Nodavirus-based biological container for targeted delivery system

Jariyapong, Pitchanee

doi:10.3109/21691401.2014.889702

Cited by 6 publications

(5 citation statements)

References 55 publications

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“…The ability of chimeric MrNV VLPs to encapsulate EGFP plasmid DNA at about 40–50% was as expected 22 , 23 . The ultrastructures of these chimeric VLPs during their disassembly and re-assembly process to encapsulate EGFP plasmid (as revealed by TEM negative staining, Supplementary Fig.…”

Section: Resultssupporting

confidence: 69%

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Chimeric virus-like particles (VLPs) designed from shrimp nodavirus (MrNV) capsid protein specifically target EGFR-positive human colorectal cancer cells

Grataitong

Huault

Chotwiwatthanakun

et al. 2021

Sci Rep

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Recombinant MrNV capsid protein has been shown to effectively deliver plasmid DNA and dsRNA into Sf9 insect cells and shrimp tissues. To extend its application to cancer cell-targeting drug delivery, we created three different types of chimeric MrNV virus-like particles (VLPs) (R-MrNV, I-MrNV, and E-MrNV) that have specificity toward the epidermal growth factor receptor (EGFR), a cancer cell biomarker, by incorporating the EGFR-specific GE11 peptide at 3 different locations within the host cell recognition site of the capsid. All three chimeric MrNV-VLPs preserved the ability to form a mulberry-like VLP structure and to encapsulate EGFP DNA plasmid with an efficiency comparable to that previously reported for normal MrNV (N-MrNV). Compared to N-MrNV, the chimeric R-MrNV and E-MrNV carrying the exposed GE-11 peptide showed a significantly enhanced binding and internalization abilities that were specific towards EGFR expression in colorectal cancer cells (SW480). Specific targeting of chimeric MrNV to EGFR was proven by both EGFR silencing with siRNA vector and a competition with excess GE-11 peptide as well as the use of EGFR-negative colorectal cells (SW620) and breast cancer cells (MCF7). We demonstrated here that both chimeric R-MrNV and E-MrNV could be used to encapsulate cargo such as exogenous DNA and deliver it specifically to EGFR-positive cells. Our study presents the potential use of surface-modified VLPs of shrimp virus origin as nanocontainers for targeted cancer drug delivery.

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Section: Resultssupporting

confidence: 69%

“…Principally, MrNV-VLPs could 22 . The controllability of MrNV-VLPs to encapsulate nucleotide-based agents (DNA vector and dsRNA) against the severely infectious virus through the uses of EGTA + DTT (specific calcium chelator and reducing agent, respectively) is also well established both in vitro and in vivo 22 , 23 .…”

Section: Introductionmentioning

confidence: 99%

Chimeric virus-like particles (VLPs) designed from shrimp nodavirus (MrNV) capsid protein specifically target EGFR-positive human colorectal cancer cells

Grataitong

Huault

Chotwiwatthanakun

et al. 2021

Sci Rep

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“…We further modified the test of this VLP’s property to encapsulate a plasmid DNA tagged with the traceable green fluorescent reporter which would allow us to follow VLP targeting in the shrimp tissues. In addition, as the exogenous calcium ions have been proven to be essential for shrimp virus assembly (Jariyapong, 2015), we thus included it into the encapsidation process to facilitate and control VLP disassembly/reassembly. Figure 3A demonstrated the mulberry-like structural feature with the diameter of about 27 nm of the untreated IHHNV-VLPs as reported earlier (Hou et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

Nanocontainer designed from an infectious hypodermal and hematopoietic necrosis virus (IHHNV) has excellent physical stability and ability to deliver shrimp tissues

Kiatmetha

Chotwiwatthanakun

Jariyapong

et al. 2018

PeerJ

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BackgroundA virus-like particle (VLP) is an excellent tool for a compound delivery system due to its simple composition, symmetrical structure and self-assembly. Its surface modification both chemically and genetically is established, leading to the target-specific delivery and improved encapsulation efficiency. However, its physical stabilities against many harsh conditions that guarantee long term storage and oral administration have been much less studied.MethodsIHHNV-VLPs were reconstructed from recombinant IHHNV capsid protein in E. coli. Their physical properties against three strong physical conditions including long term storage (0–30 days) in 4 °C, physical stabilities against broad ranged pH (4–9) and against three types of digestive enzymes were tested. Disassembly and reassembly of VLPs for encapsidating an enhanced green fluorescent protein tagged plasmid DNA (EGFP-VLPs) were controlled by the use of reducing agent (DTT) and calcium specific chelating agent (EGTA). Lastly, delivering ability of EGFP-VLPs was performed in vivo by intramuscular injection and traced the expression of GFP in the shrimp tissues 24 hr post-injection.ResultsUpon its purification, IHHNV-VLPs were able to be kept at 4 °C up to 30 days with only slight degradation. They were very stable in basic condition (pH 8–9) and to a lesser extent in acidic condition (pH 4–6) while they could stand digestions of trypsin and chymotrypsin better than pepsin. As similar with many other non-enveloped viruses, the assembly of IHHNV-VLPs was dependent on both disulfide bridging and calcium ions which allowed us to control disassembly and reassembly of these VLPs to pack EGFP plasmid DNA. IHHNV-VLPs could deliver EGFP plasmids into shrimp muscles and gills as evident by RT-PCR and confocal microscopy demonstrating the expression of GFP in the targeted tissues.DiscussionThere are extensive data in which capsid proteins of the non-enveloped viruses in the form of VLPs are constructed and used as nano-containers for therapeutic compound delivery. However, the bottleneck of its application as an excellent delivery container for oral administration would rely solely on physical stability and interacting ability of VLPs to the host cells. These properties are retained for IHHNV-VLPs reported herein. Thus, IHHNV-VLPs would stand as a good applicable nanocontainer to carry therapeutic agents towards the targeting tissues against ionic and digestive conditions via oral administration in aquaculture field.

