Nodulation of Trifolium subterraneum by introduced rhizobia in competition with naturalized strains

Roughley, R. J.; Blowes, WM; Hurridge, D.F.

doi:10.1016/0038-0717(76)90041-9

Cited by 99 publications

(48 citation statements)

References 12 publications

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“…However, the difference between competitive ability of PDDCC 2163 at the two sites suggests that differences in either the naturalised microflora populations, including rhizobia, or the soil properties, influence competitive ability of strains. A site effect on competitive nodulation of strains was also reported by Roughley et al (1976).…”

Section: Discussionsupporting

confidence: 58%

Competition and persistence of strains ofRhizobium trifoliiin relation to inoculation level and lime pelleting on white clover sown into cultivated soil

Gaur¹,

Lowther²

1982

New Zealand Journal of Agricultural Research

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Five strains of Rhizobium trifolii were assessed for their ability to compete with naturalised strains in nodulating white clover (Trifolium repens) sown in cultivated soil. At the initial assessment 5 months after sowing, the inoculant strains at the normal rate of slurry-inoculation had formed only a small percentage of the nodules present. Increasing the inoculation level to 10 times normal or lime pelleting increased the percentage of nodules formed by all strains apart from PDDCC 2163, which formed a low percentage of nodules irrespective of inoculation and pelleting treatments. The percentage of nodules formed by Strains 2153, 2666, and 2668 declined between the 5-and 18-month assessments so that the percentage of nodules formed by Strain 4144 after 18 months was about twice that of the other strains. Inoculating with Strain 4144 significantly increased %N and total N uptake in clover in spring, 10 months after sowing, compared with the other inoculant strains. However, there were no significant effects on clover dry matter production.

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Section: Discussionsupporting

confidence: 58%

Competition and persistence of strains ofRhizobium trifoliiin relation to inoculation level and lime pelleting on white clover sown into cultivated soil

Gaur¹,

Lowther²

1982

New Zealand Journal of Agricultural Research

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“…It has been observed (Roughley et al 1976) that the inoculum sometimes only occupies a small percentage of nodules when a strong indigenous population is present. The percentage occupancy of nodules by the inoculum strain is shown by genetic analysis (Table 1) to be 28%.…”

Section: Resultsmentioning

confidence: 99%

“…meliloti occupying nodules on lucerne have been shown to comprise a diverse array of types which vary in their frequency of occurrence (Young 1985). The inoculant strains therefore sometimes only occupy a minority of nodules on the host legumes growing in soils containing indigenous populations (Roughley et al 1976). It would therefore be beneficial if more use could be made of the more numerous and possibly, better adapted indigenous rhizobial population for plant nodulation.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of naturally occurringRhizobium melilotipopulations and their potential to form effective symbioses with lucerne

Gandee

Harrison

Davies

1999

Letters in Applied Microbiology

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Genetic characterization by Randomly Amplified Polymorphic DNA (RAPD) fingerprinting was employed to study the status of Rhizobium meliloti populations inhabiting nodules of lucerne. Rhizobium strains were isolated from nodules harvested from plants growing in inoculated or uninoculated experimental plots, uninoculated commercial fields and from lucerne grown in pots containing soils of different origin. Dry matter analyses were recorded and rhizobia were assessed for relative genetic diversity between treatments. Inoculated and uninoculated soils did not differ in terms of dry matter production, and lucerne grew, and was adequately nodulated, in soils with no history of lucerne cultivation. These findings, and the demonstration that there is a rich genetic diversity of Rh. meliloti in these soils, show that it is not always necessary to apply a standard commercial inoculant.

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“…Inability of the inoculant strains to successfully compete with established Rhizobium populations in soil has been frequently observed (Van Rensburg & Strijdom, 1985;Triplett & Sadowsky, 1992). As concluded by Roughley et al (1976) and Vance & Graham (1995), the success of an inoculant decreases with increase of native rhizobia population in soil. Furthermore native rhizobia form the highest number of nodules (Rupela & Sudarshana, 1990).…”

Section: Symbiotic Effectiveness and Ecological Characterization Of Imentioning

confidence: 96%

Symbiotic effectiveness and ecological characterization of indigenous Rhizobium loti populations in Uruguay

Baraibar¹,

Frioni²,

Guedes³

et al. 1999

Pesq. agropec. bras.

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-The objectives of this work were to describe the distribution, density and seasonal variation of the indigenous populations of Rhizobium loti in different Uruguayan soils and to determine the symbiotic effectiveness and stress tolerance factors of different isolates, both with the aim of obtaining selected strains to re-introduce as inoculants in Lotus pastures. R. loti was present in ten soils studied and their densities varied from year to year and within each soil. All the isolates nodulated Lotus corniculatus effectively. The nodules in Lotus pedunculatus and Lotus subbiflorus were small, red on the surface and ineffective in nitrogen fixation. The study of 50 isolates from the ten soils showed high variability in their symbiotic efficiency and tolerance to pH. The indigenous population was acid tolerant in culture medium (pH 4.5), 83% of them could grow at pH 4.5 in 3 days. This work showed that there was a great diversity between the strains of R. loti isolated from Uruguayan soils and supports the importance of selecting among them the most efficient and resistant strains to be included in the inoculants.Index terms: Lotus corniculatus, Lotus pedunculatus, rhizobial pH tolerance.EFICIÊNCIA SIMBIÓTICA E CARACTERIZAÇÃO ECOLÓGICA DE UMA POPULAÇÃO NATIVA DE RHIZOBIUM LOTI NO URUGUAI RESUMO -Os objetivos deste trabalho foram descrever a distribuição, densidade e variação sazonal de populações nativas de Rhizobium loti em diferentes solos uruguaios, e determinar a eficiência simbiótica e os fatores de tolerância a estresse de diversos isolados, tendo em vista a obtenção de cepas a serem reintroduzidas como inoculantes em pastagens de Lotus. Rhizobium loti estava presente em dez solos estudados, e suas densidades variavam de ano para ano e em cada solo. Todos os isolados nodularam efetivamente o Lotus corniculatus. Os nódulos no Lotus pedunculatus e no Lotus subbiflorus eram pequenos, vermelhos na superfície, e ineficazes na fixação de N. O estudo de 50 isolados dos dez solos mostraram alta variabilidade na sua eficiência simbiótica, e alta tolerância ao pH. A população nativa era tolerante à acidez em meio de cultura (pH 4,5); 83% dela pode crescer em pH 4,5 em três dias. O presente trabalho mostrou que há grande diversidade entre as cepas de R. loti isoladas de solos uruguaios, e acentua a importância de se selecionar entre elas as mais eficientes e resistentes, para serem incluídas como inoculantes.Termos para indexação: Lotus corniculatus, Lotus pedunculatus, tolerância do rizóbio ao pH.

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Nodulation of Trifolium subterraneum by introduced rhizobia in competition with naturalized strains

Cited by 99 publications

References 12 publications

Competition and persistence of strains ofRhizobium trifoliiin relation to inoculation level and lime pelleting on white clover sown into cultivated soil

Competition and persistence of strains ofRhizobium trifoliiin relation to inoculation level and lime pelleting on white clover sown into cultivated soil

Genetic characterization of naturally occurringRhizobium melilotipopulations and their potential to form effective symbioses with lucerne

Symbiotic effectiveness and ecological characterization of indigenous Rhizobium loti populations in Uruguay

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