Non-coding RNAs in Host–Pathogen Interactions: Subversion of Mammalian Cell Functions by Protozoan Parasites

Bayer-Santos, Ethel; Marini, Marjorie Mendes; Silveira, José Franco da

doi:10.3389/fmicb.2017.00474

Cited by 40 publications

(29 citation statements)

References 71 publications

(87 reference statements)

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“…Very recently, Dandewad and coworkers described the retrograde mechanism that involves a transport of human miRNA-RISC complexes into Plasmodium falciparum cells and a further regulatory mechanism of the parasite mRNA targets [139]. These combined mechanisms demonstrate an intimate molecular cross-communication between the malaria parasite and its host where ncRNAs are important players and could be extensive to other protozoa [140,141] and helmintic parasites [142].…”

Section: Mammalsmentioning

confidence: 99%

Interspecies Communication in Holobionts by Non-Coding RNA Exchange

Leitão

Costa

Gabriel

et al. 2020

IJMS

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Complex organisms are associations of different cells that coexist and collaborate creating a living consortium, the holobiont. The relationships between the holobiont members are essential for proper homeostasis of the organisms, and they are founded on the establishment of complex inter-connections between all the cells. Non-coding RNAs are regulatory molecules that can also act as communication signals between cells, being involved in either homeostasis or dysbiosis of the holobionts. Eukaryotic and prokaryotic cells can transmit signals via non-coding RNAs while using specific extracellular conveyors that travel to the target cell and can be translated into a regulatory response by dedicated molecular machinery. Within holobionts, non-coding RNA regulatory signaling is involved in symbiotic and pathogenic relationships among the cells. This review analyzes current knowledge regarding the role of non-coding RNAs in cell-to-cell communication, with a special focus on the signaling between cells in multi-organism consortia.

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Section: Mammalsmentioning

confidence: 99%

Interspecies Communication in Holobionts by Non-Coding RNA Exchange

Leitão

Costa

Gabriel

et al. 2020

IJMS

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“…Recently, two novel "star non-coding RNAs (ncR-NAs)", namely long non-coding RNA (lncRNA) and circular RNA (circRNA), have been reported to play pivotal role in the interaction between hosts and bacteria [25,26], viruses [27][28][29][30] and parasites [31][32][33]. For example, using the RNA-seq technique, the expression profiles of mRNAs, lncRNAs and circRNAs of tracheal tissues during Cryptosporidium baileyi infection were studied, and functional analysis of these differentially expressed RNAs were related to the immune system process, immune response and leukocyte activation, tight junction and glycerolipid metabolism, suggesting the important role of these RNAs in interaction between chickens and C. baileyi [33].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of differentially expressed profiles of mRNAs, lncRNAs and circRNAs in chickens during Eimeria necatrix infection

et al. 2020

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Background: Eimeria necatrix, the most highly pathogenic coccidian in chicken small intestines, can cause high morbidity and mortality in susceptible birds and devastating economic losses in poultry production, but the underlying molecular mechanisms in interaction between chicken and E. necatrix are not entirely revealed. Accumulating evidence shows that the long-non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) are key regulators in various infectious diseases. However, the expression profiles and roles of these two non-coding RNAs (ncRNAs) during E. necatrix infection are still unclear. Methods: The expression profiles of mRNAs, lncRNAs and circRNAs in mid-segments of chicken small intestines at 108 h post-infection (pi) with E. necatrix were analyzed by using the RNA-seq technique. Results: After strict filtering of raw data, we putatively identified 49,183 mRNAs, 818 lncRNAs and 4153 circRNAs. The obtained lncRNAs were classified into four types, including 228 (27.87%) intergenic, 67 (8.19%) intronic, 166 (20.29%) anti-sense and 357 (43.64%) sense-overlapping lncRNAs; of these, 571 were found to be novel. Five types were also predicted for putative circRNAs, including 180 exonic, 54 intronic, 113 antisense, 109 intergenic and 3697 senseoverlapping circRNAs. Eimeria necatrix infection significantly altered the expression of 1543 mRNAs (707 upregulated and 836 downregulated), 95 lncRNAs (49 upregulated and 46 downregulated) and 13 circRNAs (9 upregulated and 4 downregulated). Target predictions revealed that 38 aberrantly expressed lncRNAs would cis-regulate 73 mRNAs, and 1453 mRNAs could be trans-regulated by 87 differentially regulated lncRNAs. Additionally, 109 potential sponging miRNAs were also identified for 9 circRNAs. GO and KEGG enrichment analysis of target mRNAs for lncRNAs, and sponging miRNA targets and source genes for circRNAs identified associations of both lncRNAs and circRNAs with host immune defense and pathogenesis during E. necatrix infection. Conclusions: To the best of our knowledge, the present study provides the first genome-wide analysis of mRNAs, lncRNAs and circRNAs in chicken small intestines infected with E. necatrix. The obtained data will offer novel clues for exploring the interaction mechanisms between chickens and Eimeria spp.

