Nogo-receptor 1 antagonization in combination with neurotrophin-4/5 is not superior to single factor treatment in promoting survival and morphological complexity of cultured dopaminergic neurons

Seiler, Stefanie; Santo, Stefano Di; Sahli, Sebastian D.; Andereggen, Lukas; Widmer, Hans Rudolf

doi:10.1016/j.brainres.2017.05.015

Cited by 6 publications

(2 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transplanting neural stem cells over expressing NT-3 showed better protective function than grafting neural stem cells alone (Rangasamy et al, 2010). NT-4/5 was reported to promote survival and morphological complexity of cultured dopaminergic neurons (Seiler et al, 2017). Altogether, the anti-oxidant and anti-apoptosis effect of MenSCs-CM on MPP + -injured SH-SY5Y cells may due to the presence of EGF, BDNF, NT-3, and NT-4.…”

Section: Discussionmentioning

confidence: 99%

Conditioned Medium of Human Menstrual Blood-Derived Endometrial Stem Cells Protects Against MPP+-Induced Cytotoxicity in vitro

Han

Yahaya

et al. 2019

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) showed the potential to treat Parkinson’s disease (PD). However, it is unknown whether the conditioned medium of human menstrual blood-derived endometrial stem cells (MenSCs-CM) has the function to alleviate syndromes of PD. In this study, human neuroblastoma SH-SY5Y cells were exposed to neurotoxicant 1-methyl-4-phenylpyridinium (MPP + ) for inducing a range of response characteristics of PD. After culturing this cell model with 24 h/48 h collected MenSCs-CM for different days, cell viability, pro-inflammation cytokines, mitochondrial membrane potential (ΔΨm), oxidative stress, and cell apoptosis were detected. Finally, protein assay was performed to detect 12 kinds of neurotrophic factors inside MenSCs-CM. Our results showed that MPP + caused SH-SY5Y cell viability reduction as an increasing dose and time dependent manner. MPP + treatment resulted in inflammation, mitochondrial dysfunction, reactive oxygen species (ROS) production accumulation, and apoptosis of SH-SY5Y at its IC50 concentration. Forty-eight hours-collected MenSCs-CM and culturing with the MPP + -treated SH-SY5Y for 2 days are the optimized condition to increase cell viability. Besides, MenSCs-CM was efficacious against MPP + induced inflammation, ΔΨm loss, ROS generation, and it could significantly decrease cells numbers in late apoptosis stage. What’s more, protein assay showed that MenSCs-CM contained various neuroprotective factors. Our study provided the first evidence that MenSCs-CM has a protective effect on MPP + -induced cytotoxicity in various aspects, and firstly showed that MenSCs can release at least 12 kinds of neurotrophic factors to medium, which may contribute to the protective function of MenSCs-CM to treat PD. This research enlightening that MenSCs-CM is beneficial in the therapy for PD and probably also for other neurodegenerative diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Conditioned Medium of Human Menstrual Blood-Derived Endometrial Stem Cells Protects Against MPP+-Induced Cytotoxicity in vitro

Han

Yahaya

et al. 2019

Front. Mol. Neurosci.

View full text Add to dashboard Cite

show abstract

“…After 24-h incubation, the primary motoneurons were pre-treated with 0.4 nM recombinant VEGF and inhibitors for 2 h and coincubated with 1 μg/ml lipopolysaccharide (LPS; a neuroinflammation inducer) for 24 h (Herrera et al, 2009 ; Sanchez et al, 2010 ; Seiler et al, 2017 ; Xu et al, 2017 ). After the indicated time points, the neurons were used to perform MTT assay or fixed with 4% PFA for immunofluorescence staining.…”

Section: Methodsmentioning

confidence: 99%

Effect of VEGF on Inflammatory Regulation, Neural Survival, and Functional Improvement in Rats following a Complete Spinal Cord Transection

Chen

Zhao

et al. 2017

Front. Cell. Neurosci.

View full text Add to dashboard Cite

After complete transection of the thoracic spinal segment, neonatal rats exhibit spontaneous locomotor recovery of hindlimbs, but this recovery is not found in adult rats after similar injury. The potential mechanism related to the difference in recovery of neonatal and adult rats remains unknown. In this study, 342 animals were analyzed. The vascular endothelial growth factor (VEGF) level in spinal segments below injury sites was significantly higher in postnatal day 1 rats (P1) compared with 28-day-old adult rats (P28) following a complete T9 transection. VEGF administration in P28 rats with T9 transection significantly improved the functional recovery; by contrast, treatment with VEGF receptor inhibitors in P1 rats with T9 transection slowed down the spontaneous functional recovery. Results showed more neurons reduced in the lumbar spinal cord and worse local neural network reorganization below injury sites in P28 rats than those in P1 rats. Transynaptic tracing with pseudorabies virus and double immunofluorescence analysis indicated that VEGF treatment in P28 rats alleviated the reduced number of neurons and improved their network reorganization. VEGF inhibition in neonates resulted in high neuronal death rate and deteriorated network reorganization. In in vivo studies, T9 transection induced less increase in the number of microglia in the spinal cord in P1 animals than P28 animals. VEGF treatment reduced the increase in microglial cells in P28 animals. VEGF administration in cultured spinal motoneurons prevented lipopolysaccharide (LPS)-induced neuronal death and facilitated neurite growth. Western blots of the samples of lumbar spinal cord after spinal transection and cultured spinal motoneurons showed a lower level of Erk1/2 phosphorylation after the injury or LPS induction compared with that in the control. The phosphorylation level increased after VEGF treatment. In conclusion, VEGF is a critical mediator involved in functional recovery after spinal transection and can be considered a potential target for clinical therapy.

show abstract

Neuroprotection induced by neurotrophic factors

Réus,

Manosso,

Botelho

et al. 2024

Natural Molecules in Neuroprotection and Neurotoxicity

View full text Add to dashboard Cite

Nogo-receptor 1 antagonization in combination with neurotrophin-4/5 is not superior to single factor treatment in promoting survival and morphological complexity of cultured dopaminergic neurons

Cited by 6 publications

References 44 publications

Conditioned Medium of Human Menstrual Blood-Derived Endometrial Stem Cells Protects Against MPP+-Induced Cytotoxicity in vitro

Conditioned Medium of Human Menstrual Blood-Derived Endometrial Stem Cells Protects Against MPP+-Induced Cytotoxicity in vitro

Effect of VEGF on Inflammatory Regulation, Neural Survival, and Functional Improvement in Rats following a Complete Spinal Cord Transection

Neuroprotection induced by neurotrophic factors

Contact Info

Product

Resources

About