The effect of climate was observed on fruit quality of four low-chill peach cultivars (Flordaprince, Flordaglo, UFGold, and TropicBeauty). The cultivars were evaluated in three locations (north–central, central, and southwest Florida). Soluble solids content (SSC), titratable acidity (TA), SSC:TA ratio, fruit weight, blush, and fruit development period (FDP) were determined. Longer FDPs were observed at the north–central location than at the southwest location. Fruit development and the expression of quality attributes were affected by location during fruit growth with higher color and SSC and shorter FDP occurring under warmer conditions. Within locations, ‘UFGold’ had the shortest FDP except at the southwest location where its chilling requirement may not have been met. At the central and southwest locations, ‘UFGold’ also tended to have lower TA values and higher SSC;TA ratios than the other cultivars.