Nodulation competitiveness of nodule bacteria: Genetic control and adaptive significance: Review

Онищук, О. П.; Vorobyov, N. I.; Проворов, Н. А.

doi:10.1134/s0003683817020132

Cited by 18 publications

(29 citation statements)

References 51 publications

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“…They are represented by rhi genes, which are related to functional groups K (transcription, rhiR), M (cell wall/membrane/envelope biogenesis, rhiD), T (signal transduction mechanisms, rhiI), S (function unknown, rhiB), and other (rhiA, rhiC), and are retained in even the most compact versions of the Sym cluster (3841) and completely absent only in strain Vaf-10 (Table 3). rhi genes are expressed in the rhizosphere and are possibly involved in the pre-infection rhizobia-legume interactions [42], which are essential for nodulation competitiveness [43]. However, no symbiotic defects were previously revealed in the R. leguminosarum mutants for rhi genes [42].…”

Section: Compaction Of the Sym Gene Clustermentioning

confidence: 99%

Search for Ancestral Features in Genomes of Rhizobium leguminosarum bv. viciae Strains Isolated from the Relict Legume Vavilovia formosa

Chirak

Kimeklis

Karasev

et al. 2019

Genes

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Vavilovia formosa is a relict leguminous plant growing in hard-to-reach habitats in the rocky highlands of the Caucasus and Middle East, and it is considered as the putative closest living relative of the last common ancestor (LCA) of the Fabeae tribe. Symbionts of Vavilovia belonging to Rhizobium leguminosarum bv. viciae compose a discrete group that differs from the other strains, especially in the nucleotide sequences of the symbiotically specialised (sym) genes. Comparison of the genomes of Vavilovia strains with the reference group composed of R. leguminosarum bv. viciae strains isolated from Pisum and Vicia demonstrated that the vavilovia strains have a set of genomic features, probably indicating the important stages of microevolution of the symbiotic system. Specifically, symbionts of Vavilovia (considered as an ancestral group) demonstrated a scattered arrangement of sym genes (>90 kb cluster on pSym), with the location of nodT gene outside of the other nod operons, the presence of nodX and fixW, and the absence of chromosomal fixNOPQ copies. In contrast, the reference (derived) group harboured sym genes as a compact cluster (<60 kb) on a single pSym, lacking nodX and fixW, with nodT between nodN and nodO, and possessing chromosomal fixNOPQ copies. The TOM strain, obtained from nodules of the primitive "Afghan" peas, occupied an intermediate position because it has the chromosomal fixNOPQ copy, while the other features, the most important of which is presence of nodX and fixW, were similar to the Vavilovia strains. We suggest that genome evolution from the ancestral to the derived R. leguminosarum bv. viciae groups follows the "gain-and-loss of sym genes" and the "compaction of sym cluster" strategies, which are common for the macro-evolutionary and micro-evolutionary processes. The revealed genomic features are in concordance with a relict status of the vavilovia strains, indicating that V. formosa coexists with ancestral microsymbionts, which are presumably close to the LCA of R. leguminosarum bv. viciae.

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Section: Compaction Of the Sym Gene Clustermentioning

confidence: 99%

Search for Ancestral Features in Genomes of Rhizobium leguminosarum bv. viciae Strains Isolated from the Relict Legume Vavilovia formosa

Chirak

Kimeklis

Karasev

et al. 2019

Genes

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“…Rhizobia are ubiquitous in the soil, their ability to form nodules in the presence of other strains determine their nodulation competitiveness (Onishchuk et al, 2017). In our field experiment, the resident rhizobia were capable of forming root nodules, which is a common situation for this promiscuous crop (Andrews and Andrews, 2017).…”

