Non-Animal Stabilized Hyaluronic Acid

Abstract

Hyaluronic acid (HA) is a major component of human synovial fluid, providing the rheologic properties (elasticity and viscosity) that enable the synovial fluid to perform lubricating and shock-absorbing functions within the healthy joint. Over the last 2 decades, HA preparations have become established in intra-articular therapy of osteoarthritis (OA), particularly OA of the knee. Existing HA preparations, both cross-linked and non-cross-linked, are all administered by courses of multiple injections, and all have been associated with variable success rates. The clinical profile of an HA preparation is inextricably linked to the product's physicochemical properties. For example, the molecular structure of the HA affects the intra-articular residence time, which should in turn influence the duration of action post-injection. Non-animal stabilized hyaluronic acid (NASHA) is a new-generation HA preparation, produced wholly from non-animal sources. NASHA is stabilized using a carefully controlled cross-linking process, which increases the intra-articular residence time from hours to weeks. This facilitates single-injection treatment for OA without affecting the biocompatibility of HA. This review evaluates the properties of NASHA, including the available clinical data, in the context of previously developed HA preparations.

Injection of Stabilized Hyaluronic Acid-Based Gel of Non-Animal Origin for the Correction of Nasolabial Folds

“…Although NASHA‐based gel is biocompatible, facilitating an excellent safety profile, it is also biodegradable 19 . The new NASHA‐based gel containing lidocaine has the same chemical and physical characteristics (gel content, particle size, pH, and degree of modification) as NASHA‐based gel without lidocaine.…”

Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco‐supplementation in Orthobiologics

“…[254,255] The average protein content of hylan A is 0.4-0.8%. [257] NASHA are new-generation HAs with improved biophysical properties compared to unmodified HA. NASHA (nonanimal stabilized HA) was subsequently developed to provide a high molecular weight cross-link stabilized HA preparation from a bacterial ferment to avoid any immunological sensitivity issues which have been reported for animal sourced HAs.…”

“…NASHA (nonanimal stabilized HA) was subsequently developed to provide a high molecular weight cross-link stabilized HA preparation from a bacterial ferment to avoid any immunological sensitivity issues which have been reported for animal sourced HAs. [257] This size imparts important beneficial therapeutic properties to NASHA in terms of its rheological and visco-elastic performance as a visco-supplement Adv Dermal implantation for aesthetic use in the hands mid-deep dermal injection for aesthetic correction of moderate to severe facial wrinkles and folds in adults >21 years old [276] 1. NASHA injections deliver an increased density of HA and display a high resistance to intraarticular degradation increasing its intra-articular residency time and clinical effect.…”

“…Biomedical B Baumann Group [242] 10 × 10 12 Da NASHA HAs Durolane Bioventus (USA) [243,257,268,269] functionalities which are reactive with bi-functional reagents and these can be used to attach peptides to the CS backbone. AFM confirmed that the attached peptides adopted a bristle-like distribution in a bottle-brush type arrangement on the CS core and they thus explored all available spatial orientations which would be expected to optimize the space-filling and interactive properties of the peptidoglycans.…”

Hyaluronic acid alkyl derivative: A novel inhibitor of metalloproteases and hyaluronidases

“…Currently, all of the polymers that are used in OA intra-articular therapy are based on chondroitin sulphate (CS) [26], high MW HA or HA that is chemically modified either by cross-linking (mainly with BDDE [27] or DVS [28]) or by amidation of the carboxylic groups with an alkyl chain [22]. To determine whether some of these have an inhibitory effect on MMPs, we synthesized the modified HA polymers (HBC, HDC, Hyadd) and monitored their effect on the enzymatic activity in an MMP collagenase (ChC) model [24].…”