Noggin blocks invasive growth of murine B16‐F1 melanoma cells in the optic cup of the chick embryo

Abstract

Melanoma cells originate from the neural crest and are characterized by high migratory potential and invasive growth. After transplantation into the neural tube of the chick embryo, melanoma cells spontaneously emigrate along the neural crest pathways without tumor formation or malignant growth. This emigration depends on the constitutive over-expression of bone morphogenetic protein-2 (BMP-2) and can be ablated by the BMP-antagonist noggin. When transplanted into the embryonic optic cup, melanoma cells invade the host tissue and form malignant tumors. Here, we asked if the invasive growth of melanoma cells in the optic cup could be influenced by BMP-2 or noggin. Mouse B16-F1 cells were grown as aggregates, treated with BMP-2 or noggin during aggregation and transplanted into the optic cup of 3-day chick embryos. After 3 days of subsequent incubation, embryos were evaluated for melanoma cell invasiveness. Immunohistochemical examination revealed that untreated and BMP-2-treated melanoma cells had grown malignantly into the host tissue. However, noggin pretreatment of the aggregates had blocked melanoma cell invasiveness and tumor formation. We conclude that invasive growth of melanoma cells in vivo is BMP-dependent and can be ablated by noggin, thus rendering noggin a promising agent for the treatment of BMP-over-expressing melanoma. ' 2007 Wiley-Liss, Inc.

Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway

“…In our experiments, recombinant human Noggin protein was used as the BMP-2 blocking agent, and it was observed not to affect the high glucose-stimulated BMP-2 expression significantly, but to reduce the production of downstream Cbfα-1 and vascular calcification, correspondingly. This is consistent with the mechanisms of Noggin reported by others (Busch et al, 2008;Takayama et al, 2009). Meanwhile, our results also confirmed that high glucose-induced Cbfα-1 expression was regulated by BMP-2, and high glucose levels might induce osteoblastic differentiation and intracellular calcium deposition via the BMP-2/Cbfα-1 pathway.…”

Resveratrol as a Pan-HDAC Inhibitor Alters the Acetylation Status of Jistone Proteins in Human-Derived Hepatoblastoma Cells

“…For embryotoxicity testing, eggs were used after 50 h of incubation (equal to stage 13 according to Hamburger and Hamilton [39]), which corresponds to approximately six human gestational weeks [40]. The eggs were prepared as described previously [41]. Resveratrol was applied in concentrations of 5, 10, 20, 50 (n = 8) and 100 µM (n = 3) on top of the blastoderm.…”

Exploitation of chick embryo environments to reprogram MYCN-amplified neuroblastoma cells to a benign phenotype, lacking detectable MYCN expression

“…Melanoma cells survive, migrate and/or proliferate quite well when introduced to an inappropriate embryonic environment principally the chick eye cup. 9, 28 Kelly cells did not survive well in the eye cup environment; however, injection of cells at E6 results in the cells targeting major organs principally liver and kidney where they proliferate and form microtumours. The targeting we have observed here is distinctive to MYCN -amplified neuroblastoma cells with cells from other tumours and embryonic stem cells targeting their own distinctive locations.…”