Non-bonded poly(ethylene oxide) polymer-coated column for protein separation by capillary electrophoresis

Iki, Nobuhiko; Yeung, Edward S.

doi:10.1016/0021-9673(95)01158-7

Cited by 166 publications

(149 citation statements)

References 40 publications

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“…However, the procedure requires the use of an organic solvent and careful control of the experimental conditions. More hydrophobic polymers, such as poly(vinylpyrrolidone), poly(dimethylacrylamide) (poly(DMA)), or poly(ethylene oxide) [17], possess a higher affinity for the surface; therefore, once adsorbed on the capillary wall, they form more stable coatings that are not easily removed by any aqueous buffer. Although useful in many applications, these coatings are not recommended for proteins.…”

Section: Introductionmentioning

confidence: 99%

New adsorbed coatings for capillary electrophoresis

et al. 2000

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Section: Introductionmentioning

confidence: 99%

New adsorbed coatings for capillary electrophoresis

et al. 2000

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“…1(b)). Though it was previously reported that the coated capillary with only HEC or PEG 8000000 via hydrogen bonding between polymers and protonated silanols on the surface of the capillary inner wall was stable only in weakly acidic conditions (below pH 6), 17 the results of this work showed that the capillary coating was stable in neutral conditions, and EOF was completely eliminated at pH 7.0 by employing the mixed polymer. The procedure for capillary coating in our previous method was complicated and took at least 3 h. The procedure was drastically simplified in the proposed method as the coating is achieved just by flushing the electrophoretic buffer solution after washing with HCl solution for a few minutes.…”

Section: Dynamic Coating Ce With Non-ionic Polymers With Hydrogen Bonmentioning

confidence: 55%

“…17 The polymer was immobilized on the capillary inner wall via hydrogen bonding to the surface silanol groups by flushing the capillary with an acidic aqueous polymer solution after filling the capillary with HCl solution. While this procedure is quite simple, there is a problem that the coated capillary is only use in acidic and neutral conditions.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Coating Capillary Electrophoresis for Separation of Humic Acid Using Mixture Solution of Non-ionic Polymers both as Coating Agent and Separation Medium

et al. 2013

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A simple, rapid, and precise dynamic coating capillary electrophoretic separation method for water-soluble humic substances is proposed. An aqueous solution containing hydroxyethyl cellulose (HEC), polyethylene glycol (PEG), and pH buffer component was employed for both the dynamic coating agent and the separation medium. The procedure for the coating of the capillary inner wall was simply filling the buffered polymer mixture solution into the capillary that had been treated with 1 M aqueous HC1 solution. The solution for the capillary coating was directly used as the electrophoretic buffer solution for CE separation. Excellent performance for the separation of humic acid was obtained using the solution containing 0.5% (w/v) HEC, 1.0% (w/v) PEG 10000, and 0.1% (w/v) PEG 8000000. Excellent reproducibility and durability were obtained even at slightly alkaline conditions at pH levels above 8. The separation of 0.1 - 2 kbp of DNA ladder by the proposed method showed was also achieved

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“…2. When a bare capillary was used to separate separate basic proteins [25] was employed in prothe sample of PEGylated RNase, it gave out two cedure B (Fig. 2B).…”

Section: Preparation Of Peg-proteinsmentioning

confidence: 99%

“…In the experiments, we used a similar procedure to that described by Iki et al [25] to make a solution of 6 PEO 8310 as the agent for dynamic coating. PEO 6 …”

Section: Introductionmentioning

confidence: 99%