Non-Antarctic notothenioids: Past phylogenetic history and contemporary phylogeographic implications in the face of environmental changes

Papetti, Chiara; Windisch, Heidrun Sigrid; Mesa, Mario La; Lucassen, Magnus; Marshall, Craig J.; Lamare, Miles D.

doi:10.1016/j.margen.2015.11.007

Cited by 13 publications

(13 citation statements)

References 87 publications

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“…This would effectively help monitoring the genetic structure of the three Lepidonotothen species, profiling demographic events occurred in the past, and identifying signatures of local adaptation in genetically different populations. To understand the significance of these events and the climate challenges that marine organisms in polar regions are facing, is essential to model and project future population viability for species management and conservation [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Microsatellite markers for the notothenioid fish Lepidonotothen nudifrons and two congeneric species

et al. 2016

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BackgroundLoss of genetic variability due to environmental changes, limitation of gene flow between pools of individuals or putative selective pressure at specific markers, were previously documented for Antarctic notothenioid fish species. However, so far no studies were performed for the Gaudy notothen Lepidonotothen nudifrons. Starting from a species-specific spleen transcriptome library, we aimed at isolating polymorphic microsatellites (Type I; i.e. derived from coding sequences) suitable to quantify the genetic variability in this species, and additionally to assess the population genetic structure and demography in nototheniids.ResultsWe selected 43,269 transcripts resulting from a MiSeq sequencer run, out of which we developed 19 primer pairs for sequences containing microsatellite repeats. Sixteen loci were successfully amplified in L.nudifrons. Eleven microsatellites were polymorphic and allele numbers per locus ranged from 2 to 17. In addition, we amplified loci identified from L. nudifrons in two other congeneric species (L. squamifrons and L. larseni). Thirteen loci were highly transferable to the two congeneric species. Differences in polymorphism among species were detected.ConclusionsStarting from a transcriptome of a non-model organism, we were able to identify promising polymorphic nuclear markers that are easily transferable to other closely related species. These markers can be a key instrument to monitor the genetic structure of the three Lepidonotothen species if genotyped in larger population samples. When compared with anonymous loci isolated in other notothenioids, i.e. Type II (isolated from genomic libraries), they offer the possibility to test how the effects of occurring environmental change influence the population genetic structure in each species and subsequently the composition of the entire ecosystem.

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Section: Resultsmentioning

confidence: 99%

Microsatellite markers for the notothenioid fish Lepidonotothen nudifrons and two congeneric species

et al. 2016

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“… Cladogram and chromosome numbers for Antarctic fish. The phylogeny is modified from ( Near 2008 ; Near 2015 ; Near 2004 ; Papetti 2016 ). Abbreviations: 2 N , diploid chromosome number; NF, the number of chromosome arms (nombre fundamental).…”

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confidence: 99%

Cold Fusion: Massive Karyotype Evolution in the Antarctic Bullhead NotothenNotothenia coriiceps

Amores

Wilson

Allard

et al. 2017

G3 Genes|Genomes|Genetics

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Half of all vertebrate species share a series of chromosome fusions that preceded the teleost genome duplication (TGD), but we do not understand the causative evolutionary mechanisms. The “Robertsonian-translocation hypothesis” suggests a regular fusion of each ancestral acro- or telocentric chromosome to just one other by centromere fusions, thus halving the karyotype. An alternative “genome-stirring hypothesis” posits haphazard and repeated fusions, inversions, and reciprocal and nonreciprocal translocations. To study large-scale karyotype reduction, we investigated the decrease of chromosome numbers in Antarctic notothenioid fish. Most notothenioids have 24 haploid chromosomes, but bullhead notothen (Notothenia coriiceps) has 11. To understand mechanisms, we made a RAD-tag meiotic map with ∼10,000 polymorphic markers. Comparative genomics aligned about a thousand orthologs of platyfish and stickleback genes along bullhead chromosomes. Results revealed that 9 of 11 bullhead chromosomes arose by fusion of just two ancestral chromosomes and two others by fusion of three ancestral chromosomes. All markers from each ancestral chromosome remained contiguous, implying no inversions across fusion borders. Karyotype comparisons support a history of: (1) Robertsonian fusions of 22 ancestral chromosomes in pairs to yield 11 fused plus two small unfused chromosomes, like N. angustata; (2) fusion of one of the remaining two ancestral chromosomes to a preexisting fused pair, giving 12 chromosomes like N. rossii; and (3) fusion of the remaining ancestral chromosome to another fused pair, giving 11 chromosomes in N. coriiceps. These results raise the question of what selective forces promoted the systematic fusion of chromosomes in pairs and the suppression of pericentric inversions in this lineage, and provide a model for chromosome fusions in stem teleosts.

