Non-canonical Ca2+- Akt signaling pathway mediates the antiproteolytic effects induced by oxytocin receptor stimulation in skeletal muscle

Santos, Tatiane de Oliveira; Cruz-Filho, João da; Costa, Daniely Messias; Silva, Raquel Prado da; Anjos-Santos, Hevely Catharine dos; Santos, José Ronaldo dos; Reis, Luís Carlos; Kettelhut, Ísis do Carmo; Navegantes, Luiz Carlos; Camargo, Enilton Aparecido; Lauton-Santos, Sandra; Badauê-Passos Jr, Daniel; Mecawi, André de Souza; Lustrino, Danilo

doi:10.1016/j.bcp.2023.115850

Cited by 3 publications

(1 citation statement)

References 49 publications

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“…Soleus muscles were collected, attached to specific supports, and incubated for 1 h in 3 mL of Krebs‐Ringer bicarbonate buffer (pH 7.4, 5 mM glucose and 0.5 mM cycloheximide, equilibrated with 95% O 2 and 5% CO 2 ). Subsequently, the solution was replaced with the same volume of Krebs buffer and incubated for 2 h 62 . To determine the overall proteolysis rate, 1 mL of buffer was collected to quantify tyrosine release into the solution 55 using fluorimetry 63 .…”

Section: Methodsmentioning

confidence: 99%

Adrenalectomy attenuates hyperalgesia but does not regulate muscle wasting in a female rat model of fibromyalgia

Costa,

da Silva,

da Cruz‐Filho

et al. 2024

Clin Exp Pharma Physio

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Although it is well established that fibromyalgia (FM) syndrome is characterized by chronic diffuse musculoskeletal hyperalgesia, very little is known about the effect of this pathology on muscle tissue plasticity. Therefore, the present study aimed to characterize the putative alterations in skeletal muscle mass in female rats subjected to a FM model by inducing chronic diffuse hyperalgesia (CDH) through double injections of acidic saline (pH 4.0) into the left gastrocnemius muscle at 5‐day intervals. To determine protein turnover, the total proteolysis, proteolytic system activities and protein synthesis were evaluated in oxidative soleus muscles of pH 7.2 (control) and pH 4.0 groups at 7 days after CDH induction. All animals underwent behavioural analyses of mechanical hyperalgesia, strength and motor performance. Our results demonstrated that, in addition to hyperalgesia, rats injected with acidic saline exhibited skeletal muscle loss, as evidenced by a decrease in the soleus fibre cross‐sectional area. This muscle loss was associated with increased proteasomal proteolysis and expression of the atrophy‐related gene (muscle RING‐finger protein‐1), as well as reduced protein synthesis and decreased protein kinase B/S6 pathway activity. Although the plasma corticosterone concentration did not differ between the control and pH 4.0 groups, the removal of the adrenal glands attenuated hyperalgesia, but it did not prevent the increase in muscle protein loss in acidic saline‐injected animals. The data suggests that the stress‐related hypothalamic–pituitary–adrenal axis is involved in the development of hyperalgesia, but is not responsible for muscle atrophy observed in the FM model induced by intramuscular administration of acidic saline. Although the mechanisms involved in the attenuation of hyperalgesia in rats injected with acidic saline and subjected to adrenalectomy still need to be elucidated, the results found in this study suggest that glucocorticoids may not represent an effective therapeutic approach to alleviate FM symptoms.

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Section: Methodsmentioning

confidence: 99%

Adrenalectomy attenuates hyperalgesia but does not regulate muscle wasting in a female rat model of fibromyalgia

Costa,

da Silva,

da Cruz‐Filho

et al. 2024

Clin Exp Pharma Physio

Self Cite

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Reduced sympathetic activity is associated with the development of pain and muscle atrophy in a female rat model of fibromyalgia

da Silva,

Costa,

da Cruz-Filho

et al. 2024

Physiology & Behavior

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The Pivotal Role of Oxytocin’s Mechanism of Thermoregulation in Prader-Willi Syndrome, Schaaf-Yang Syndrome, and Autism Spectrum Disorder

Camerino

2024

IJMS

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Oxytocin (Oxt) regulates thermogenesis, and altered thermoregulation results in Prader-Willi syndrome (PWS), Schaaf-Yang syndrome (SYS), and Autism spectrum disorder (ASD). PWS is a genetic disorder caused by the deletion of the paternal allele of 15q11-q13, the maternal uniparental disomy of chromosome 15, or defects in the imprinting center of chromosome 15. PWS is characterized by hyperphagia, obesity, low skeletal muscle tone, and autism spectrum disorder (ASD). Oxt also increases muscle tonicity and decreases proteolysis while PWS infants are hypotonic and require assisted feeding in early infancy. This evidence inspired us to merge the results of almost 20 years of studies and formulate a new hypothesis according to which the disruption of Oxt’s mechanism of thermoregulation manifests in PWS, SYS, and ASD through thermosensory abnormalities and skeletal muscle tone. This review will integrate the current literature with new updates on PWS, SYS, and ASD and the recent discoveries on Oxt’s regulation of thermogenesis to advance the knowledge on these diseases.

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Non-canonical Ca2+- Akt signaling pathway mediates the antiproteolytic effects induced by oxytocin receptor stimulation in skeletal muscle

Cited by 3 publications

References 49 publications

Adrenalectomy attenuates hyperalgesia but does not regulate muscle wasting in a female rat model of fibromyalgia

Adrenalectomy attenuates hyperalgesia but does not regulate muscle wasting in a female rat model of fibromyalgia

Reduced sympathetic activity is associated with the development of pain and muscle atrophy in a female rat model of fibromyalgia

The Pivotal Role of Oxytocin’s Mechanism of Thermoregulation in Prader-Willi Syndrome, Schaaf-Yang Syndrome, and Autism Spectrum Disorder

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