Non-canonical targets play an important role in microRNA stability control mechanisms

Park, June Hyun; Shin, Chanseok

doi:10.5483/bmbrep.2017.50.4.029

Cited by 4 publications

(2 citation statements)

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“…These results indicated that LINC00968 could bind to hsa-miR -423-5p. It is known that miRNAs play a role in RNA silencing by forming an RNA-induced silencing complex (RISC), such as Ago protein family that binds to and stabilize mature miRNAs [23]. Therefore, we determined whether LINC00968 and hsa-miR-423-5p formed RISC together.…”

Section: Linc00968 Binds To Hsa-mir-423-5pmentioning

confidence: 99%

RETRACTED ARTICLE: Long non-coding RNA LINC00968 reduces cell proliferation and migration and angiogenesis in breast cancer through up-regulation of PROX1 by reducing hsa-miR-423-5p

et al. 2019

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Background: Breast cancer (BC) is a common invasive malignancy in women with unclear etiology. A recent study suggested that long non-coding RNA (lncRNA), LINC00968 had a tumor-promoting effect in cancer. However, the role of LINC00968 in BC remains unclear. Therefore, we conducted the present study to determine the effect of LINC00968 in BC and its underlying mechanism. Methods: The expression of LINC00968 and hsa-miR-423-5p in BC tissues and cells was determined using reverse transcription quantitative polymerase chain reaction and western blot analysis. Dual luciferase reporter, RNA pull-down and RNA immunoprecipitation assays were used to determine the relationship among LINC00968, PROX1 and hsa-miR-423-5p. Gain-and loss-function approaches were utilized to examine the effects of LINC00968, PROX1 and hsa-miR-423-5p on cell proliferation, migration, tube formation in vitro; and tumor growth and angiogenesis in vivo. Results: LINC00968 expression reduced while hsa-miR-423-5p increased in BC tissues relative to adjacent normal tissues. Overexpression of LINC00968 was observed to inhibit BC cell proliferation, migration and tube formation abilities in vitro as well as tumor growth in vivo through inhibition of hsa-miR-423-5p. And hsa-miR-423-5p mediated BC cellular functions and tumor growth through down-regulating PROX1. LINC00968 was identified as a competing endogenous RNA to upregulate PROX1 by downregulating hsa-miR-423-5p. More importantly, it was found that LINC00968 increased PROX1 expression in vivo in a concentration-dependent manner. Conclusion: Taken together, this study suggests that LINC00968 inhibits the progression of BC through impeding hsa-miR-423-5p-mediated PROX1 inhibition. LINC00968 may be a potential therapeutic target for BC therapy that warrants further studies.

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Section: Linc00968 Binds To Hsa-mir-423-5pmentioning

confidence: 99%

RETRACTED ARTICLE: Long non-coding RNA LINC00968 reduces cell proliferation and migration and angiogenesis in breast cancer through up-regulation of PROX1 by reducing hsa-miR-423-5p

et al. 2019

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“…Unexpectedly, some miRNAs are destabilized by specific interactions with mRNAs [68,69]. These transcripts contain sequences that have a near-perfect match with miRNAs and contain centered mismatches.…”

Section: Introductionmentioning

confidence: 99%

The Non-Canonical Aspects of MicroRNAs: Many Roads to Gene Regulation

Stavast

Erkeland

2019

Cells

293

207

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MicroRNAs (miRNAs) are critical regulators of gene expression. As miRNAs are frequently deregulated in many human diseases, including cancer and immunological disorders, it is important to understand their biological functions. Typically, miRNA-encoding genes are transcribed by RNA Polymerase II and generate primary transcripts that are processed by RNase III-endonucleases DROSHA and DICER into small RNAs of approximately 21 nucleotides. All miRNAs are loaded into Argonaute proteins in the RNA-induced silencing complex (RISC) and act as post-transcriptional regulators by binding to the 3′- untranslated region (UTR) of mRNAs. This seed-dependent miRNA binding inhibits the translation and/or promotes the degradation of mRNA targets. Surprisingly, recent data presents evidence for a target-mediated decay mechanism that controls the level of specific miRNAs. In addition, several non-canonical miRNA-containing genes have been recently described and unexpected functions of miRNAs have been identified. For instance, several miRNAs are located in the nucleus, where they are involved in the transcriptional activation or silencing of target genes. These epigenetic modifiers are recruited by RISC and guided by miRNAs to specific loci in the genome. Here, we will review non-canonical aspects of miRNA biology, including novel regulators of miRNA expression and functions of miRNAs in the nucleus.

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MicroRNA target prediction tools for animals: Where we are at and where we are going to - A systematic review

Feitosa

Prieto-Oliveira

Brentani

et al. 2022

Computational Biology and Chemistry

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Non-canonical targets play an important role in microRNA stability control mechanisms

Cited by 4 publications

References 0 publications

RETRACTED ARTICLE: Long non-coding RNA LINC00968 reduces cell proliferation and migration and angiogenesis in breast cancer through up-regulation of PROX1 by reducing hsa-miR-423-5p

RETRACTED ARTICLE: Long non-coding RNA LINC00968 reduces cell proliferation and migration and angiogenesis in breast cancer through up-regulation of PROX1 by reducing hsa-miR-423-5p

The Non-Canonical Aspects of MicroRNAs: Many Roads to Gene Regulation

MicroRNA target prediction tools for animals: Where we are at and where we are going to - A systematic review

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