Non-Coding RNAs in Stem Cell Regulation and Cardiac Regeneration: Current Problems and Future Perspectives

Schweiger, Victor; Hašimbegović, Ena; Kastner, Nina; Spannbauer, Andreas; Traxler, Denise; Gyöngyösi, Mariann; Mester-Tonczar, Julia

doi:10.3390/ijms22179160

Cited by 6 publications

(5 citation statements)

References 112 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Circamotl1 Activates Akt Promoting Cardiomyocytes Proliferat...mentioning

confidence: 99%

“…Myocardial injury leads to cardiomyocyte death as a consequence of oxygen and nutrient loss, impaired cardiac remodeling, and compromised regenerative abilities [104]. Several studies have described the altered expression of different circRNAs in the damaged myocardium, including circFndc3b, circ-Ttc3, and many others [105,106], which showed protective properties through accelerating the regeneration of cardiomyocytes [104,107]. CircAMOTL1 (hsa_circ_0004214) was found to be a circRNA with higher expression in neonatal hearts as compared to the mature myocardium, suggesting its potential function in cardiomyocyte survival and proliferation [87].…”

Section: Circamotl1 Activates Akt Promoting Cardiomyocytes Proliferat...mentioning

confidence: 99%

See 1 more Smart Citation

CircAMOTL1 RNA and AMOTL1 Protein: Complex Functions of AMOTL1 Gene Products

Sadlak

Joshi

Prószyński

et al. 2023

IJMS

View full text Add to dashboard Cite

The complexity of the cellular proteome facilitates the control of a wide range of cellular processes. Non-coding RNAs, including microRNAs and long non-coding RNAs, greatly contribute to the repertoire of tools used by cells to orchestrate various functions. Circular RNAs (circRNAs) constitute a specific class of non-coding RNAs that have recently emerged as a widely generated class of molecules produced from many eukaryotic genes that play essential roles in regulating cellular processes in health and disease. This review summarizes current knowledge about circRNAs and focuses on the functions of AMOTL1 circRNAs and AMOTL1 protein. Both products from the AMOTL1 gene have well-known functions in physiology, cancer, and other disorders. Using AMOTL1 as an example, we illustrate how focusing on both circRNAs and proteins produced from the same gene contributes to a better understanding of gene functions.

show abstract

Section: Circamotl1 Activates Akt Promoting Cardiomyocytes Proliferat...mentioning

confidence: 99%

Section: Circamotl1 Activates Akt Promoting Cardiomyocytes Proliferat...mentioning

confidence: 99%

CircAMOTL1 RNA and AMOTL1 Protein: Complex Functions of AMOTL1 Gene Products

Sadlak

Joshi

Prószyński

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…NcRNAs have been shown to regulate cellular reprogramming, pluripotency, cardiac proliferation, differentiation and maturation. Modulation of ncRNAs may enhance the quality and quantity of stem cells and their derivatives for potential clinical application in cardiac patients [120,121]. However, the role of ncRNA-mediated regulation of stem cells in BrS is unclear and deserves future attention.…”

Section: Noncoding Rnas and Stem Cells In Brsmentioning

confidence: 99%

“…quality and quantity of stem cells and their derivatives for potential clinical applicati cardiac patients [120,121]. However, the role of ncRNA-mediated regulation of stem in BrS is unclear and deserves future attention.…”

Section: Noncoding Rnas and Stem Cells In Brsmentioning

confidence: 99%

Noncoding RNAs and Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Cardiac Arrhythmic Brugada Syndrome

