Non-contact, in-vivo, functional, and structural ophthalmic imaging using multimodal photoacoustic remote sensing (PARS) microscopy and swept source optical coherence tomography (SS-OCT)

Abstract

Early diagnosis of ocular diseases improves the understanding of pathophysiology and aids in accurate monitoring and effective treatment. Advanced, multimodal ocular imaging platforms play a crucial role in visualization of ocular components and provide clinicians with a valuable tool for evaluating various eye diseases. Here, for the first time we present a non-contact, multiwavelength photoacoustic remote sensing (PARS) microscopy and swept-source optical coherence tomography (SS-OCT) for in-vivo functional and structural imaging of the eye. The system provides complementary imaging contrasts of optical absorption and optical scattering, and is used for non-contact, in-vivo imaging of murine eye. Results of vasculature and structural imaging as well as melanin content in the retinal pigment epithelium layer are presented. Multiwavelength PARS microscopy using Stimulated Raman Scattering is applied to enable in-vivo, non-contact oxygen saturation estimation in the ocular tissue. The reported work may be a major step towards clinical translation of ophthalmic technologies and has the potential to advance the diagnosis and treatment of ocular diseases.

Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography

“…We then merge the co-registered PARS and OCT data to provide three-dimensional, histological, and structural tissue visualizations. While a combined PARS and OCT system has previously been proposed for ocular and vascular imaging 35,36 , this is the first such system for histopathology. Applying the SS-OCT in resected Rattus tissues we recover volumetric images containing adipocytes, ducts, fascia layers and other tissue features.…”