Section: Competing Ability Of the Strains Used As Inoculant Treatmentsmentioning

confidence: 63%

“…It is necessary to separately consider colonization and nodulation abilities from symbiotic nitrogen fixation abilities (Checcucci et al, 2017), as rhizobial strains highly competitive for nodule occupancy do not necessarily fix nitrogen efficiently (Westhoek et al, 2017). Thus, elite strains must combine the two aforementioned capabilities, i.e., effectively compete with the native rhizobia for nodule occupancy and effectively provide the plant with fixed nitrogen (Checcucci et al, 2017;Onishchuk et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Competition has also been discussed from two different perspectives by Onishchuk et al (2017): exploitative (indirect), involving more effectively utilizing a common limiting nutrient, or by interference (direct), preventing other cells from growing and surviving in the environment. Regarding exploitative competition, bacterial chemotaxis toward exuded compounds is an important trait for root colonization and plant-driven selection of microorganisms (Bais et al, 2006).…”

Section: Competing Ability Of the Strains Used As Inoculant Treatmentsmentioning

confidence: 99%

“…In terms of interference competition, one of the strategies is the production of antibacterial compounds, such as bacteriocins (Onishchuk et al, 2017). Production of small bacteriocin appears to be a typical character of all fast-growing rhizobia (R. leguminosarum, R. trifolii and R. phaseoli) (Wijffelman et al, 1983).…”

Section: Competing Ability Of the Strains Used As Inoculant Treatmentsmentioning

confidence: 99%

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Formulation of a Highly Effective Inoculant for Common Bean Based on an Autochthonous Elite Strain of Rhizobium leguminosarum bv. phaseoli, and Genomic-Based Insights Into Its Agronomic Performance

Pastor-Bueis¹,

Sánchez-Cañizares²,

James³

et al. 2019

Front. Microbiol.

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Ensifer meliloti denitrification is involved in infection effectiveness and N2O emissions from alfalfa root nodules

et al. 2023

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Purpose Alfalfa is one of the most valuable forage crops in temperate climate zones. Ensifer meliloti, the endosymbiont of alfalfa, contains all the denitrification genes but the capacity of alfalfa root nodules to produce nitrous oxide (N2O) is not known. In this work, N2O emissions as well as the influence of bacteroidal denitrification on nodulation competitiveness and N2O release from alfalfa nodules has been investigated. Methods Medicago sativa cv. Victoria plants were inoculated with E. meliloti 1021, a periplasmic nitrate reductase (Nap) defective mutant, a Nap overexpressing strain and a nitrous oxide reductase defective mutant. Plants were grown in the presence of different nitrate and copper treatments and subjected to flooding during one week before harvesting. N2O production by the nodules was analysed by using gas chromatography. Methyl viologen-dependent nitrate reductase (MV+-NR), nitrite reductase (MV+-NIR) and nitrous oxide reductase (N2OR) enzymatic activities were measured in isolated bacteroids. Results Alfalfa root nodules produce N2O in response to nitrate and flooding. Overexpression of Nap improved nodulation competitiveness and induced N2O emissions from nodules. Copper is required for an effective symbiosis as well as triggered a reduction of N2O production due to the induction of the N2OR and a reduction of NIR activities in the bacteroids. Conclusion Alfalfa root nodules emit N2O. Nap is involved in nodulation competitiveness and in N2O emissions by the nodules. Bacteroidal N2OR and NIR activities are modulated by Cu and may be considered as effective targets for the mitigation strategies of N2O emissions derived from alfalfa crops.

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Nodulation competitiveness of nodule bacteria: Genetic control and adaptive significance: Review

Cited by 18 publications

References 51 publications

Search for Ancestral Features in Genomes of Rhizobium leguminosarum bv. viciae Strains Isolated from the Relict Legume Vavilovia formosa

Search for Ancestral Features in Genomes of Rhizobium leguminosarum bv. viciae Strains Isolated from the Relict Legume Vavilovia formosa

Formulation of a Highly Effective Inoculant for Common Bean Based on an Autochthonous Elite Strain of Rhizobium leguminosarum bv. phaseoli, and Genomic-Based Insights Into Its Agronomic Performance

Ensifer meliloti denitrification is involved in infection effectiveness and N2O emissions from alfalfa root nodules

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