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“…Papetti et al [2] assessed the GenBank information for E. maclovinus, finding 125 nucleotide entries (71 mitochondrial, 54 nuclear, with 10 microsatellite markers among these), 124 protein entries (78 mitochondrial, 46 nuclear), and 38 popsets (phylogenetic studies). The sequences reported by the present study provide a new source of genetic information that can be used in future studies dealing with immunological response of E. maclovinus and other notothenioids Antarctic at a comparative level.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, these families are known as non-Antarctic Notothenioidei that diverged early from the main notothenioid lineage. These families also never established on the Antarctic shelf [2], either due to a lack of crucial biochemical and physiological adaptations to cold (e.g., antifreeze glycoproteins), the lack of dispersal potential (e.g., small eggs and larval stages with reduced mobility), or the dependence on estuarine environments that are absent in the Antarctic [3]. Meanwhile, the Antarctic fish lack a common cellular defense mechanism called the heat shock response (HSR) by molecular chaperones [4], developing a cardiovascular system with modifications that involve large-bore blood vessels, large blood volumes; and large hearts with high content of mitochondria [5].…”

Section: Introductionmentioning

confidence: 99%

“…To date, relatively little sequence information is available for E. maclovinus [2], despite the physiological characteristics that consider it an eurythermic, euryhaline and stenobatic species [14], with a hermaphrodite type of reproduction (unique among the notothenioids) [15] and with a relevant participation in the transmission of diseases that affect aquaculture as the Piscirickettsiosis [16]. This report describes the de novo assembly and annotation of the E. maclovinus head kidney transcriptome, providing a new source of genetic information that can be used in future studies dealing with the immunological response of notothenioids.…”

Section: Introductionmentioning

confidence: 99%

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Head Kidney Transcriptome Analysis and Characterization for the Sub-Antarctic Notothenioid Fish Eleginops maclovinus

Martínez

Pontigo

Morera

et al. 2018

Fishes

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This study describes de novo transcriptome sequencing and annotation analyses for the head kidney of the sub-Antarctic notothenioid fish Eleginops maclovinus, a sister group of the Antarctic notothenioid fish clade. Moreover, E. maclovinus is one of the most eurythermal and euryhaline representatives of the Notothenioidei suborder. RNA-seq data were generated by the 454 GS Junior system, resulting in 11,207 contigs that were then assembled by the Genomic Workbench CLC software. The transcriptome was annotated by BLASTing each sequence against the universal, non-redundant NCBI database (National Center for Biotechnology Information) using the AUSTRAL-omics computer cluster. A significant number of transcripts related to innate and adaptive immunity were found in the sequences, which could be used as references in future immunological studies in E. maclovinus.

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Non-Antarctic notothenioids: Past phylogenetic history and contemporary phylogeographic implications in the face of environmental changes

Cited by 13 publications

References 87 publications

Microsatellite markers for the notothenioid fish Lepidonotothen nudifrons and two congeneric species

Microsatellite markers for the notothenioid fish Lepidonotothen nudifrons and two congeneric species

Cold Fusion: Massive Karyotype Evolution in the Antarctic Bullhead NotothenNotothenia coriiceps

Head Kidney Transcriptome Analysis and Characterization for the Sub-Antarctic Notothenioid Fish Eleginops maclovinus

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