Non-coding RNAs in polycystic ovary syndrome: a systematic review and meta-analysis

Mu, Liangshan; Sun, Xiaoting; Tu, Mixue; Zhang, Dan

doi:10.1186/s12958-020-00687-9

Cited by 54 publications

(33 citation statements)

References 115 publications

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“…Many studies have found that a variety of miRNA and long-chain non-coding RNA (lncRNA) can regulate the progress of PCOS. However, few studies have investigated the role of circRNAs in PCOS (23). So far, four studies have investigated the difference in circRNA between PCOS patients and controls, in which hsa_circ_0083952, hsa_ circ_0082709, hsa_circ_0002425, and hsa_circ_0015168, etc.…”

Section: Discussionmentioning

confidence: 99%

“…These results indicate that circRHBG acts as a miR-515-5p sponge in PCOS. miRNA usually inhibits the expression of the target gene by binding to the 3'-UTR of the targeted mRNA to degrade it (23). To explore the target molecules of miR-515-5p, we used the TargetScan algorithm to identify SLC7A11 as a candidate target oncogene (Figure 4A).…”

Section: Circrhbg Functions As a Sponge For Mir-515-5pmentioning

confidence: 99%

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Involvement of ferroptosis in the granulosa cells proliferation of PCOS through the circRHBG/miR-515/SLC7A11 axis

Zhang¹,

Yi²,

Cai³

et al. 2021

Ann Transl Med

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Background: Polycystic ovary syndrome (PCOS) is the most common endocrine disease among women, but its etiology remains unknown. In recent years, many circular RNAs (circRNAs) have been confirmed to be related to the development of PCOS. The role and mechanism of circRNA in the development of PCOS need to be further explored.Methods: In the present study, we used the circRNA chip to detect the difference in the expression of circRNA in the granulosa cells of PCOS patients and controls. Five upregulated circRNAs were then selected for quantitative reverse transcription polymerase chain reaction (qRT-PCR) verification, and circRHBG was found to be upregulated in PCOS. Subsequently, Cell Counting Kit-8 assay and EdU assay were used to observe the effect of circRHBG on the proliferation of KGN and SVOG cells. Furthermore, the pairwise binding relationship between circRHBG/miR515-5 and miR515-5p/SLC7A11 was verified by luciferase reporter assay. The interaction between circRHBG and SLC7A11 was detected with qRT-PCR and Western blot.Results: CircRNA high-throughput chips and qRT-PCR verified that circRHBG was significantly upregulated in granular cells of PCOS patients. Knockdown of circRHBG inhibits KGN and SVOG cell proliferation. Luciferase reporter assays and Ago2-RIP detection showed that circRHBG competes with SLC7A11 to bind to miR-515-5p. Subsequent experiments verified knockdown of circRHBG promotes ferroptosis in PCOS.Conclusions: circRHBG inhibits ferroptosis in PCOS cells through the circRHBG/miR-515-5p/ SLC7A11 axis in PCOS, which may provide new diagnostic molecular markers and therapeutic targets for PCOS.

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Section: Discussionmentioning

confidence: 99%

Section: Circrhbg Functions As a Sponge For Mir-515-5pmentioning

confidence: 99%

Involvement of ferroptosis in the granulosa cells proliferation of PCOS through the circRHBG/miR-515/SLC7A11 axis

Zhang¹,

Yi²,

Cai³

et al. 2021

Ann Transl Med

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“…Knowledge on the role of miRNAs in the regulation of metabolism and development of metabolic diseases is developing intensively [276]. The role of miRNAs in the development of metabolic diseases such as obesity, T2D, polycystic ovary syndrome, nonalcoholic fatty disease, cardiovascular and inflammatory diseases was recently reviewed [277][278][279][280][281][282][283]. Moreover, the expression of aquaporin isoforms associated with metabolic diseases is also regulated by microRNAs [284].…”