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“…Down-regulation of several ncRNAs upon infection with MVs also raise interesting questions on the role of this class of RNAs in L. monocytogenes infection. Several reports on ncRNAs suggested that the regulatory RNAs used by intracellular pathogens may aid to survive and evade immune responses (56,57).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome responses of intestinal epithelial cells induced by membrane vesicles ofListeria monocytogenesunveil novel insights into the host-pathogen cross talk

Karthikeyan

Gayathri

Gunasekaran

et al. 2019

Preprint

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32Membrane vesicles (MVs) serve as a vital source of virulence factors in many pathogenic organisms. The 33 release of MVs by Listeria monocytogenes is only recently recognized, but its role in the pathogenesis is 34 poorly understood. Here, we investigated the role of MVs of L. monocytogenes in virulence and host 35interactions. Proteomic analyses of whole cells and MVs of L. monocytogenes were performed using 36 LC/MS/MS. A total of 1376 and 456 proteins were identified in the L. monocytogenes cells and MVs, 37 respectively. Also, we have found that MVs contains active pore-forming listeriolysin (LLO), internalin 38 B (inlB), phosphatidylinositol-specific phospholipase C (PI-PLC-A). We have previously reported that 39MVs of L. monocytogenes can infect and induce cytotoxicity in Caco-2 cells. In this study, we report the 40 transcriptome response of Caco-2 cells upon infection with MVs as well as L. monocytogenes. In 41 particular, we observed the up-regulation of autophagy-related genes in the early phase of infection with 42 MVs. Transcription of inflammatory cytokines (CCL2, CXCL6, CXCL8, CXCL15, CXCL5, CXCL10) 43 peaked at four h of infection. A large number of differentially expressed genes was associated with actin 44 cytoskeleton rearrangement, autophagy, cell cycle arrest, and induction of oxidative stress. At a later time 45 point, transcriptional programs generated upon infection with MVs point toward to evade innate immune 46 signals, by modulating the expression of anti-inflammatory genes. KEGG pathway enrichment analysis 47 revealed that MVs induce several signaling pathways such as PI3k-Akt signaling pathway, mitogen-48 activated protein kinase (MAPK) pathway, NOD-like receptor signaling pathway, cAMP signaling 49 pathway, TNF, and NF-kB signaling pathway. Moreover, MVs induced the expression of cell cycle 50 regulatory genes, which may result in the ability to prolong host cell survival, thus protecting the 51 replicative niche for L. monocytogenes. Notably, we identified several non-coding RNAs (ncRNAs) are 52 regulated during infection, suggesting that an early manipulation of the host gene expression may be 53 essential for L. monocytogenes persistence and replication in host cells. 54 55 56 57 58 59 60 61 62 63 64 Introduction 65Several Gram-negative and Gram-positive bacteria release membrane vesicles (MVs). MVs are a 66 spherical structure of 50-300 nm in diameter composed of the single membrane that is derived from the 67 outer membrane (OM) and contains OM proteins, lipopolysaccharide (LPS), and other lipids, while the 68 vesicle lumen mainly contains periplasmic proteins (1). Many pathogenic Gram-negative bacteria species 69 extend their virulence potential by releasing spherical buds, derived from the outer membrane, so-called 70 outer membrane vesicles (2). The release of MVs can benefit the microbe, mediating microbial 71 interactions with the human host and within bacterial communities (3,2). Also, MV production protects 72 bacteria under stressful conditions by eliminating the accumu...

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Non-coding RNAs in Host–Pathogen Interactions: Subversion of Mammalian Cell Functions by Protozoan Parasites

Cited by 40 publications

References 71 publications

Interspecies Communication in Holobionts by Non-Coding RNA Exchange

Interspecies Communication in Holobionts by Non-Coding RNA Exchange

Genome-wide analysis of differentially expressed profiles of mRNAs, lncRNAs and circRNAs in chickens during Eimeria necatrix infection

Transcriptome responses of intestinal epithelial cells induced by membrane vesicles ofListeria monocytogenesunveil novel insights into the host-pathogen cross talk

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