Non-destructive electromechanical assessment (Arthro-BST) of human articular cartilage correlates with histological scores and biomechanical properties

Sim, Sotcheadt; Chevrier, Anik; Garon, M.; Quenneville, É.; Yaroshinsky, Alex; Hoemann, Caroline D.; Buschmann, Michael D.

doi:10.1016/j.joca.2014.08.008

Cited by 42 publications

(91 citation statements)

References 34 publications

(36 reference statements)

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“…Visual macroscopic evaluation has been subjected to many critiques involving poor inter‐observer agreement and incomplete validation to diagnose cartilage lesions . Mechanical and electromechanical properties have been recognized as accurate indicators of the functional properties of cartilage, provided by structure and composition . However, reliable instruments for practical evaluation of these properties over entire surfaces are currently lacking.…”

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confidence: 99%

“…Our research group has developed two unique approaches in the quantitative assessment of cartilage that overcome some limitations of other techniques. The first technique uses an arthroscopic probe to measure electromechanical properties of cartilage via compression‐induced streaming potentials . The second uses a multiaxial mechanical tester to perform automated spherical indentation and thickness measurements to map mechanical properties .…”

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Electromechanical probe and automated indentation maps are sensitive techniques in assessing early degenerated human articular cartilage

Sim

Chevrier

Garon

et al. 2016

Journal Orthopaedic Research

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Recent advances in the development of new drugs to halt or even reverse the progression of Osteoarthritis at an early-stage requires new tools to detect early degeneration of articular cartilage. We investigated the ability of an electromechanical probe and an automated indentation technique to characterize entire human articular surfaces for rapid non-destructive discrimination between early degenerated and healthy articular cartilage. Human cadaveric asymptomatic articular surfaces (four pairs of distal femurs and four pairs of tibial plateaus) were used. They were assessed ex vivo: macroscopically, electromechanically, (maps of the electromechanical quantitative parameter, QP, reflecting streaming potentials), mechanically (maps of the instantaneous modulus, IM), and through cartilage thickness. Osteochondral cores were also harvested from healthy and degenerated regions for histological assessment, biochemical analyses, and unconfined compression tests. The macroscopic visual assessment delimited three distinct regions on each articular surface: Region I was macroscopically degenerated, region II was macroscopically normal but adjacent to regions I and III was the remaining normal articular surface. Thus, each extracted core was assigned to one of the three regions. A mixed effect model revealed that only the QP (p < 0.0001) and IM (p < 0.0001) were able to statistically discriminate the three regions. Effect size was higher for QP and IM than other assessments, indicating greater sensitivity to distinguish early degeneration of cartilage. When considering the mapping feature of the QP and IM techniques, it also revealed bilateral symmetry in a moderately similar distribution pattern between bilateral joints. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:858-867, 2017.

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confidence: 99%

mentioning

confidence: 99%

Electromechanical probe and automated indentation maps are sensitive techniques in assessing early degenerated human articular cartilage

Sim

Chevrier

Garon

et al. 2016

Journal Orthopaedic Research

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“…A detailed description of this technique is reported in a recently published article. 31 Electromechanical Mapping. The Arthro-BST measures compression-induced streaming potentials in articular cartilage using an array of 37 gold microelectrodes lying on a semispherical indenter (effective radius = 3.2 mm, microelectrode density = 5.1 electrodes/mm 2 , microelectrode effective diameter = 75 µm).…”

Section: Electromechanicsmentioning

confidence: 99%

“…The Arthro-BST measures compression-induced streaming potentials in articular cartilage using an array of 37 gold microelectrodes lying on a semispherical indenter (effective radius = 3.2 mm, microelectrode density = 5.1 electrodes/mm 2 , microelectrode effective diameter = 75 µm). 31 The device's quantitative parameter (QP, A.U.) reflects the amplitude of indentation (number of microelectrodes in contact with cartilage) reached when the sum of the measured electric potential is 100 mV (thus inversely related to the electromechanical function).…”

