Non-Coding RNAs in Cardiac Aging

Wang, Hui; Bei, Yihua; Shi, Jing; Xiao, Junjie; Kong, Xiangqing

doi:10.1159/000430141

Cited by 19 publications

(17 citation statements)

References 57 publications

(67 reference statements)

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“…These biological processes regulated by lncRNAs include invasion, metastasis, apoptosis, DNA damage, angiogenesis, microRNA (miR/miRNA) silencing, embryonic development, cardiac aging and tumor development [8-11]. Recently, few literatures have focused on the role of lncRNAs in AMI.…”

Section: Introductionmentioning

confidence: 99%

Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139

Gong¹,

Xu²,

Guo³

et al. 2017

Cell Physiol Biochem

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Background/Aims: Acute myocardial infarction (AMI) occurs when blood supply to the heart is diminished (ischemia) for long time; ischemia is primarily caused due to hypoxia. The present study evaluated the effects of long non-coding RNA H19 on hypoxic rat H9c2 cells and mouse HL-1 cells. Methods: Hypoxic injury was confirmed by measuring cell viability, migration and invasion, and apoptosis using MTT, Transwell and flow cytometry assays, respectively. H19 expression after hypoxia was estimated by qRT-PCR. We then measured the effects of non-physiologically expressed H19, knockdown of miR-139 with or without H19 silence, and abnormally expressed Sox8 on hypoxia-induced H9c2 cells. Moreover, the interacted miRNA for H19 and downstream target gene were virtually screened and verified. The involved signaling pathways and the effects of abnormally expressed H19 on contractility of HL-1 cells were explored via Western blot analysis. Results: Hypoxia induced decreases of cell viability, migration and invasion, increase of cell apoptosis and up-regulation of H19. Knockdown of H19 increased hypoxia-induced injury in H9c2 cells. H19 acted as a sponge for miR-139 and H19 knockdown aggravated hypoxia-induced injury by up-regulating miR-139. Sox8 was identified as a target of miR-139, and its expression was negatively regulated by miR-139. The mechanistic studies revealed that overexpression of Sox8 might decrease hypoxia-induced cell injury by activating the PI3K/AKT/mTOR pathway and MAPK. Besides, H19 promoted contractility of HL-1 cells. Conclusion: These findings suggest that H19 alleviates hypoxia-induced myocardial cell injury by miR-139-mediated up-regulation of Sox8, along with activation of the PI3K/AKT/mTOR pathway and MAPK.

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Section: Introductionmentioning

confidence: 99%

Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139

Gong¹,

Xu²,

Guo³

et al. 2017

Cell Physiol Biochem

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“…The identification of some overlaps in their biogenesis pathways and functional roles raises the hypothesis that these molecules play concerted functions in vivo, creating complex regulatory networks where cooperation with regulatory proteins is necessary. The implications of biogenesis and gene expression deregulation of sncRNAs and lncRNAs were also highlighted in a number of human diseases like cardiac ageing [52], gastric, and other types of cancer [53,54]. Over the recent years, Next Generation Sequencing (NGS) technologies targeting the micro RNA transcriptome revealed the existence of many different RNA fragments derived from small RNA species other than micro RNA.…”

Section: Resultsmentioning

confidence: 99%

Peculiarities of Non Coding Micrornas Organization in H. Sapiens and some other Species

Ns¹

2017

IJMBOA

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It is universally accepted today that micro (mi) non coding (nc) RNAs are endogenous ~22 nt RNAs that can play important regulatory roles in a vast number of cellular processes including cell growth, disease, embryogenesis, gene regulation, signal transduction, receptor activation, etc. by targeting mRNAs for cleavage or translational repression. Mi RNAs comprise one of the more abundant classes of gene regulatory molecules in multi cellular organisms and likely influence the output of many protein-coding genes. The shorter lin-4 RNA is now recognized as the founding member of an abundant class of tiny regulatory RNAs called micro RNAs or mi RNAs. Now it has become clear that large number of regulatory small (rs) RNAs are compromised due to traditional approaches to identify mi RNAs mainly to hair-pin loop bred typical signs. The rsRNA-target interactions have been studied with the use of mi RNA recognition elements binding with Ago proteins (AGO-cross linking), microprocessor complex subunit DGCR8 involved in the initial step of mi RNA biogenesis cross-linking, ligation, and sequencing of hybrids (CLASH), proteome and expression data. The advent of next generation sequencing (NGS) technologies, powerful enough to detect most of the existing small (s) RNA sequences even with low abundance, gave rise to a sudden surge in the reporting of novel mi RNAs.

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“…lncRNAs are key regulator of the aging process and play and important role in the onset and progression of cardiac aging (174,175). Senescence-associated lncRNAs (SAL-RNAs) may indeed influence the aging phenotype, regulating cardiac function and myocardial fibrosis (176).…”

Section: Micrornas (Mirnas) and Long Non Coding Rnas (Lncrna)mentioning

confidence: 99%

“…Senescence-associated lncRNAs (SAL-RNAs) may indeed influence the aging phenotype, regulating cardiac function and myocardial fibrosis (176). In particular, SAL-RNA1 appears to delay cardiac aging (176) while SAL-RNA2 and SAL-RNA3 promote the survival of the senescent fibroblasts through the increased expression of p53 (174). Furthermore, the lncRNA H19 is an important modulator of the aging process, negatively affecting cell proliferation and promoting cellular senescence (177,178).…”

Section: Micrornas (Mirnas) and Long Non Coding Rnas (Lncrna)mentioning

confidence: 99%

Role of circulating factors in cardiac aging

Cannatà¹,

Marcon²,

Cimmino³

et al. 2017

J. Thorac. Dis.

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Worldwide increase in life expectancy is a major contributor to the epidemic of chronic degenerative diseases. Aging, indeed, simultaneously affects multiple organ systems, and it has been hypothesized that systemic alterations in regulators of tissue physiology may regulate this process. Cardiac aging itself is a major risk factor for cardiovascular diseases and, because of the intimate relationship with the brain, may contribute to increase the risk of neurodegenerative disorders. Blood-borne factors may play a major role in this complex and still elusive process. A number of studies, mainly based on the revival of parabiosis, a surgical technique very popular during the 70s of the 20 th century to study the effect of a shared circulation in two animals, have indeed shown the potential that humoral factors can control the aging process in different tissues. In this article we review the role of circulating factors in cardiovascular aging. A better understanding of these mechanisms may provide new insights in the aging process and provide novel therapeutic opportunities for chronic age-related disorders.

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Non-Coding RNAs in Cardiac Aging

Cited by 19 publications

References 57 publications

Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139

Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139

Peculiarities of Non Coding Micrornas Organization in H. Sapiens and some other Species

Role of circulating factors in cardiac aging

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