Non-covalent functionalization of single-walled carbon nanotubes with modified polyethyleneimines for efficient gene delivery

Behnam, Behzad; Shier, W. Thomas; Nia, Azadeh Hashem; Abnous, Khalil; Ramezani, Mohammad

doi:10.1016/j.ijpharm.2013.06.057

Cited by 76 publications

(33 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Under optimal conditions, the plasmid with EGFP (pEGFP)-N1 transfection efficiency of MWCNTs-CS-FA-NPs was 1.5-fold higher than that of non-functionalized MWCNTs; they did not decrease cell viability up to a concentration of 250 μg mL -1 . Behnam et al 68 functionalized SWCNTs via non-covalent bonds with various types of PEIs. All PEI-functionalized CNTs showed good stability and dispersion ability in aqueous solution.…”

Section: Son Et Almentioning

confidence: 99%

Carbon nanotubes as cancer therapeutic carriers and mediators

Son¹,

Hong²,

Lee³

2016

IJN

213

106

View full text Add to dashboard Cite

Carbon nanotubes (CNTs) have received increasing attention in biomedical fields because of their unique structures and properties, including high aspect ratios, large surface areas, rich surface chemical functionalities, and size stability on the nanoscale. Particularly, they are attractive as carriers and mediators for cancer therapy. Through appropriate functionalization, CNTs have been used as nanocarriers for anticancer drugs including doxorubicin, camptothecin, carboplatin, cisplatin, paclitaxel, Pt(II), and Pt(IV), and genes including plasmid DNA, small-interfering RNA, oligonucleotides, and RNA/DNA aptamers. CNTs can also deliver proteins and immunotherapy components. Using combinations of light energy, they have also been applied as mediators for photothermal therapy and photodynamic therapy to directly destroy cancer cells without severely damaging normal tissue. If limitations such as a long-term cytotoxicity in the body, lack of size uniformity during the synthetic process, loading deviations for drug–CNT complexes, and release controllability at the target point are overcome, CNTs will become one of the strongest tools that are available for various other biomedical fields as well as for cancer therapy.

show abstract

Section: Son Et Almentioning

confidence: 99%

Carbon nanotubes as cancer therapeutic carriers and mediators

Son¹,

Hong²,

Lee³

2016

IJN

213

106

View full text Add to dashboard Cite

show abstract

“…Previously, PEG was successfully used for stabilizing CNTs through a steric-based mechanism when it is bonded to hydrophobic moieties such as linear alkyl chains, which adsorb on the CNT preserved graphitic sheets and thus leave the hydrophilic PEO residue exposed to the aqueous media [37][38][39][40][41] . However, to the best of the authors´ knowledge, the colloidal stabilization of CNTs through repulsive depletion forces manifested in the presence of PEG has not been reported.…”

Section: Nanocarbons Morphological and Structural Characterization Tmentioning

confidence: 99%

Mechanisms of Colloidal Stabilization of Oxidized Nanocarbons in the Presence of Polymers: Obtaining Highly Stable Colloids in Physiological Media

Padovani¹,

Petry²,

Holanda³

et al. 2015

J. Phys. Chem. C

View full text Add to dashboard Cite

There are successful protocols for dispersing carbon nanotubes and graphene oxide in physiological media by using biocompatible polymers, which enable their use in nanomedicine. However, there is not a clear understanding regarding the mechanisms of the colloidal stabilization manifested (i.e., electric, steric or electrosteric or through depletion forces). Here we show that the manifestation of a particular mechanism of stabilization for oxidized carbon nanotubes (CNTs) and graphene oxide (GO) in the presence of Pluronic F-127 (PF127) and short-and long-chain polyethylene glycol (PEG 1,500 or 35,000 respectively) depends on a proper matching between the nanocarbon morphology and the polymer chain length, chemical structure and concentration. The high aspect ratio one dimensional morphology of CNTs enables an initial steric and electrosteric stabilization through the nanotube wrapping (i.e., adsorption) by PF127 present in low concentrations (<0.1%). Depletion stabilization for CNTs manifests when PF127 is present in high concentrations (≥1.0%), thus enabling the formation of highly stable CNT colloids even in a 0.9% NaCl saline solution. This depletion stabilization depends little on the CNT structure (i.e., single-or multi-wall), surface charge (i.e., zeta potential), oxidation and carboxylation degree or the nanotube length. On the other hand, large-sized sheets of GO could be colloidally stabilized in NaCl 0.9% only in the presence of PEG 35,000 through repulsive depletion forces, whose manifestation occurs with a polymer concentration threshold of 5.0 wt%. Comparatively, in a physiological saline solution, PF127 is able to colloidally stabilize CNTs to a much large extent than PEG 35,000 stabilizes the large GO sheets.

show abstract

“…These results suggest that the oxidation introduces polar groups (oxygen-containing groups) on the CNTs surface, which create the electrostatic stability required for a long time stable dispersion in polar solvents [14,20,26,27]. Presumably, electrostatic repulsion between the surface charges of the functionalized nanotube surface contributes to its stability [29]. The dispersibility of CNT-Am in ethanol and water was classified as sedimented, as shown in Fig.…”

Section: Resultsmentioning

confidence: 91%

“…Band at approximately 3445 cm −1 was observed in the spectrum of the CNT-H sample (Fig. 2a), that may be assigned to O H stretching vibrations [27,29]. The CNT-H spectrum also showed band at 1630 cm −1 , which was assigned to the C C stretching.…”

Section: Resultsmentioning

confidence: 91%