Non-destructive mechanical assessment for optimization of 3D bioprinted soft tissue scaffolds

Godau, Brent; Stefanek, Evan; Gharaie, Sadaf Samimi; Amereh, Meitham; Pagan, Erik; Marvdashti, Zohreh; Libert-Scott, Eryn; Ahadian, Samad; Akbari, Mohsen

doi:10.1016/j.isci.2022.104251

Cited by 8 publications

(7 citation statements)

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“…Mechanisms of hydrolytic degradation have to be considered but secondary, as they mainly concern large microparticles (>250 µm), which are not manufactured in this work. The results obtained in this work demonstrated the efficacy of PLGA in allowing the release of AT101 in the physiological environment [27]. In particular, the microspheres manufactured not only allowed to increase the solubility of AT101 in an aqueous environment but also allowed to obtain a controlled release over time, better than previously developed systems [15].…”

Section: Discussionmentioning

confidence: 59%

“…To overcome this limitation, by means of the second approach (i.e. W/O method) a water-based stabilization bath was used in which the insolubility of the drug used is known [27]. This approach proved to be six times more effective than the previous one, with a high drug entrapment due to the fast diffusion of DCM molecules into the water stabilization bath, leaving the AT101 and PLGA molecules to quickly solidify and forming stable microparticles [37].…”

Section: Discussionmentioning

confidence: 99%

“…To characterize the fabricated samples, evaluate their geometry and size and verify the distribution of the PLGA microspheres within the polymeric matrix, the meshes were subjected to optical microscopy imaging (Zeiss Axio Observer, Oberkochen, Germany). Finally, in order to obtain a preliminary evaluation of the mechanical properties of the printed mesh, a non-destructive method was used as described in our previous work [27]. Storage modulus measurements were performed using an ElastoSens™ Bio2 (Rheolution, Montreal, Quebec, Canada), by printing and crosslinking the mesh design directly into the sample holder.…”

Section: Fabrication and Characterization Of 3d-bioprinted Alginate Meshmentioning

confidence: 99%

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A biopolymeric mesh enriched with PLGA microparticles loaded with AT101 for localized glioblastoma treatment

et al. 2023

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Current treatment strategies for glioblastoma (GBM) including surgical resection and adjuvant radio/chemotherapy result in a limited progression-free survival time of patients due to rapidly occurring tumor recurrences. The urgent need for more effective treatments has led to the development of different approaches for localized drug delivery systems (DDS) offering the advantages of reduced systemic side effects. A promising candidate for the treatment of GBMs is AT101, the R-(-)-enantiomer of gossypol due to its ability to induce apoptosis or trigger autophagic cells death in tumor cells. Here, we present an alginate-based drug-releasing mesh ladened with AT101-loaded PLGA microspheres (AT101-GlioMesh). The AT101-loaded PLGA microspheres were fabricated using an oil-in-water emulsion solvent evaporation method obtaining a high encapsulation efficiency. The drug-loaded microspheres enabled the release of AT101 over several days at the tumor site. The cytotoxic effect of the AT101-loaded mesh was evaluated using two different GBM cell lines. Strikingly, encapsulation of AT101 in PLGA-microparticles and subsequent embedding in GlioMesh resulted in a sustained delivery and more efficient cytotoxic effect of AT101 on both GBM cell lines. Thus, such a DDS holds promise for GBM therapy likely by preventing the development of tumor recurrences.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Fabrication and Characterization Of 3d-bioprinted Alginate Meshmentioning

confidence: 99%

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A biopolymeric mesh enriched with PLGA microparticles loaded with AT101 for localized glioblastoma treatment

et al. 2023

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“…Understanding the precise consistencies of the printing inks is crucial to unlocking the excellent potential of this technology. [36,37]…”

Section: Diw Multimaterials Hydrogel 3d Printingmentioning

confidence: 99%

Multimaterial Hydrogel 3D Printing

Imrie,

Jin

2023

Macro Materials & Eng

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In recent years, hydrogels have emerged as quintessential 3D printing materials. Coupled with their inherent printability, the unique mechanical properties, network structure, and biocompatibility of hydrogels make them ideal for a wide range of 3D printing applications. Also in recent years, the rise of multimaterial 3D printing, an additive manufacturing technology which involves at least two different materials in the fabrication process, has been witnessed. Advanced multimaterial 3D printing protocols have brought the field closer than ever before to a new industrial revolution. In this review, the use of hydrogels in multimaterial 3D printing is investigated. The review is sectioned by discussing three major multimaterial 3D printing methods: direct‐ink‐writing, vat‐switching, and coaxial extrusion. Subsections detail three common domains of multimaterial hydrogel 3D printing: bioprinting, 4D printing, and particle‐polymer composite printing. In the second part of the review, recent advancements in both multimaterial 3D printing hardware and hydrogel inks which are expected to steer the field in exciting new directions are explored. Finally, a case is made for coaxial extrusion and light‐responsive printers being the best choice for multimaterial hydrogel 3D printing in the long run, due to their gradient and greyscale printing capabilities.

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“…The porosity and rheological properties of these gels can be adjusted to control the release kinetics of drug delivery. Recently, shear-thinning hydrogels composed of silicate nanoparticles and hydrogels have been developed for various biomedical applications [ 11 , 12 , 16 , 17 ]. The shear-thinning properties of these nanocomposite gels rely on the edge-rim electrostatic interaction between the gel and nano silicate particles.…”

Section: Introductionmentioning

confidence: 99%

A Drug-Eluting Injectable NanoGel for Localized Delivery of Anticancer Drugs to Solid Tumors

Godau,

Samimi,

Seyfoori

et al. 2023

Pharmaceutics

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Systemically administered chemotherapy reduces the efficiency of the anticancer agent at the target tumor tissue and results in distributed drug to non-target organs, inducing negative side effects commonly associated with chemotherapy and necessitating repeated administration. Injectable hydrogels present themselves as a potential platform for non-invasive local delivery vehicles that can serve as a slow-releasing drug depot that fills tumor vasculature, tissue, or resection cavities. Herein, we have systematically formulated and tested an injectable shear-thinning hydrogel (STH) with a highly manipulable release profile for delivering doxorubicin, a common chemotherapeutic. By detailed characterization of the STH physical properties and degradation and release dynamics, we selected top candidates for testing in cancer models of increasing biomimicry. Two-dimensional cell culture, tumor-on-a-chip, and small animal models were used to demonstrate the high anticancer potential and reduced systemic toxicity of the STH that exhibits long-term (up to 80 days) doxorubicin release profiles for treatment of breast cancer and glioblastoma. The drug-loaded STH injected into tumor tissue was shown to increase overall survival in breast tumor- and glioblastoma-bearing animal models by 50% for 22 days and 25% for 52 days, respectively, showing high potential for localized, less frequent treatment of oncologic disease with reduced dosage requirements.

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Non-destructive mechanical assessment for optimization of 3D bioprinted soft tissue scaffolds

Cited by 8 publications

References 50 publications

A biopolymeric mesh enriched with PLGA microparticles loaded with AT101 for localized glioblastoma treatment

A biopolymeric mesh enriched with PLGA microparticles loaded with AT101 for localized glioblastoma treatment

Multimaterial Hydrogel 3D Printing

A Drug-Eluting Injectable NanoGel for Localized Delivery of Anticancer Drugs to Solid Tumors

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