Nodulation, Nutrient Uptake and Yield of Common Bean Inoculated with Rhizobia and Trichoderma in an Acid Soil

Abstract

Common bean is an important source of protein, fat, carbohydrate, vitamins and minerals for both human beings and livestock. In Zambia, common bean is produced mostly by smallholder farmers whose current yields fall far short of the potential 2 t/ha due to various challenges. Among the biophysical constraints, poor soil fertility and acidity pose the greatest challenges. This study investigated the individual and dual inoculation of Rhizobia and Trichoderma to common bean in a phosphorus deficient, acid soil. Soil in which the common bean was grown was characterized for selected chemical properties before planting and at harvest using standard laboratory procedures. Soils were amended with nitrogen and phosphorus fertilizer at the rates of 100 kg N and 80 kg P 2 O 5 per ha; 1 g/kg of seed of Trichoderma harzianum; 100 g/kg of seed of Rhizobium tropici; or a combination of both at the recommended rates at planting. Nodulation and nodule effectiveness were determined at 51 days after planting. Nitrogen and phosphorus accumulation in the above ground biomass, biomass and grain yields were determined at maturity. To determine differences among soil amendments, data were analysed using Analysis of Variance and Least Significant Difference at 95% confidence limit. Relationships among parameters were determined using correlation analysis. The results showed that amending soils with inorganic N at high rates can depress nodulation even in the presence of high levels of inorganic phosphorus. Inoculating common bean with Rhizobia and Trichoderma either singly or in combination increases nodule number and effectiveness per plant but may not result in higher nitrogen and phosphorus accumulation, or in an increase in subsequent biomass or grain yields. The low phosphorus accumulation in Trichoderma inoculated plants observed in this study needs to be investigated further by studying the extent of colonization and the accompanying changes in root volume.

Combined Use of Two Trichoderma Strains to Promote Growth of Pakchoi (Brassica chinensis L.)

“…This indicated that the strain combination had a synergistic effect on the ability of siderophore production. In addition, some Trichoderma strains can promote element solubility (such as phosphorus and potassium) by changing soil fertility [37]. In this study, the LX-7 and HT-1 strains had no ability of phosphate solubilization.…”

Co-inoculation Effect of Rhizobia and Plant Growth Promoting Rhizobacteria on Common Bean Growth in a Low Phosphorus Soil

“…The amount of N per plant was estimated using the correlation derived by Mweetwa et al (2016) relating the number of nodules and the amount of nitrogen accumulated within the common bean plant in an acid soil:…”

Chemical and Biological Properties of Soils Under Maize-Cowpea Cropping Systems in Conservation Agriculture

“…The lower amounts of available phosphorus observed in the cropping sequences with cowpea could be attributed to the fact legumes tend to have a high phosphorus requirement to meet the needs for biological nitrogen fixation resulting in its depletion in the rhizosphere. Phosphorus plays a significant role in determining the energy budget of the symbiosome in the form of ATP (Mweetwa, Chilombo, & Gondwe, 2016). In biological nitrogen fixation, phosphorus is critical for nodule formation and functioning as well as for specific nitrogenase activity (Kouas, Labidi, Debez, & Abdelly, 2005).…”