Non-contact small animal fluorescence imaging system for simultaneous multi-directional angular-dependent data acquisition

Lee, Jong Hwan; Kim, Hyun Keol; Chandhanayingyong, Chandhanarat; Lee, Francis Young‐In; Hielscher, Andreas H.

doi:10.1364/boe.5.002301

Cited by 14 publications

(8 citation statements)

References 23 publications

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“…An in-depth broad review of commercial scanners has been provided by Leblond et al [23]. Regarding research prototypes, most acquire CW data [52][53][54][55][56][57][58][59][60] and a few are FD systems [56,[61][62][63][64]. Interestingly, a study comparing CW and FD systems and image reconstruction suggested that FD measurements may result in superior images but at much higher costs and efforts as regards instrumentation and computational resources [65].…”

Section: Journal Of Spectroscopymentioning

confidence: 99%

Prospects on Time-Domain Diffuse Optical Tomography Based on Time-Correlated Single Photon Counting for Small Animal Imaging

Bérubé-Lauzière

Crotti

Boucher

et al. 2016

Journal of Spectroscopy

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This paper discusses instrumentation based on multiview parallel high temporal resolution (<50 ps) time-domain (TD) measurements for diffuse optical tomography (DOT) and a prospective view on the steps to undertake as regards such instrumentation to make TD-DOT a viable technology for small animal molecular imaging. TD measurements provide information-richest data, and we briefly review the interaction of light with biological tissues to provide an understanding of this. This data richness is yet to be exploited to its full potential to increase the spatial resolution of DOT imaging and to allow probing, via the fluorescence lifetime, tissue biochemical parameters, and processes that are otherwise not accessible in fluorescence DOT. TD data acquisition time is, however, the main factor that currently compromises the viability of TD-DOT. Current high temporal resolution TD-DOT scanners simply do not integrate sufficient detection channels. Based on our past experience in developing TD-DOT instrumentation, we review and discuss promising technologies to overcome this difficulty. These are single photon avalanche diode (SPAD) detectors and fully parallel highly integrated electronics for time-correlated single photon counting (TCSPC). We present experimental results obtained with such technologies demonstrating the feasibility of next-generation multiview TD-DOT therewith.

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Section: Journal Of Spectroscopymentioning

confidence: 99%

Prospects on Time-Domain Diffuse Optical Tomography Based on Time-Correlated Single Photon Counting for Small Animal Imaging

Bérubé-Lauzière

Crotti

Boucher

et al. 2016

Journal of Spectroscopy

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“…However, due to the strong scattering nature of optical photons in deep tissues and a limited number of measurements, the inverse problem of FMT is ill-posed and underdetermined, which results in low spatial resolution in FMT imaging, in particular for targets in deep turbid media. 2 Many approaches have been proposed to improve the FMT image quality, including multispectral wavelengths for both excitation and emission wavelengths, different illumination patterns, 12,13 a large number of FMT measurements by using charge-coupled device(CCD) cameras, [14][15][16][17][18] and improved FMT reconstruction algorithms--especially the sparse enhancement FMT reconstruction for the sparse FMT targets [19][20][21][22][23][24] . A region reconstruction method implemented with level set method was also introduced to improve the FMT image reconstruction.…”

Section: Introductionmentioning

confidence: 99%

Anatomical image-guided fluorescence molecular tomography reconstruction using kernel method

et al. 2017

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Abstract. Fluorescence molecular tomography (FMT) is an important in vivo imaging modality to visualize physiological and pathological processes in small animals. However, FMT reconstruction is ill-posed and illconditioned due to strong optical scattering in deep tissues, which results in poor spatial resolution. It is well known that FMT image quality can be improved substantially by applying the structural guidance in the FMT reconstruction. In this paper, a new approach to introducing anatomical information into the FMT reconstruction is presented using the kernel method. In contrast to conventional methods that incorporate anatomical information with a Laplacian-type regularization matrix, the proposed method introduces the anatomical guidance into the projection model of FMT. The primary advantage of the proposed method is that it does not require segmentation of targets in the anatomical images. Numerical simulations and phantom experiments have been performed to demonstrate the proposed approach's feasibility. Numerical simulation results indicate that the proposed kernel method can separate two FMT targets with an edge-to-edge distance of 1 mm and is robust to false positive guidance and inhomogeneity in the anatomical image. For the phantom experiments with two FMT targets, the kernel method has reconstructed both targets successfully, which further validates the proposed kernel method. We have compared the proposed kernel method with the soft prior method thoroughly and found that the kernel method without target segmentation is able to achieve similar anatomical guided results as the soft prior method.

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“…For reflection mode, laser excitation and measurements are performed at the same side, 7 and it also lacks the information on the opposite side. Recently scholars have developed whole surface measurement FMT imaging systems 1,8,9 , in order to detect emission photons at most possible positions. The whole surface measurement mode can obtain more measurements than the half surface measurement modes, however, it might suffer from dynamic range problem.…”

Section: Introductionmentioning

confidence: 99%

Optimization and performance evaluation of a conical mirror based fluorescence molecular tomography imaging system

et al. 2016

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We performed numerical simulations and phantom experiments with a conical mirror based fluorescence molecular tomography (FMT) imaging system to optimize its performance. With phantom experiments, we have compared three measurement modes in FMT: the whole surface measurement mode, the transmission mode, and the reflection mode. Our results indicated that the whole surface measurement mode performed the best. Then, we applied two different neutral density (ND) filters to improve the measurement's dynamic range. The benefits from ND filters are not as much as predicted. Finally, with numerical simulations, we have compared two laser excitation patterns: line and point. With the same excitation position number, we found that the line laser excitation had slightly better FMT reconstruction results than the point laser excitation. In the future, we will implement Monte Carlo ray tracing simulations to calculate multiple reflection photons, and create a look-up table accordingly for calibration.

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Non-contact small animal fluorescence imaging system for simultaneous multi-directional angular-dependent data acquisition

Cited by 14 publications

References 23 publications

Prospects on Time-Domain Diffuse Optical Tomography Based on Time-Correlated Single Photon Counting for Small Animal Imaging

Prospects on Time-Domain Diffuse Optical Tomography Based on Time-Correlated Single Photon Counting for Small Animal Imaging

Anatomical image-guided fluorescence molecular tomography reconstruction using kernel method

Optimization and performance evaluation of a conical mirror based fluorescence molecular tomography imaging system

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