Nomenclature for factors of the BoLA system, 1996: report of the ISAG BoLA Nomenclature Committee

Davies, Christopher J.; Andersson, Lars; Mikko, Sofia; Ellis, Shirley A.; Hensen, E. J.; Lewin, H A; Muggli‐Cockett, N. E.; Poel, J.J. van der; Russell, George C.

doi:10.1111/j.1365-2052.1997.00106.x

Cited by 100 publications

(54 citation statements)

References 16 publications

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“…These haplotypes share both a serological specificity, Dw3 (28), and an allo-T cell specificity (42), yet all have different DR molecules (28). In addition, the DQB*0103 allele, associated with DH28A by RFLP typing data (28), differs from the DH24A DQB*0101 allele by only four conservative substitutions in the Ag-binding cleft (Y30-H, F61-W, F67-I, and Q70-R) (43). Thus, the DQ molecules of DH24A/DH15B and DH28A may belong to a class II supertype (44), sharing structural similarities that are reflected in both their peptide and TCR specificity.…”

Section: Discussionmentioning

confidence: 99%

Duplicated DQ Haplotypes Increase the Complexity of Restriction Element Usage in Cattle

Glass

Oliver

Russell

2000

The Journal of Immunology

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The MHC of cattle encodes two distinct isotypes of class II molecules, DR and DQ. Unlike humans, cattle lack the DP locus and about half the common haplotypes express duplicated DQ genes. The number and frequency of DQA and DQB alleles means that most cattle are heterozygous. If inter- and/or intrahaplotype pairing of DQA and DQB molecules occurs, cattle carrying DQ-duplicated haplotypes may express more restriction elements than would be predicted by the number of expressed alleles. We are investigating whether duplicated haplotypes cause differences in immune response, particularly in terms of generating protective immunity. We have analyzed the Ag-presenting function of DQ molecules in two heterozygous animals, one of which carries a duplicated haplotype. We compared the class II isotype specificity of T cell clones recognizing a putative vaccinal peptide from foot-and-mouth disease virus (FMDV15). We show for the first time that bovine T cells can recognize Ag in the context of DQ molecules. We also present evidence that interhaplotype pairings of DQA and DQB molecules form functional restriction elements. Both animals showed distinct biases to usage of particular restriction elements. Mainly DQ-restricted clones were derived from the animal with duplicated DQ genes, whereas the majority of clones from the animal with a single DQ gene pair were DR restricted. Furthermore, haplotype bias was observed with both animals. These experiments show that understanding of class II chain pairing in addition to knowledge of the genotype may be important in vaccine design where effective epitope selection is essential.

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Section: Discussionmentioning

confidence: 99%

Duplicated DQ Haplotypes Increase the Complexity of Restriction Element Usage in Cattle

Glass

Oliver

Russell

2000

The Journal of Immunology

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“…Three of the class II haplotypes carried by these cattle (DH8A, DH16A, and DH22C) have duplicated DQA and DQB genes; the fourth haplotype (DH24A) has only one DQA and one DQB gene (3,12). The BoLA-DQ haplotypes of the cows were inferred from BoLA-A and DRB3 typing on the basis of haplotypes defined in the Fifth and Seventh International BoLA Workshops (11,12,26,38) (BoLA nomenclature website [http://www.projects.roslin.ac.uk/bolahome.html]).…”

Section: Cd8mentioning

confidence: 99%

Neospora caninum-Infected Cattle Develop Parasite-Specific CD4⁺Cytotoxic T Lymphocytes

et al. 2003

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Cattle infected with Neospora caninum readily experience transplacental parasite transmission, presumably after maternal parasitemia, leading to abortion or birth of congenitally infected calves. Cytotoxic T lymphocytes (CTL) are important mediators of protective immunity against Toxoplasma gondii, an intracellular apicomplexan protozoan closely related to N. caninum. In this study, N. caninum-specific CTL expanded from peripheral blood mononuclear cells of two major histocompatibility complex-mismatched, experimentally infected cattle were identified by using a 51 Cr release cytotoxicity assay. Enrichment and blocking of CD4 ؉ -and CD8 ؉ -T-lymphocyte effector subsets indicated that CD4 ؉ CTL killed N. caninum-infected, autologous target cells and that killing was mediated through a perforin/granzyme pathway. Detection and characterization of CTL responses to N. caninum in the natural, outbred, bovine host will facilitate identification of immunogens and design of immunization strategies to induce parasite-specific CTL against transplacental N. caninum transmission in cattle.

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“…Statistical analyses were carried out using SPSS 13.0 (IBM, Armonk, NY, USA) and polymorphic value analysis was performed using DnaSP 4.0 [38]. Novel alleles, as determined by the deduced amino acid sequences, were designated Scma-DAB*0101-Scma-DAB* 1601 according to the rules proposed by Davies et al [39]. The abbreviation Scma refers to Scophthalmus maximus, D to Class II, A to uncharacterized family designation, and B to the β chain-encoding genes.…”

Section: Amino Acid Sequence Analysis and Statistical Testsmentioning

confidence: 99%

MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families

Chen

Liu

et al. 2012

Chin. Sci. Bull.

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This study examined genetic variation in the major histocompatibility complex (MHC) Class II B gene in turbot (Scophthalmus maximus) by virulent bacterial pathogen challenge. One hundred fry from each of six families were infected with Edwardsiella tarda by intraperitoneal injection. Family mortality ranged from 28.0% to 83.3%. Complete exon 2 and intron 1 sequences of MHC Class II B genes were amplified from five survivor and five non-survivor individuals per family using the clone-sequence method. Thirty-seven sequences from 60 individuals revealed 37 different alleles, 25 of which were unique to this study. The 25 unique alleles belonged to 16 major allele types. Nine alleles were used to examine the association between alleles and resistance/susceptibility to disease. Five alleles were present in an individual, suggesting a minimum of three loci or copies of the turbot MHC Class II B gene. The rate of non-synonymous substitution (d N ) was 2.30 and 1.58 times higher than synonymous substitution (d S ) in the peptide-binding regions (PBR) and non-PBR in whole families, respectively, which suggested balancing selection on exon 2 of the MHC Class II B gene in turbot. One allele, Scma-DBB1*02, was significantly more prevalent in survivor stock than in non-survivor stock (P=0.001). Therefore, this allele might be associated with resistance to bacteria. A second allele, Scma-DBB1*10, was significantly more prevalent in non-survivor stock (P=0.021), and is likely associated with susceptibility to bacteria. turbot (Scophthalmus maximus), Edwardsiella tarda, major histocompatibility complex II B, polymorphism, resistance, susceptibility

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Nomenclature for factors of the BoLA system, 1996: report of the ISAG BoLA Nomenclature Committee

Cited by 100 publications

References 16 publications

Duplicated DQ Haplotypes Increase the Complexity of Restriction Element Usage in Cattle

Duplicated DQ Haplotypes Increase the Complexity of Restriction Element Usage in Cattle

Neospora caninum-Infected Cattle Develop Parasite-Specific CD4⁺Cytotoxic T Lymphocytes

MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families

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Nomenclature for factors of the BoLA system, 1996: report of the ISAG BoLA Nomenclature Committee

Cited by 100 publications

References 16 publications

Duplicated DQ Haplotypes Increase the Complexity of Restriction Element Usage in Cattle

Duplicated DQ Haplotypes Increase the Complexity of Restriction Element Usage in Cattle

Neospora caninum-Infected Cattle Develop Parasite-Specific CD4+Cytotoxic T Lymphocytes

MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families

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Neospora caninum-Infected Cattle Develop Parasite-Specific CD4⁺Cytotoxic T Lymphocytes