Theisen,

Holtz,

Rajagopalan

2023

Cells

View full text Add to dashboard Cite

Hundreds of thousands of people die each year as a result of sudden cardiac death, and many are due to heart rhythm disorders. One of the major causes of these arrhythmic events is Brugada syndrome, a cardiac channelopathy that results in abnormal cardiac conduction, severe life-threatening arrhythmias, and, on many occasions, death. This disorder has been associated with mutations and dysfunction of about two dozen genes; however, the majority of the patients do not have a definite cause for the diagnosis of Brugada Syndrome. The protein-coding genes represent only a very small fraction of the mammalian genome, and the majority of the noncoding regions of the genome are actively transcribed. Studies have shown that most of the loci associated with electrophysiological traits are located in noncoding regulatory regions and are expected to affect gene expression dosage and cardiac ion channel function. Noncoding RNAs serve an expanding number of regulatory and other functional roles within the cells, including but not limited to transcriptional, post-transcriptional, and epigenetic regulation. The major noncoding RNAs found in Brugada Syndrome include microRNAs; however, others such as long noncoding RNAs are also identified. They contribute to pathogenesis by interacting with ion channels and/or are detectable as clinical biomarkers. Stem cells have received significant attention in the recent past, and can be differentiated into many different cell types including those in the heart. In addition to contractile and relaxational properties, BrS-relevant electrophysiological phenotypes are also demonstrated in cardiomyocytes differentiated from stem cells induced from adult human cells. In this review, we discuss the current understanding of noncoding regions of the genome and their RNA biology in Brugada Syndrome. We also delve into the role of stem cells, especially human induced pluripotent stem cell-derived cardiac differentiated cells, in the investigation of Brugada syndrome in preclinical and clinical studies.

show abstract

“…More and more evidences have supported that non-coding RNAs (ncRNAs) play important roles of post-transcriptional regulation in cardiomyocyte (CM) differentiation [ 7 ]. The long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) could serve as microRNA (miRNA) sponges and then indirectly regulate the expression levels of developmental genes via the “lnc/circRNA-miRNA-mRNA” regulatory circuits, which consist of the competing endogenous RNA (ceRNA) network [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Competing endogenous RNA network analysis of Turner syndrome patient-specific iPSC-derived cardiomyocytes reveals dysregulation of autosomal heart development genes by altered dosages of X-inactivation escaping non-coding RNAs

Luo,

Chen,

et al. 2023

Stem Cell Res Ther

View full text Add to dashboard Cite

Background A 45,X monosomy (Turner syndrome, TS) is the only chromosome haploinsufficiency compatible with life. Nevertheless, the surviving TS patients still suffer from increased morbidity and mortality, with around one-third of them subjecting to heart abnormalities. How loss of one X chromosome drive these conditions remains largely unknown. Methods Here, we have generated cardiomyocytes (CMs) from wild-type and TS patient-specific induced pluripotent stem cells and profiled the mRNA, lncRNA and circRNA expression in these cells. Results We observed lower beating frequencies and higher mitochondrial DNA copies per nucleus in TS-CMs. Moreover, we have identified a global transcriptome dysregulation of both coding and non-coding RNAs in TS-CMs. The differentially expressed mRNAs were enriched of heart development genes. Further competing endogenous RNA network analysis revealed putative regulatory circuit of autosomal genes relevant with mitochondrial respiratory chain and heart development, such as COQ10A, RARB and WNT2, mediated by X-inactivation escaping lnc/circRNAs, such as lnc-KDM5C-4:1, hsa_circ_0090421 and hsa_circ_0090392. The aberrant expressions of these genes in TS-CMs were verified by qPCR. Further knockdown of lnc-KDM5C-4:1 in wild-type CMs exhibited significantly reduced beating frequencies. Conclusions Our study has revealed a genomewide ripple effect of X chromosome halpoinsufficiency at post-transcriptional level and provided insights into the molecular mechanisms underlying heart abnormalities in TS patients.

show abstract

Non-Coding RNAs in Stem Cell Regulation and Cardiac Regeneration: Current Problems and Future Perspectives

Cited by 6 publications

References 112 publications

CircAMOTL1 RNA and AMOTL1 Protein: Complex Functions of AMOTL1 Gene Products

CircAMOTL1 RNA and AMOTL1 Protein: Complex Functions of AMOTL1 Gene Products

Noncoding RNAs and Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Cardiac Arrhythmic Brugada Syndrome

Competing endogenous RNA network analysis of Turner syndrome patient-specific iPSC-derived cardiomyocytes reveals dysregulation of autosomal heart development genes by altered dosages of X-inactivation escaping non-coding RNAs

Contact Info

Product

Resources

About