Non-contact Raman spectroscopic pH measurement of cerebrospinal fluid: in vivo rat and perimortem swine models

Fillioe, Seth; Bishop, Kyle Kelly; Satalin, Joshua; Blair, Sarah; Nieman, Gary F.; Jannini, Alexander Vincent; Kim, Jon Jong In; McDonough, Richard; Ortiz, Steven; Goodisman, Jerry; Hasenwinkel, Julie M.; Chaiken, J.

doi:10.1117/12.2546415

Cited by 2 publications

(6 citation statements)

References 5 publications

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“…Current efforts are now focused on including glucose in the PVOH platform. Table 1 includes the vital signs/variables for which there is preliminary published data [38][39][40][41][42][43] that could result from a new PVOH configuration along with technical comments of feasibility and differences from currently used technologies. Elastic scattering and fluorescence/Raman activity of a tissue, turbid medium, object, or material can be used for imaging fingertips 10 or spinal cords 42 and other applications with a PVOH.…”

Section: Additional Potential Modifications In the Covid Pandemicmentioning

confidence: 99%

“…Recently PVOH with Raman was able to image and measure the pH of cerebrospinal fluid in vivo and perimortem in rat and pig models. 42,43 In summary, the journey that began with a search for an approach to noninvasive blood glucose monitoring technologies has now come full circle to providing the raw data to stimulate an era of noninvasive analytics that may benefit not only those with diabetes but many others as well.…”

Section: Additional Potential Modifications In the Covid Pandemicmentioning

confidence: 99%

“…Recently PVOH with Raman was able to image and measure the pH of cerebrospinal fluid in vivo and perimortem in rat and pig models. 42,43…”

Section: Additional Potential Modifications In the Covid Pandemicmentioning

confidence: 99%

“…Vital Signs/Variables for Which There Is Preliminary Published Data[38][39][40][41][42][43] That Could Result From a New PVOH Configuration Along With Technical Comments of Feasibility and Differences From Currently Used Technologies.…”

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confidence: 99%

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Noninvasive Blood and Tissue Analysis: Raman Spectroscopy, One Perspective for Monitoring of Glucose and Beyond

Chaiken

2020

J Diabetes Sci Technol

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Noninvasive in vivo blood and tissue analysis remains a challenge to medical technology epitomized by the ongoing quest to replace fingerstick self-monitoring of blood glucose. Recent developments warrant comment on near-term prospects for using Raman spectroscopy to meet that challenge. These developments combined with 20 years of experimentation with noninvasive blood and tissue analysis suggest that it may be possible and practical to perform noninvasive in vivo glucose analysis with improvements in (1) the enabling technologies for making Raman measurements and (2) an underlying anatomical–physiological model of how in vivo spectroscopic measurements are made and interpreted. We review the substantial progress made toward meeting the challenge and the personal, public health, and economic implications of these ongoing efforts.

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Section: Additional Potential Modifications In the Covid Pandemicmentioning

confidence: 99%

Section: Additional Potential Modifications In the Covid Pandemicmentioning

confidence: 99%

“…Recently PVOH with Raman was able to image and measure the pH of cerebrospinal fluid in vivo and perimortem in rat and pig models. 42,43…”

Section: Additional Potential Modifications In the Covid Pandemicmentioning

confidence: 99%

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confidence: 99%

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Noninvasive Blood and Tissue Analysis: Raman Spectroscopy, One Perspective for Monitoring of Glucose and Beyond

Chaiken

2020

J Diabetes Sci Technol

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“…The PVOH and Raman CSF results have been previously published 7,8,9 and patents have been obtained 10,11 in addition to the in vivo PVOH and FRD patents. Since the algorithms have been described in detail in various publications, in this paper we present a very brief introduction to PVOH/FRD before describing the addition of Raman based pH mapping and then implications for diagnosing and possibly treating spinal cord injury.…”

Section: Introductionmentioning

confidence: 99%

PVOH and spontaneous Raman-based images of spinal cord in the immediate aftermath of localized contusive injury obtained noninvasively and in vivo in a rat model using remitted light from a single spatially scanned laser

Fillioe

Bishop

Jannini

et al. 2023

Medical Imaging 2023: Biomedical Applications in Molecular, Structural, and Functional Imaging

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We produce 2 kinds of images of spinal cord in the immediate aftermath of a localized contusive injury, noninvasively and in vivo in a rat model using remitted light from a single spatially scanned 830 nm laser. Two different processing algorithms/modalities were employed 1) the PVOH algorithm producing images based on the turbidity of the spinal cord tissue which includes the cerebrospinal fluid (CSF) and 2) Raman spectroscopy which allows images based on pH. Whereas PVOH requires relatively little data processing e.g., no separation of Raman and fluorescence emission for implementation, quantitative use of spontaneous Raman spectra to calculate e.g., pH requires careful separation and accounting for the presence of underlying fluorescence. We utilize an unbiased procedure i.e., no assumptions are made, that an unsupervised machine can execute based on the inherent differences in line widths for pure vibrational and vibronic transitions in fluid media, and the overall effect of heterogenous spectral broadening at physiological temperature. The fundamentally stronger signal to noise ratio for PVOH imaging allows much greater spatial resolution for the same collection times needed to construct the Raman based pH images. This proof of principle study is consistent with a hypothesis that fast localized change in the CSF pH, induced by the mechanical disruption of tissues during the injury, disrupts the delicate balance of chemical factors stabilizing the CSF phase stability. We suggest that this combination of new methodologies for real-time imaging of chemical and physical changes in spinal cords and other tissues could have many uses.

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Non-contact Raman spectroscopic pH measurement of cerebrospinal fluid: in vivo rat and perimortem swine models

Cited by 2 publications

References 5 publications

Noninvasive Blood and Tissue Analysis: Raman Spectroscopy, One Perspective for Monitoring of Glucose and Beyond

Noninvasive Blood and Tissue Analysis: Raman Spectroscopy, One Perspective for Monitoring of Glucose and Beyond

PVOH and spontaneous Raman-based images of spinal cord in the immediate aftermath of localized contusive injury obtained noninvasively and in vivo in a rat model using remitted light from a single spatially scanned laser

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