Section: Electromechanicsmentioning

confidence: 99%

Bilayer Implants

et al. 2016

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Objective. To compare the regenerative capacity of 2 distinct bilayer implants for the restoration of osteochondral defects in a preliminary sheep model. Methods. Critical sized osteochondral defects were treated with a novel biomimetic poly-ε-caprolactone (PCL) implant (Treatment No. 2; n = 6) or a combination of Chondro-Gide and Orthoss (Treatment No. 1; n = 6). At 19 months postoperation, repair tissue (n = 5 each) was analyzed for histology and biochemistry. Electromechanical mappings (Arthro-BST) were performed ex vivo. Results. Histological scores, electromechanical quantitative parameter values, dsDNA and sGAG contents measured at the repair sites were statistically lower than those obtained from the contralateral surfaces. Electromechanical mappings and higher dsDNA and sGAG/weight levels indicated better regeneration for Treatment No. 1. However, these differences were not significant. For both treatments, Arthro-BST revealed early signs of degeneration of the cartilage surrounding the repair site. The International Cartilage Repair Society II histological scores of the repair tissue were significantly higher for Treatment No. 1 (10.3 ± 0.38 SE) compared to Treatment No. 2 (8.7 ± 0.45 SE). The parameters cell morphology and vascularization scored highest whereas tidemark formation scored the lowest. Conclusion. There was cell infiltration and regeneration of bone and cartilage. However, repair was incomplete and fibrocartilaginous. There were no significant differences in the quality of regeneration between the treatments except in some histological scoring categories. The results from Arthro-BST measurements were comparable to traditional invasive/destructive methods of measuring quality of cartilage repair.

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“…To overcome limitations of traditional microindentation testing for biologic tissues, we employed an indentation system capable of collecting indentation mapping data across irregularly shaped surfaces to improve existing mechanical testing approaches for laryngeal tissues. This technique successfully measured mechanical properties of irregular geometric surfaces such as articular cartilage . For the purpose of this investigation and to collect valuable normative data, we chose to compare intact, excised hemilarynges from three common animal laryngeal models: swine, leporine, and canine.…”

Section: Introductionmentioning

confidence: 99%

Automated Indentation Mapping of Vocal Fold Structure and Cover Properties Across Species

et al. 2018

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Objectives/Hypothesis Various animal models have been employed to investigate vocal fold (VF) and phonatory function. However, biomechanical testing techniques to characterize vocal fold structural properties vary and have not compared critical properties across species. We adapted a non-destructive, automated indentation mapping technique to simultaneously quantify VF structural properties (VF cover layer and intact VF) in commonly used species based on the hypothesis that VF biomechanical properties are largely preserved across species. Study Design Ex vivo Methods Canine, leporine, and swine larynges (n=4 each) were sagittally bisected, measured, and subjected to normal indentation mapping (indentation at 0.3mm; 1.2mm/s) with a 2mm spherical indenter to quantify normal force along the VF cover layer, structural stiffness, and displacement at 0.8mN; 2-D maps of the free VF edge through the conus elasticus were created for these characterizations. Results Structural stiffness was 7.79gf/mm (0.15–74.55) for leporine, 2.48gf/mm (0.20–41.75) for canine, and 1.45gf (0.56–4.56) for swine. For each species, the lowest values were along the free VF edge (mean ± SD; leporine: 0.40±0.21gf/mm, canine: 1.14±0.49gf/mm, swine: 0.89±0.28gf/mm). Similar results were obtained for the cover layer normal force at 0.3mm. On the free VF edge, mean (SD) displacement at 0.08gf was 0.14mm (0.05) in leporine, 0.11mm (0.03) in canine, and 0.10mm (0.02) in swine. Conclusions Automated indentation mapping yielded reproducible biomechanical property measurement of the VF cover and intact VF. Divergent VF structural properties across canine, swine, and leporine species were observed. Level of Evidence N/A

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Non-destructive electromechanical assessment (Arthro-BST) of human articular cartilage correlates with histological scores and biomechanical properties

Abstract: Non-destructive electromechanical QP measurements correlate strongly with histological scores and biomechanical parameters providing a rapid and reliable assessment of articular cartilage quality.

Cited by 42 publications

References 34 publications

Electromechanical probe and automated indentation maps are sensitive techniques in assessing early degenerated human articular cartilage

Electromechanical probe and automated indentation maps are sensitive techniques in assessing early degenerated human articular cartilage

Bilayer Implants

Automated Indentation Mapping of Vocal Fold Structure and Cover Properties Across Species

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