Nodulation and nitrogen fixation by <i>Mimosa</i> spp. in the Cerrado and Caatinga biomes of Brazil

Reis, Fábio Bueno dos; Simon, Marcelo F.; Gross, Eduardo; Boddey, Robert M.; Elliott, Geoffrey N.; Neto, Nicolau Elias; Loureiro, Maria de Fátima; Queiroz, Luciano Paganucci de; Scotti, Maria Rita; Chen, Wen‐Ming; Norén, Agneta; Rubio, Maria C.; Faria, Sérgio Miana de; Bontemps, Cyril; Goi, Silvia Regina; Young, J. Peter W.; Sprent, Janet I.; James, Euan K.

doi:10.1111/j.1469-8137.2010.03267.x

Cited by 157 publications

(130 citation statements)

References 43 publications

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“…The selection of strains, such as those of the genus Burkholderia, which are able to adapt to certain soil conditions, can improve yield under field conditions and reduce the use of N fertilizers and, consequently, agricultural costs (Alves et al, 2016). Reis Jr et al (2010) analyzed the nodulation and biological N 2 fixation of Mimosa species in the neotropical savanna and Caatinga biomes in Brazil. The authors suggest that Burkholderia spp.…”

Section: Discussionmentioning

confidence: 99%

Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant

Castro

Souza

Rufini

et al. 2017

Rev. Bras. Ciênc. Solo

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How to cite: Castro JL, Souza MG, Rufini M, Guimarães AA, Rodrigues TL, Moreira FMS. Diversity and efficiency of rhizobia communities from iron mining areas using cowpea as a trap plant. Rev Bras Cienc Solo. 2017;41:e0160525. https://doi.org/10.1590/18069657rbcs20160525Copyright: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided that the original author and source are credited. Division -Soil Processes and Properties ABSTRACT:Mining is an important economic activity. However, its impact on environment must be accessed, mainly on relevant processes for their sustainability. The objective of this study was to evaluate the diversity and efficiency of symbiotic nitrogen fixing bacterial communities in soils under different types of vegetation in the Quadrilátero Ferrífero: ironstone outcrops, Atlantic Forest, neotropical savanna, and a rehabilitated area revegetated with grass. Suspensions of soil samples collected under each type of vegetation were made in a saline solution to capture rhizobia communities that were then inoculated on cowpea [Vigna unguiculata (L.) Walp.], which was used as a trap plant. The symbiotic efficiency of the communities was evaluated in a greenhouse experiment and the data obtained were correlated to the chemical and physical properties of the soils under each type of vegetation. At the end of the experiment, the bacteria present in the nodules were isolated to evaluate their diversity. The highest numbers of nodules occurred in the treatment inoculated with soil samples from rehabilitated area revegetated with grass and neotropical savanna vegetation, and the lowest numbers were observed in the treatment inoculated with soil samples from ironstone outcrops and Atlantic Forest. In relation to root dry matter, the treatment inoculated with soil samples from Neotropical savanah was superior to those inoculated with soil samples from the other areas; already, in relation to the shoot dry matter, no significant difference among the treatments was observed. The soil properties with the greatest influence on the microbial communities were Al 3+ content, considered as high in the Atlantic Forest and neotropical savanna vegetation, as intermediate in the iron outcrops, and as very low in the rehabilitated area revegetated with grass; organic matter, considered as very high in the ironstone outcrops and neotropical savanna, as high in the Atlantic Forest, and as low in the rehabilitated area revegetated with grass; and the pH, with intermediate acidity level in the rehabilitated area revegetated with grass, high level of acidity in the iron outcrops and neotropical savanna, and very high acidity in the Atlantic Forest. After isolation of the nodules, 380 bacterial strains were obtained and separated into 27 groups by cultural characterization analysis. Genetic diversity was evaluated by the 16S rRNA gene partial sequencing of 156 strains, which identifie...

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Section: Discussionmentioning

confidence: 99%

Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant

Castro

Souza

Rufini

et al. 2017

Rev. Bras. Ciênc. Solo

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“…Plants belonging to the Mimosa genus are the best studied among "Caatinga" native legumes, and advances in the taxonomy of their rhizobia have shown the prevalence of β-rhizobia, mainly Paraburkholderia spp. (formerly Burkholderia) (Reis Júnior et al, 2010;Martins et al, 2015). Regarding Papilionoidae tree legumes, results achieved around the world have indicated the prevalence of α-rhizobia inside their root nodules, although β-rhizobia have also already been observed with lower frequency (Rasolomampianina et al, 2005;Elliott et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil

Menezes

Escobar

Fraiz

et al. 2017

Rev. Bras. Ciênc. Solo

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Legume-rhizobia symbiosis is a cross-kingdom association that results in large amounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic efficiency of eight rhizobial isolates obtained from "mulungu" (Erythrina velutina Willd.) nodules. For 16S rRNA gene sequencing, the genomic DNA was extracted using a commercial kit, amplified with universal primers, and subjected to sequencing reactions. For the isolate ESA 71, PCR amplifications for nodC and nodA genes were attempted. Rhizobial efficiency was assessed by two greenhouse experiments. The first assay was carried out under gnotobiotic conditions, with sterile sand as a substrate; the second experiment was conducted in a non-sterile soil. For both experiments, the inoculation treatments consisted of a single inoculation of each isolate, in addition to a treatment with Bradyrhizobium elkanii BR 5609 as a reference strain. Furthermore, two non-inoculated control treatments, supplied and not supplied with mineral N, were also evaluated. Bacterial identification indicated that both α and β-rhizobia could be found in "mulungu" root nodules. Three isolates where classified within the Rhizobium genus, four bacteria belonged to Bradyrhizobium and one isolate clustered with Burkholderia. Positive amplification of an intragenic fragment of the nodA gene using a primer set to β-rhizobia could be found for ESA 71 (Burkholderia). All bacterial isolates were effective in colonizing "mulungu" roots. In the first experiment, all inoculated treatments and N fertilization increased the N concentration in "mulungu" shoot tissues. For total N in the shoots, the isolates ESA 70, ESA 72, and ESA 75 stood out. In the non-sterile substrate experiment, the isolates ESA 70, ESA 71, ESA 72, and ESA 75, together with the reference strains, induced increases in the shoot N concentration and total accumulation compared to the absolute control. The results indicate that "mulungu" is able to establish associations with efficient α and β-rhizobia in Brazilian semi-arid soils.

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“…These are mainly from the genus Burkholderia, which are the dominant nodule occupants of species of the large sub-tropical genus Mimosa found in the Cerrado and the Caatinga dos Reis et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Novel Burkholderia bacteria isolated from Lebeckia ambigua – A perennial suffrutescent legume of the fynbos

Howieson

Meyer

Vivas-Marfisi

et al. 2013

Soil Biology and Biochemistry

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Nodulation and nitrogen fixation by Mimosa spp. in the Cerrado and Caatinga biomes of Brazil

Cited by 157 publications

References 43 publications

Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant

Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant

Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil

Novel Burkholderia bacteria isolated from Lebeckia ambigua – A perennial suffrutescent legume of the fynbos

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