Non-contact helium-based plasma for delivery of DNA vaccines

Connolly, Richard J.; Chapman, Taryn; Hoff, Andrew; Kutzler, Michele A.; Jaroszeski, Mark J.; Ugen, Kenneth E.

doi:10.4161/hv.21624

Cited by 18 publications

(15 citation statements)

References 31 publications

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“…In the study of Conolly et al . , the authors observed a potent transfer of plasmids into cells in vivo, but concluded a nontypical poration process not following the laws of electroporation. In sum, it can be deduced that plasma filaments (DBD filaments with spread velocity of 5 × 10 8 cm/s, electron density of 10 15 per cm 3 and electron temperature of 3–5 eV) contacting cell surfaces can promote plasma access into the upper cell layer .…”

Section: Discussionmentioning

confidence: 99%

Comparison between cold plasma, electrochemotherapy and combined therapy in a melanoma mouse model

Daeschlein

Scholz

Lutze

et al. 2013

Experimental Dermatology

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The study was undertaken to compare antitumor efficacy of electrochemotherapy (ECT) with cold plasma therapy (CP) in a melanoma mouse model. After melanoma implantation into the flank of C57BL/6N mice, CP by two different plasma sources (APPJ and DBD) was applied directly to the tumor surface. ECT was performed with bleomycin intravenously at a field strength of 1000 V/cm without or combined with CP. Primary endpoints were tumor growth acceleration (TGA), daily volume progression (DVP) and survival after treatment. Both plasma sources as single treatment showed a significant TGA delay, which proved less effective than ECT. CP (APPJ) combined with ECT (ECJ) significantly improved per cent mouse survival, with significant superiority compared with ECT. Plasma therapy alone albeit less effective seems a potential alternative to ECT in patients with melanoma and can be applied manifold in a session without general anaesthesia. Accordingly, CP alone and combined with ECT may serve as new option in palliative skin melanoma therapy.

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Section: Discussionmentioning

confidence: 99%

Comparison between cold plasma, electrochemotherapy and combined therapy in a melanoma mouse model

Daeschlein

Scholz

Lutze

et al. 2013

Experimental Dermatology

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“…A DNA vaccine targeting an HIV‐1 protein was administered to mice intradermally, followed by local NTP application at the site of injection. [ 99 ] The vaccine was selected, because it only produces weak immune responses. An enhanced cellular and humoral (antibody) immune response to the expressed antigen was reported in groups where vaccine and NTP were dispensed in conjunction as compared with animals that received the vaccine alone.…”

Section: Ntp For Vaccinationmentioning

confidence: 99%

Nonthermal plasma as part of a novel strategy for vaccination

Mohamed

Esposito

Kutzler

et al. 2020

Plasma Processes & Polymers

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Vaccination has been one of the most effective health intervention mechanisms to reduce morbidity and mortality associated with infectious diseases. Vaccines stimulate the body's protective immune responses through controlled exposure to modified versions of pathogens that establish immunological memory. However, only a few diseases have effective vaccines. The biological effects of nonthermal plasma on cells suggest that plasma could play an important role in improving efficacy of existing vaccines and overcoming some of the limitations and challenges with current vaccination strategies. This review summarizes the opportunities for nonthermal plasma for immunization and therapeutic purposes.

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“…The permeability of cells exposed to gas plasma, either in vivo or in vitro , is increased and delivery of macromolecular cargos such as plasmid DNA can be achieved. 66 For the past decades, several plasma generation methods have been developed, including atmospheric discharge plasma 67 , atmospheric helium plasma 68–70 , non-thermal plasma 71 , shielded sliding discharge plasma 72 , atmospheric-pressure plasma 73 , and plasma-activated air 74 . Using cold atmospheric plasma generated by a plasma jet system, Kong and co-coworkers recently established that ROS production is involved in plasma-induced cell permeabilization.…”

Section: Relevance Of Mammalian Membrane Oxidation In Intracellulamentioning

confidence: 99%

Membrane Oxidation in Cell Delivery and Cell Killing Applications

2017

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Cell delivery or cell killing processes often involve the crossing or disruption of cellular membranes. We review how, by modifying the composition and properties of membranes, membrane oxidation can be exploited to enhance the delivery of macromolecular cargos into live human cells. We also describe how membrane oxidation can be utilized to achieve efficient killing of bacteria by antimicrobial peptides. Finally, we present recent evidence highlighting how membrane oxidation is intimately engaged in natural biological processes such as antigen delivery in dendritic cells and in the killing of bacteria by human macrophages. Overall, the insights that have been recently gained in this area should facilitate the development of more effective delivery technologies and antimicrobial therapeutic approaches.

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Non-contact helium-based plasma for delivery of DNA vaccines

Cited by 18 publications

References 31 publications

Comparison between cold plasma, electrochemotherapy and combined therapy in a melanoma mouse model

Comparison between cold plasma, electrochemotherapy and combined therapy in a melanoma mouse model

Nonthermal plasma as part of a novel strategy for vaccination

Membrane Oxidation in Cell Delivery and Cell Killing Applications

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