“Nojirimycin” as a Potent Inhibitor of Glucosidase

NIWA, Tomizo; INOUYE, Sigeharu; TSURUOKA, Takashi; Koaze, Yoshihisa; NIIDA, Taro

doi:10.1271/bbb1961.34.966

Cited by 101 publications

(34 citation statements)

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“…The induction medium in 50 mM potassium phosphate buffer (pH 6.0) contained the following (in grams per liter): (NH4)2SO4, 1.4; MgSO4. 7H20, 0.3; Tween 80, 2.0; and sugar to be tested, (20), was added to retard degradation of the inducer. Unless otherwise stated, the concentration of nojirimycin was 10 ,ug/ml.…”

Section: Methodsmentioning

confidence: 99%

Induction of Cellulase by Gentiobiose and Its Sulfur-Containing Analog in Penicillium purpurogenum

Kurasawa

Yachi

Suto

et al. 1992

Appl Environ Microbiol

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Cellulase induction by 3-glucodisaccharides was investigated by using non-cellulase-induced mycelia of Penicillium purpurogenum P-26, a highly-cellulase-producing fungus. Gentiobiose induced significant amounts of cellulase compared with cellobiose when nojirimycin was added to the induction medium to inhibit extracellular I8-glucosidase activity. Thiogentiobiose (6-S-4-D-glucopyranosyl-6-thio-D-glucose), a sulfur-containing analog of gentiobiose, was more effective for cellulase induction than gentiobiose even in the absence of nojirimycin. Thiogentiobiose appeared to be a gratuitous inducer since it was not metabolized during cellulase induction. Gentiobiose was formed from cellobiose by the intracellular j8-glucosidase of P. purpurogenum. These findings indicate that gentiobiose is an active inducer of cellulase for this fungus and may possibly be formed by intracellular ,1-glucosidase from cellobiose.

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Section: Methodsmentioning

confidence: 99%

Induction of Cellulase by Gentiobiose and Its Sulfur-Containing Analog in Penicillium purpurogenum

Kurasawa

Yachi

Suto

et al. 1992

Appl Environ Microbiol

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“…It was the first 5-aminosugar found in nature (Inouye et al, 1968), and is a glucose-analog that has a nitrogen atom instead of oxygen in the pyranose ring. Nojirimycin inhibits both and ~ glucosidases of non-mammalian origin but is a more potent inhibitor of the latter (Niwa et al, 1970;Reese et al, 1971). The amino sugar is a competitive inhibitor of the…”

Section: Nojirimycin (5-amino-5-deoxy-d-glucopyranose)mentioning

confidence: 99%

“…Like the parent compound, 1-deoxynojirimycin is a potent inhibitor of several intestinal ~-glucosidases: sucrase, maltase, isomaltase, glucoamylase (Schmidt et al, 1979). But, contrary to nojirimycin, it is only a weak inhibitor of fl-glucosidases of non-mammalian origin (Niwa et al, 1970;Legler and Jfilich, 1984). Both nojirimycin and l-deoxynojirimycin are fully competitive inhibitors of small intestinal sucrase.…”

Section: -Deoxynojirimycinmentioning

confidence: 99%

Inhibitors of protein glycosylation and glycoprotein processing in viral systems

Datema

Olofsson²,

Romero

1987

Pharmacology & Therapeutics

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“…However, we predicted the presence of some intermediates from DNJ biosynthetic pathway, such as nojirimycin (NJ) and 2-amino-2-deoxy-d-mannitol (ADM) (49). NJ has been identified as iminosugar and is capable in exhibiting an a-glucosidase inhibitory activity (50). Putting these factors into consideration, it is also necessary to investigate the contribution of the non-DNJ components in CBP towards the physiological changes that occur in the present study.…”

Section: Resultsmentioning

confidence: 94%

Physiological Effects and Organ Distribution of <i>Bacillus amyloliquefaciens</i> AS385 Culture Broth Powder Containing 1-Deoxynojirimycin in C57BL/6J Mice

Parida

Takasu

Ito

et al. 2019

J Nutr Sci Vitaminol

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1-Deoxynojirimycin (DNJ) has been known as a potent a-glucosidase inhibitor from mulberry leaves and considered beneficial in prevention of type 2 diabetes. Due to limited amount of DNJ in mulberry leaves, recent studies have focused in finding alternative source that can produce higher amount of DNJ. Previously, we produced a high DNJ-containing culture medium from Bacillus amyloliquefaciens AS385 and constructed a concentration method of bacterial culture medium using cation exchange column. However, less complicated concentration procedure is necessary to save time and cost during the large-scale production. Therefore, we developed a simpler concentration method using anion exchange resin to yield B. amyloliquefaciens AS385 culture broth powder (CBP; 1% DNJ) and evaluated the physiological effects of 5-wk dietary CBP intake in C57BL/6J mice. CBP intake tended to suppress the elevation of blood glucose level during oral glucose tolerance test. Moreover, CBP intake significantly lowered the fasting plasma glucose level and white adipose tissue mass. Next, we evaluated the absorption and distribution of DNJ in mice organs after daily CBP intake. We found detectable amount of DNJ in organs with intestine and kidney as the major targeted organs. We concluded that the DNJ content in CBP is absorbed from digestive tract, distributed and accumulated in organs, which most likely to contribute to the alteration of blood glucose regulation and adiposity in C57BL/6J mice. Our study was the first to report the physiological effects of CBP produced from B. amyloliquefaciens AS385 and the organ distribution of DNJ from CBP.

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“Nojirimycin” as a Potent Inhibitor of Glucosidase

Cited by 101 publications

References 0 publications

Induction of Cellulase by Gentiobiose and Its Sulfur-Containing Analog in Penicillium purpurogenum

Induction of Cellulase by Gentiobiose and Its Sulfur-Containing Analog in Penicillium purpurogenum

Inhibitors of protein glycosylation and glycoprotein processing in viral systems

Physiological Effects and Organ Distribution of <i>Bacillus amyloliquefaciens</i> AS385 Culture Broth Powder Containing 1-Deoxynojirimycin in C57BL/6J Mice

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