. 1985

The Journal of Experimental Medicine

124

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Six monoclonal IgM from patients with Waldenstrom's macroglobulinemia that react with Klebsiella polysaccharides were tested for their ability to bind to nucleic acid antigens. One of the macroglobulins bound to the polynucleotide poly(G), and one bound to poly(G), poly(I), and single-stranded DNA. The reaction with the polynucleotides was specifically inhibited by the Klebsiella polysaccharide K30. A monoclonal lupus anti-DNA antibody (16/6) was found to react weakly with the Klebsiella polysaccharides K30 and K21. Five of the Waldenstrom macroglobulins shared an idiotypic determinant with the 16/6 anti-DNA antibody. The reaction between the macroglobulins and the antiidiotype serum was specifically inhibited by Klebsiella polysaccharides, an indication that the idiotypic marker was in the antigen-binding site of the macroglobulins. These results indicate the existence of widely dispersed conserved variable region genes that encode idiotypically related immunoglobulins with the capacity to bind to both bacterial polysaccharides and nucleic acids. Such genes can be expressed by patients with either Waldenstrom's macroglobulinemia or systemic lupus erythematosus.

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Section: Gensmentioning

confidence: 99%

Section: Gensmentioning

confidence: 99%

Immunochemical similarities between monoclonal antibacterial Waldenstrom's macroglobulins and monoclonal anti-DNA lupus autoantibodies.

Naparstek

Duggan

Schattner

et al. 1985

The Journal of Experimental Medicine

124

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“…Six were chosen for study: 2AB5 from the first spleen, Id16 from the second, and Id24, Id25, Id29, and Id30 from the third. Specificity for DNP or P. pneumotropica was tested as previously described (3,4).…”

Section: Methodsmentioning

confidence: 99%

“…

We have previously described an experimental system for studying the cellular and molecular basis of idiotype expression (1)(2)(3)(4)(5). BALB/c mice produce antidinitrophenyl (DNP) ~ antibody expressing a recurrent idiotypic determinant, Id-460.

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mentioning

confidence: 99%

Molecular characterization of antibodies bearing Id-460. II. Molecular basis for Id-460 expression.

Dzierzak¹,

Brodeur²,

Marion³

et al. 1985

The Journal of Experimental Medicine

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We have previously described an experimental system for studying the cellular and molecular basis of idiotype expression (1-5). BALB/c mice produce antidinitrophenyl (DNP) ~ antibody expressing a recurrent idiotypic determinant, Id-460. Two genes controlling the expression of this determinant map to the heavy and kappa chain variable regions (VH and V,j). Thus, the determinant is likely to be a combinatorial determinant involving both VH and V,l domains. 1d-460 is also found in normal serum, where much of it binds Pasteurella pneumotropica (P. pneumotropica), and none to DNP. Furthermore, P. pneumotropica immunization induces production of non-DNP-binding, Id-460 + antibodies. To understand the structural basis for this shared idiotypy, we have undertaken a molecular analysis of monoclonal Id-460 + antibodies. We have previously described the development of molecular probes for and the structure of the genes encoding the MOPC-460 (M460) protein, the prototypic DNP-binding antibody in the Id-460 system. We have also reported the structure of two Id-460 + hybridomas, LB8, which binds P. pneumotropica, and D35, which is DNP specific. These three proteins are encoded by closely related VH and V, gene segments, but differ in other gene segments (5). In the present experiments we use these probes to establish the molecular and genetic relatedness of other Id-460 + monoclonal antibodies in an attempt to determine the molecular basis of shared idiotypy by antibodies of differing antigen binding specificity.We have produced a panel of monoclonal antibodies selected for Id-460 expression. We obtained these Id-460 + monoclonal antibodies in two ways: by anti-Id-460 immunization or by DNP immunization. In this report, we demonstrate that all of the Id-460 ÷ hybridomas tested share immunoglobulin (lg) gene rearrangements and DNA sequence homologies for both Vu and VL with M460.

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“…Already in 1971 it was observed that idiotypic specificities could be expressed by immunoglobulins with different binding specificities ( 46 ). For instance, murine 2,4-dinitrophenyl (DNP) antibodies carry the Id460-idiotype which is also expressed on antibodies reacting with the mouse pathogen Pasteurella pneumotropica ( 47 ). A particular idiotope (B5 + ) of anti-dextran antibodies in BALB/c mice is expressed on a subset of antibodies from A/J mice that react with the hapten NIP ( 48 ).…”

Section: Principle Reactions In Idiotypic Regulationsmentioning

confidence: 99%

Immune Response Regulation by Antigen Receptors’ Clone-Specific Nonself Parts

Lemke

2018

Front. Immunol.

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Antigen determinants (epitopes) are recognized by the combining sites (paratopes) of B and T cell antigen receptors (BCR/TCR), which again express clone-specific epitopes (idiotopes) that can be recognized by BCR/TCR not only of genetically different donors but also within the autologous immune system. While xenogeneic and allogeneic anti-idiotypic BCR/TCR are broadly cross-reactive, only autologous anti-idiotypes are truly specific and of functional regulatory relevance within a particular immune system. Autologous BCR/TCR idiotopes are (a) somatically created at the third complementarity-determining regions, (b) through mutations introduced into BCRs during adaptive immune responses, and (c) through the conformational impact of both. As these idiotypic characters have no genomic counterparts they have to be regarded as antigen receptor-intrinsic nonself-portions. Although foreign, however, they are per se non-immunogenic, but in conjunction with immunogenicity- and adjuvanticity-providing antigen-induced immune responses, they induce abating regulatory idiotypic chain reactions. The dualistic nature of antigen receptors of seeing antigens (self and nonself alike) and being nonself at the same time has far reaching consequences for an understanding of the regulation of adaptive immune responses.

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Non-dinitrophenyl-binding immunoglobulin that bears a dominant idiotype (Id460) associated with antidinitrophenyl antibody is specific for an antigen on Pasteurella pneumotropica.

Cited by 16 publications

References 36 publications

Immunochemical similarities between monoclonal antibacterial Waldenstrom's macroglobulins and monoclonal anti-DNA lupus autoantibodies.

Immunochemical similarities between monoclonal antibacterial Waldenstrom's macroglobulins and monoclonal anti-DNA lupus autoantibodies.

Molecular characterization of antibodies bearing Id-460. II. Molecular basis for Id-460 expression.

Immune Response Regulation by Antigen Receptors’ Clone-Specific Nonself Parts

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