Non-Coding RNA Signatures of B-Cell Acute Lymphoblastic Leukemia

Rodriguez, Princess D.; Paculova, Hana; Kogut, Sophie; Heath, Jessica L.; Schjerven, Hilde; Frietze, Seth

doi:10.20944/preprints202102.0496.v1

Cited by 10 publications

(5 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A hiPS cell line harboring the leukemia‐specific fusion gene ETV6‐RUNX1 [Takahashi and Yamazaki, 2019 ] would be useful for focusing on the participation of MFs in the development of leukemia from a pre‐leukemic state. Moreover, determining whether MFs contribute to some mechanisms of leukemogenesis without genetic changes, namely epigenetic alterations such as DNA methylation [Dunwell et al, 2009 ], histone modification [Li et al, 2010 ; Swaroop et al, 2019 ], and alterations of noncoding RNAs [Rodriguez et al, 2021 ], is also crucial.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Effects of Exposure to Power‐Frequency Magnetic Fields on the Differentiation of Hematopoietic Stem/Progenitor Cells Using Human‐Induced Pluripotent Stem Cells

Takahashi

Furuya²

2022

Bioelectromagnetics

View full text Add to dashboard Cite

The causal association between exposure to power‐frequency magnetic fields (MFs) and childhood leukemia has been under discussion. Although evidence from experimental studies is required for a conclusion to be reached, only a few studies have focused on the effects of MF exposure on the human hematopoietic system directly related to leukemogenesis. Here, we established an in vitro protocol to simulate the differentiation of human mesodermal cells to hematopoietic stem progenitor cells (HSPCs) using human‐induced pluripotent stem cells. Furthermore, we introduced MF in the protocol to study the effects of exposure. After a continuous exposure to 0–300 mT of 50‐Hz MFs during the differentiation process, the efficiency of differentiation of mesodermal cells into HSPCs was analyzed in a single‐blinded manner. The percentage of emerged HSPCs from mesodermal cells in groups exposed to 50‐Hz MFs indicated a lack of significant changes compared with those in the sham‐exposed group. These results suggest that exposure to 50‐Hz MFs up to 300 mT does not affect the differentiation of human mesodermal cells to HSPCs, which may be involved in the initial process of leukemogenesis. © 2022 The Authors. Bioelectromagnetics published by Wiley Periodicals LLC on behalf of Bioelectromagnetics Society.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of the Effects of Exposure to Power‐Frequency Magnetic Fields on the Differentiation of Hematopoietic Stem/Progenitor Cells Using Human‐Induced Pluripotent Stem Cells

Takahashi

Furuya²

2022

Bioelectromagnetics

View full text Add to dashboard Cite

show abstract

“…123 BARL2 overexpression is also associated with poor therapeutic response and OS in B-ALL patients. 85…”

Section: Lncrnas In Leukemiamentioning

confidence: 99%

“…Moreover, Rao et al noted that the expression patterns of BARL2 (B‐ALL–associated long RNAs‐2) lncRNA could predict three common cytogenetic profiles of B‐ALL (ETV6‐RUNX1, TCF3‐PBX1, and MLL‐AF4) 123 . BARL2 overexpression is also associated with poor therapeutic response and OS in B‐ALL patients 85 …”

Section: Lncrnas In Human Diseasesmentioning

confidence: 99%

An overview of long noncoding RNAs: Biology, functions, therapeutics, analysis methods, and bioinformatics tools

Jafari-Raddani

Davoodi-Moghaddam

Yousefi

et al. 2022

Cell Biochemistry & Function

View full text Add to dashboard Cite

Long noncoding RNAs (lncRNAs) are a diverse class of RNAs whose functions are widespread in all branches of life and have been the focus of attention in the last decade. While a huge number of lncRNAs have been identified, there is still much work to be done and plenty to be learned. In the current review, we begin with the biogenesis and function of lncRNAs as they are involved in the different cellular processes from regulating the architecture of chromosomes to controlling translation and post‐translation modifications. Questions on how overexpression, mutations, or deficiency of lncRNAs can affect the cellular status and result in the pathogenesis of various human diseases are responded to. Besides, we allocate an overview of several studies, concerning the application of lncRNAs either as diagnostic and prognostic biomarkers or novel therapeutics. We also introduce the currently available techniques to explore details of lncRNAs such as their function, cellular localization, and structure. In the last section, as exponentially growing data in this area need to be gathered and organized in comprehensive databases, we have a particular focus on presenting general and specialized databases. Taken together, with this review, we aim to provide the latest information on different aspects of lncRNAs to highlight their importance in physiopathologic states and take a step towards helping future studies.

show abstract

“…Commonly, oncogenic miRNAs (oncomiRs) are overexpressed in cancers while tumor-suppressive miRNAs (tsmiRs) are lowly expressed. miRNAs have additionally been identified as candidates for diagnostic and prognostic biomarkers, as well as predictors of drug responses in cancer [25,26]. Importantly, miRNAs represent candidate clinical biomarkers that may act as indicators of relapse for B-ALL [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

“…miRNAs have additionally been identified as candidates for diagnostic and prognostic biomarkers, as well as predictors of drug responses in cancer [25,26]. Importantly, miRNAs represent candidate clinical biomarkers that may act as indicators of relapse for B-ALL [26][27][28]. However, there have been limited number of such studies and new clinically relevant biomarkers are still needed.…”

Section: Introductionmentioning

confidence: 99%

Ikaros Regulates microRNA Networks in Acute Lymphoblastic Leukemia

Kogut¹,

Paculova²,

Rodriguez³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

The hematopoietic transcription factor Ikaros (IKZF1) regulates normal B cell development and functions as a tumor suppressor in precursor B cell acute lymphoblastic leukemia (B-ALL). MicroRNAs (miRNAs) are small regulatory RNAs that through post-transcriptional gene regulation play critical roles in intracellular processes including cell growth in cancer. However, the role of Ikaros in the regulation of miRNA expression in developing B cells is unknown. In this study, we examined the Ikaros-regulated miRNA targets using patient-derived IKZF1-mutated B-ALL xenograft-derived cell lines. Inducible expression of wild-type Ikaros (the Ik1 isoform) caused B-ALL growth arrest and exit from the cell cycle. Global miRNA expression analysis revealed a total of 31 miRNAs regulated by IK1, and ChIP-seq analysis showed that Ikaros bound to several Ik1-responsive miRNA genes. Examination of the prognostic significance of miRNA expression in B-ALL indicate that the IK1-regulated miRNAs hsa-miR-26b, hsa-miR-130b and hsa-miR-4649 are significantly associated with outcome in B-ALL. Our findings establish a potential regulatory circuit between the tumor-suppressor Ikaros and the oncogenic miRNA networks in IKZF1-mutated B-ALL. These results indicate that Ikaros regulates the expression of a subset of miRNAs, of which several may contribute to B-ALL growth.

show abstract

Non-Coding RNA Signatures of B-Cell Acute Lymphoblastic Leukemia

Cited by 10 publications

References 89 publications

Evaluation of the Effects of Exposure to Power‐Frequency Magnetic Fields on the Differentiation of Hematopoietic Stem/Progenitor Cells Using Human‐Induced Pluripotent Stem Cells

Evaluation of the Effects of Exposure to Power‐Frequency Magnetic Fields on the Differentiation of Hematopoietic Stem/Progenitor Cells Using Human‐Induced Pluripotent Stem Cells

An overview of long noncoding RNAs: Biology, functions, therapeutics, analysis methods, and bioinformatics tools

Ikaros Regulates microRNA Networks in Acute Lymphoblastic Leukemia

Contact Info

Product

Resources

About