Non-deuterated 1H NMR Spectrometric Method for the Rapid and Direct Analysis of Alcohol Content in Hand Sanitizers

Abstract

In this study, a 1H nuclear magnetic resonance (NMR) method to quantify the alcohol content of alcohol-based hand sanitizers was developed and validated in non-deuterated solvent and unlocked mode conditions. Deionized water was used to prepare the solutions for the no-D method, which also matched the matrix of the samples. The generated integration areas were used to quantify the ethanol and isopropanol content of the sanitizer samples. The no-D method presented well-correlated calibration curves with a detection limit of 1.41% (v/v) and 2.61% (v/v) for isopropanol and ethanol, respectively. The experimental alcohol content in no-D conditions was compared to the results using the D2O method and was not significantly different (p > 0.05). Although there is no additional precautionary measure when handling D2O, non-deuterated solvents are more easily accessible, which makes a 1H NMR-based analysis more cost-effective.

Nanoplasmonic Decoration of Sacrificial Bacteria: Directing Interparticle Coupling Toward Multiplex Analysis of Antiseptic Alcohols

“…Until now, a wide variety of analytical techniques have been leveraged for analyzing the composition of alcohol-based sanitizers ranging from chromatographic methods such as high-performance liquid chromatography (HPLC), gas chromatography–flame ionization detection (GC-FID), and gas chromatography–mass spectroscopy (GC/MS) to various spectroscopic techniques like nuclear magnetic resonance (NMR), Raman, infrared (IR), and near-infrared (NIR) absorption spectrophotometry . However, these conventional approaches demand highly trained personnel, as well as expensive and bulky equipment, and they are frequently laborious and time-consuming.…”