Non-beating HL-1 cells for confocal microscopy: Application to mitochondrial functions during cardiac preconditioning

Pelloux, Sophie; Robillard, Julie M.; Ferrera, René; Bilbaut, André; Ojeda, Carlos; Saks, Valdur; Ovize, Michel; Tourneur, Yves

doi:10.1016/j.pbiomolbio.2005.06.009

Cited by 41 publications

(35 citation statements)

References 66 publications

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“…The model works with absolute currents. Estimating the radius of an HL-1 cell to be 7 μm [47] and knowing the electrode opening radius of 2.5 μm we assume χ total ≈ 5% of the total current I total through the membrane to be important for the signal…”

Section: A Propagation Of Action Potentialsmentioning

confidence: 99%

Frequency-dependent signal transfer at the interface between electrogenic cells and nanocavity electrodes

et al. 2012

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We present a model to describe the response of chip-based nanocavity sensors during extracellular recording of action potentials. These sensors feature microelectrodes which are embedded in liquid-filled cavities. They can be used for the highly localized detection of electrical signals on a chip. We calculate the sensor's impedance and simulate the propagation of action potentials. Subsequently we apply our findings to analyze cell-chip coupling properties. The results are compared to experimental data obtained from cardiomyocyte-like cells. We show that both the impedance and the modeled action potentials fit the experimental data well. Furthermore, we find evidence for a large seal resistance of cardiomyocytes on nanocavity sensors compared to conventional planar recording systems.

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Section: A Propagation Of Action Potentialsmentioning

confidence: 99%

Frequency-dependent signal transfer at the interface between electrogenic cells and nanocavity electrodes

et al. 2012

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“…The optical control of confocal microscopy lasers would allow pulses of various intensities of light to be applied in a suppression regime interspersed with 10 optical measurements. Laser stimulation could conversely be used to activate model systems that do not normally produce reliable APs [46] whereas ChR2 gives the advantage of artifact free and reliable stimulation without permeabilization. Nevertheless, electric pacemakers are commonly used and well explored.…”

Section: Applicationmentioning

confidence: 99%

Light induced stimulation and delay of cardiac activity

et al. 2010

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“…We studied in confocal microscopy non-beating cells [1] from the first cardiac cell line, HL-1, kindly provided by Dr W. Claycomb [2]. Cells were incubated with a mitochondrial selective fluorescent dye, a rhodamine derivative, TMRM (Molecular Probes, Eugene, OR) for 15 min before the experiment.…”

Section: Methodsmentioning

confidence: 99%