Section: Micrornas Expressed In Adipose Tissue Are Involved In Molecular Mechanism Of Metabolic Diseasesmentioning

confidence: 99%

“…Moreover, the expression of aquaporin isoforms associated with metabolic diseases is also regulated by microRNAs [284]. Studies describing the regulation of microRNAs expression by gonadal steroids [34][35][36][37] and the association of microRNAs with metabolic diseases [277][278][279][280][281][283][284][285], allowed to identify the most important microRNAs in metabolic diseases which expression level may be regulated by sex hormones (Table 2).…”

Section: Micrornas Expressed In Adipose Tissue Are Involved In Molecular Mechanism Of Metabolic Diseasesmentioning

confidence: 99%

Recent Update on the Molecular Mechanisms of Gonadal Steroids Action in Adipose Tissue

Wawrzkiewicz-Jałowiecka

Lalik

Soveral

2021

IJMS

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The gonadal steroids, including androgens, estrogens and progestogens, are involved in the control of body fat distribution in humans. Nevertheless, not only the size and localization of the fat depots depend on the sex steroids levels, but they can also highly affect the functioning of adipose tissue. Namely, the gonadocorticoids can directly influence insulin signaling, lipid metabolism, fatty acid uptake and adipokine production. They may also alter energy balance and glucose homeostasis in adipocytes in an indirect way, e.g., by changing the expression level of aquaglyceroporins. This work presents the recent advances in understanding the molecular mechanism of how the gonadal steroids influence the functioning of adipose tissue leading to a set of detrimental metabolic consequences. Special attention is given here to highlighting the sexual dimorphism of adipocyte functioning in terms of health and disease. Particularly, we discuss the molecular background of metabolic disturbances occurring in consequence of hormonal imbalance which is characteristic of some common endocrinopathies such as the polycystic ovary syndrome. From this perspective, we highlight the potential drug targets and the active substances which can be used in personalized sex-specific management of metabolic diseases, in accord with the patient’s hormonal status.

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“…Multiple genome-wide studies suggested that epigenetic factors, such as non-coding RNAs and DNA methylation, are closely associated with PCOS ( Yu Y.Y. et al, 2015 ; Mu et al, 2021 ). microRNA is involved in proliferation, apoptosis, and steroid production of ovarian cells ( Sirotkin et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of DNA Methylation and Screening of Epigenetic Markers in Polycystic Ovary Syndrome

Cao

Yang

Wang

et al. 2021

Front. Cell Dev. Biol.

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Polycystic ovary syndrome (PCOS) is a heterogeneous endocrine and metabolic disorder in women, which is characterized by androgen excess, ovulation dysfunction, and polycystic ovary. Although the etiology of PCOS is largely unknown, many studies suggest that aberrant DNA methylation is an important contributing factor for its pathological changes. In this study, we investigated DNA methylation characteristics and their impact on gene expression in granulosa cells obtained from PCOS patients. Transcriptome analysis found that differentially expressed genes were mainly enriched in pathways of insulin resistance, fat cell differentiation, and steroid metabolism in PCOS. Overall DNA methylation level in granulosa cells was reduced in PCOS, and the first introns were found to be the major genomic regions that were hypomethylated in PCOS. Integrated analysis of transcriptome, DNA methylation, and miRNAs in ovarian granulosa cells revealed a DNA methylation and miRNA coregulated network and identified key candidate genes for pathogenesis of PCOS, including BMP4, ETS1, and IRS1. Our study shed more light on epigenetic mechanism of PCOS and provided valuable reference for its diagnosis and treatment.

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Non-coding RNAs in polycystic ovary syndrome: a systematic review and meta-analysis

Cited by 54 publications

References 115 publications

Involvement of ferroptosis in the granulosa cells proliferation of PCOS through the circRHBG/miR-515/SLC7A11 axis

Involvement of ferroptosis in the granulosa cells proliferation of PCOS through the circRHBG/miR-515/SLC7A11 axis

Recent Update on the Molecular Mechanisms of Gonadal Steroids Action in Adipose Tissue

Characterization of DNA Methylation and Screening of Epigenetic Markers in Polycystic Ovary Syndrome

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