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“…Recent reported outbreaks of nodaviruses, diagnostic assays, host immunological responses, vaccines, and virus-like particles (VLPs) are emphasized. To the best of our knowledge, there are only eight review articles related to nodavirus which had been published within the past five years: immunological-based detection of shrimp viruses ( Chaivisuthangkura, Longyant & Sithigorngul, 2014 ); recombinant nodavirus-like particles as delivery system ( Jariyapong, 2015 ); the life cycle of beta-nodaviruses ( Low et al, in press ); viral encephalopathy and retinopathy in aquaculture ( Doan et al, 2017 ); interaction between beta-nodavirus and its host for development of prophylactic measures for viral encephalopathy and retinopathy ( Costa & Thompson, 2016 ); reactive oxygen species-mediated cell death ( Reshi, Su & Hong, 2014 ); mitochondrial disruption and necrotic cell death ( Hong, 2013 ); and immunity to beta-nodavirus infections of marine fish ( Chen, Wang & Chen, 2014 ). Another two review articles published within the past 10 years are about the biology and biomedical applications of Flock House virus ( Venter & Schneemann, 2008 ), and white-tail-disease (WTD) in Macrobrachium rosenbergii ( Bonami & Sri Widada, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Advances in the study of nodavirus

Yong

Yeap

Omar

et al. 2017

PeerJ

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Nodaviruses are small bipartite RNA viruses which belong to the family of Nodaviridae. They are categorized into alpha-nodavirus, which infects insects, and beta-nodavirus, which infects fishes. Another distinct group of nodavirus infects shrimps and prawns, which has been proposed to be categorized as gamma-nodavirus. Our current review focuses mainly on recent studies performed on nodaviruses. Nodavirus can be transmitted vertically and horizontally. Recent outbreaks have been reported in China, Indonesia, Singapore and India, affecting the aquaculture industry. It also decreased mullet stock in the Caspian Sea. Histopathology and transmission electron microscopy (TEM) are used to examine the presence of nodaviruses in infected fishes and prawns. For classification, virus isolation followed by nucleotide sequencing are required. In contrast to partial sequence identification, profiling the whole transcriptome using next generation sequencing (NGS) offers a more comprehensive comparison and characterization of the virus. For rapid diagnosis of nodavirus, assays targeting the viral RNA based on reverse-transcription PCR (RT-PCR) such as microfluidic chips, reverse-transcription loop-mediated isothermal amplification (RT-LAMP) and RT-LAMP coupled with lateral flow dipstick (RT-LAMP-LFD) have been developed. Besides viral RNA detections, diagnosis based on immunological assays such as enzyme-linked immunosorbent assay (ELISA), immunodot and Western blotting have also been reported. In addition, immune responses of fish and prawn are also discussed. Overall, in fish, innate immunity, cellular type I interferon immunity and humoral immunity cooperatively prevent nodavirus infections, whereas prawns and shrimps adopt different immune mechanisms against nodavirus infections, through upregulation of superoxide anion, prophenoloxidase, superoxide dismutase (SOD), crustin, peroxinectin, anti-lipopolysaccharides and heat shock proteins (HSP). Potential vaccines for fishes and prawns based on inactivated viruses, recombinant proteins or DNA, either delivered through injection, oral feeding or immersion, are also discussed in detail. Lastly, a comprehensive review on nodavirus virus-like particles (VLPs) is presented. In recent years, studies on prawn nodavirus are mainly focused on Macrobrachium rosenbergii nodavirus (MrNV). Recombinant MrNV VLPs have been produced in prokaryotic and eukaryotic expression systems. Their roles as a nucleic acid delivery vehicle, a platform for vaccine development, a molecular tool for mechanism study and in solving the structures of MrNV are intensively discussed.

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Nodavirus-based biological container for targeted delivery system

Cited by 6 publications

References 55 publications

Chimeric virus-like particles (VLPs) designed from shrimp nodavirus (MrNV) capsid protein specifically target EGFR-positive human colorectal cancer cells

Chimeric virus-like particles (VLPs) designed from shrimp nodavirus (MrNV) capsid protein specifically target EGFR-positive human colorectal cancer cells

Nanocontainer designed from an infectious hypodermal and hematopoietic necrosis virus (IHHNV) has excellent physical stability and ability to deliver shrimp tissues

Advances in the study of nodavirus

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