Non-cytotoxic Thymus capitata extracts prevent Bovine herpesvirus-1 infection in cell cultures

Boubaker¿Elandalousi, Ramzi; Mekni-Toujani, Marwa; Kaabi, Belhassen; Larbi, Imen; Diouani, Mohamed Fethi; Gharbi, Mohamed; Akkari, Hafidh; B¿chir, Fatma; Ghram, Abdeljelil

doi:10.1186/preaccept-1942837218128489

Cited by 4 publications

(3 citation statements)

References 20 publications

(33 reference statements)

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“…The relative cell viability rate was determined for each concentration based on the following formula: (OD 450 drug)/(OD 450 control) × 100%. APS concentrations below the 50% cytostatic concentration (CC 50 ) were defined as non-toxic concentrations [16].…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Astragalus polysaccharides inhibit avian infectious bronchitis virus infection by regulating viral replication

Zhang

Liu

et al. 2018

Microbial Pathogenesis

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The avian coronavirus causes infectious bronchitis (IB), which is one of the most serious diseases affecting the avian industry worldwide. However, there are no effective strategies for controlling the IB virus (IBV) at present. Therefore, development of novel antiviral treatment strategies is urgently required. As reported, astragalus polysaccharides (APS) have potential antiviral effects against several viruses; however, the antiviral effect of APS against IBV remains unclear. In this study, we explored whether APS had the potential to inhibit IBV infectionby utilizing several in vitro experimental approaches. To this end, the effect of APS on the replication of IBV was examined in chicken embryo kidney (CEK) cells. Viral titers were calculated by using the plaque formation assay, and the cytotoxicity of APS was tested by utilizing a Cell Counting Kit-8 assay. The expression of viral mRNA and cytokine (IL-1β, IL-6, IL-8 and TNF-α) mRNA transcripts was determined by real-time quantitative RT-PCR(qRT-PCR). IBV titers in infected CEK cells treated with APS were significantly reduced in a dose-dependent manner, indicating that APS inhibited IBV replication in vitro. We also found that the decreased viral replication after APS treatment was associated with reduced mRNA levels of the cytokines IL-1B, IL-6, IL-8 and TNF-α. In conclusion, these results suggest that APS exhibit antiviral activities against IBV and it may represent a potential therapeutic agent for inhibiting the replication of IBV.

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Section: Cytotoxicity Assaymentioning

confidence: 99%

Astragalus polysaccharides inhibit avian infectious bronchitis virus infection by regulating viral replication

Zhang

Liu

et al. 2018

Microbial Pathogenesis

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“…The ethanolic and aqueous extracts (EE and AE) were prepared as previously described (Boubaker-Elandalousi et al, 2014). The essential oil (EO) was prepared by dissolving 100 g of dried plant material in 1 L of distilled water and then submitted to microwave-assisted hydro-distillation at 40°C during 4 hr, in a Clevenger type apparatus.…”

Section: Extracts Preparationmentioning

confidence: 99%

Inhibition of HSV‐2 infection by pure compounds from Thymus capitatus extract in vitro

Toujani

Rittá

Civra

et al. 2018

Phytotherapy Research

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Thymus capitatus represents 1 of the 5 Tunisian species of the genus Thymus, which has long‐standing use for flavouring and preserving several food products. Its constituents have been reported to endow antimicrobial properties, but little is known about their antiviral activities. The aim of this study was to examine the antiviral activity of pure compounds from the most bioactive inhibitory T. capitatus extract in vitro against herpes simplex virus Type 2 (HSV‐2) infection and to identify their mechanism of action. Either the extracts or the essential oil exert inhibitory activity against HSV‐2 infection, with the ethanolic extract showing the lowest EC50 value (2.3 μg/ml). Three pure compounds were then isolated from the ethanolic extract and investigated for their antiviral activity. β‐sitosterol showed the most favourable selectivity index and both cinnamaldehyde and carvacrol exerted moderate antiviral effect. Investigation of the mechanism of action revealed that all three compounds directly inactivated the infectivity of the virus particles. These findings suggest the use of T. capitatus ethanolic extract as source of anti‐HSV‐2 pure compounds and warrant further studies to evaluate their therapeutic potential.

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“…In addition, PCR coupled with MS to identify viral nucleic acids from cerebrospinal fluid samples has proven to be a valuable tool for early diagnosis of neurological disorders correlated with infection by neurotropic viruses such as HSV-1 and VZV [97]. MS facilitated the analysis of the chemical composition of Thymus capitata extracts, which have a potential noncytotoxic antiviral effect against BHV-1 [98].…”

Section: Nonproteomic Use Of Ms In Alpha Herpesvirus Researchmentioning

confidence: 99%

Investigating the biology of alpha herpesviruses with MS‐based proteomics

et al. 2015

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Viruses are intracellular parasites that can only replicate and spread in cells of susceptible hosts. Alpha herpesviruses (α-HVs) contain double stranded DNA genomes of at least 120 kb, encoding for 70 or more genes. The viral genome is contained in an icosahedral capsid that is surrounded by a proteinaceous tegument layer and a lipid envelope. Infection starts in epithelial cells and spreads to the peripheral nervous system (PNS). In the natural host, α-HVs establish a chronic latent infection that can be reactivated, rarely spread to the central nervous system (CNS). In the non-natural host, viral infection will in most cases spread to the CNS with often fatal outcome. The host response plays a crucial role in the outcome of viral infection. α-HVs do not encode all the genes required for viral replication and spread. They need a variety of host gene products including RNA polymerase, ribosomes, dynein, and kinesin. As a result, the infected cell is dramatically different from the uninfected cell revealing a complex and dynamic interplay of viral and host components required to complete the virus life cycle. In this review, we describe the pivotal contribution of mass spectrometry-based proteomics (MSBP) studies over the past 15 years to understanding the complicated life cycle and pathogenesis of four α-HV species from the alphaherpesvirinae subfamily: Herpes simplex virus-1 (HSV-1), varicella zoster virus (VZV), pseudorabies virus (PRV) and bovine herpes virus (BHV-1). We describe the viral proteome dynamics during host infection and the host proteomic response to counteract such pathogens.

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Non-cytotoxic Thymus capitata extracts prevent Bovine herpesvirus-1 infection in cell cultures

Cited by 4 publications

References 20 publications

Astragalus polysaccharides inhibit avian infectious bronchitis virus infection by regulating viral replication

Astragalus polysaccharides inhibit avian infectious bronchitis virus infection by regulating viral replication

Inhibition of HSV‐2 infection by pure compounds from Thymus capitatus extract in vitro

Investigating the biology of alpha herpesviruses with MS‐based proteomics

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