Non‐canonical amino acids uncover the significant impact of Tyr671 on TaqDNA polymerase catalytic activity
Wanyi Chen,
Binbin Chen,
Xinjia Li
et al.
Abstract:Responsible for synthesizing the complementary strand of the DNA template, DNA polymerase is a crucial enzyme in DNA replication, recombination and repair. A highly conserved tyrosine (Tyr), located at the C‐terminus of the O‐helix in family A DNA polymerases, plays a critical role in enzyme activity and fidelity. Here, we combined the technology of genetic code extension to incorporate non‐canonical amino acids and molecular dynamics (MD) simulations to uncover the mechanisms by which Tyr671 impacts substrate… Show more
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