Nodal regulates neural tube formation in the Ciona intestinalis embryo

Mita, Kaoru; Fujiwara, Shigeki

doi:10.1007/s00427-007-0168-x

Cited by 43 publications

(46 citation statements)

References 45 publications

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“…We suspected that the behavior of these cells might be controlled by the lateral Nodal signal that distinguishes them from their medial counterparts before the 64-cell stage (Hudson et al, 2007). To test this idea, we performed time-lapse imaging on embryos electroporated with FoxD>Lefty, which inhibits Nodal signaling and causes lateral cells to express medial marker genes (Mita and Fujiwara, 2007).…”

Section: Live Imaging Of Neurulationmentioning

confidence: 99%

“…9A-D). The FoxD enhancer drives expression in A5.1, A5.2, and B5.1 blastomeres starting at the 16-cell stage (Mita and Fujiwara, 2007). To determine whether Nodal signaling is required beyond the early gastrula stage, we expressed Lefty using the later-acting Etr enhancer.…”

Section: Live Imaging Of Neurulationmentioning

confidence: 99%

“…To test whether Nodal signaling was sufficient to induce stacking behavior, we ectopically expressed Nodal in vegetal regions using a FoxD>Nodal fusion gene (see Mita and Fujiwara, 2007). Nuclei were visualized with a Snail>H2B:YFP reporter gene ( Fig.…”

Section: Live Imaging Of Neurulationmentioning

confidence: 99%

“…The modified coding sequence was then amplified using the primers F-TATGCGGCCGCGATGCCTCC-TAAACGTAAGTTAAACC, R-TATGAATTCTTAATCAGGTACAGTG-TCTGGACT and cloned into a vector containing the FoxB enhancer using NotI and EcoRI restriction sites. FoxD>Lefty and FoxD>Nodal (Mita and Fujiwara, 2007) were generously obtained from Shigeki Fujiwara. To construct Etr>Lefty, the Lefty coding sequence was amplified with F-TATGCGGCCGCGATGGCCCGGGCGAA and R-GGATTTCCTTA-CGCGAAATACG, digested with NotI/EcoRI and cloned into a vector containing the Etr enhancer.…”

Section: Molecular Cloningmentioning

confidence: 99%

“…1B;Hudson and Yasuo, 2005;Hudson et al, 2007;Hudson et al, 2015;Imai et al, 2006). Disruption of this signal causes neural tube defects and misexpression of genes known to be involved in neural tube patterning and morphogenesis (Mita and Fujiwara, 2007;Mita et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Nodal and FGF coordinate ascidian neural tube morphogenesis

Navarrete

Levine

2016

Development

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Formation of the vertebrate neural tube represents one of the premier examples of morphogenesis in animal development. Here, we investigate this process in the simple chordate Ciona intestinalis. Previous studies have implicated Nodal and FGF signals in the specification of lateral and ventral neural progenitors. We show that these signals also control the detailed cellular behaviors underlying morphogenesis of the neural tube. Live-imaging experiments show that FGF controls the intercalary movements of ventral neural progenitors, whereas Nodal is essential for the characteristic stacking behavior of lateral cells. Ectopic activation of FGF signaling is sufficient to induce intercalary behaviors in cells that have not received Nodal. In the absence of FGF and Nodal, neural progenitors exhibit a default behavior of sequential cell divisions, and fail to undergo the intercalary and stacking behaviors essential for normal morphogenesis. Thus, cell specification events occurring prior to completion of gastrulation coordinate the morphogenetic movements underlying the organization of the neural tube.

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Section: Live Imaging Of Neurulationmentioning

confidence: 99%

Section: Live Imaging Of Neurulationmentioning

confidence: 99%

Section: Live Imaging Of Neurulationmentioning

confidence: 99%

Section: Molecular Cloningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nodal and FGF coordinate ascidian neural tube morphogenesis

Navarrete

Levine

2016

Development

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Right across the tree of life: The evolution of left–right asymmetry in the Bilateria

Namigai

Kenny

Shimeld

2014

Genesis

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Directional left/right (LR) asymmetries, in which there are consistent, heritable differences in morphology between the left and right sides of bilaterally symmetrical organisms, are found in animals across the Bilateria. For many years, we have lacked evidence for shared mechanisms underlying their development. This led to the supposition that the mechanisms driving establishment of LR asymmetries, and consequently the asymmetries themselves, had evolved separately in the three major Superphyla that constitute the Bilateria. The recent discovery that the transforming growth factor-beta (TGF-B) ligand Nodal plays a role in the regulation of LR asymmetry in both Deuterostomia and Lophotrochozoa has reignited debate in this field, as it suggests that at least this aspect of the development of the LR axis is conserved. In this review, we discuss evidence for shared mechanisms of LR asymmetry establishment across the bilaterian tree of life and consider how these mechanisms might have diverged across the Metazoa over the last 500 million years or so of evolution. As well as the likelihood that Nodal is an ancestral mechanism for regulating LR asymmetry, we reemphasize cytoskeletal architecture as a potential shared mechanism underlying symmetry breaking. However, convergent evolution remains a distinct possibility and study of a wider diversity of species will be needed to distinguish between conserved and lineage-specific mechanisms. genesis 52:458-470,

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Quantitative and in toto imaging in ascidians: Working toward an image‐centric systems biology of chordate morphogenesis

Veeman

Reeves

2014

Genesis

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Developmental biology relies heavily on microscopy to image the finely-controlled cell behaviors that drive embryonic development. Most embryos are large enough that a field of view with the resolution and magnification needed to resolve single cells will not span more than a small region of the embryo. Ascidian embryos, however, are sufficiently small that they can be imaged in toto with fine subcellular detail using conventional microscopes and objectives. Unlike other model organisms with particularly small embryos, ascidians have a chordate embryonic body plan that includes a notochord, hollow dorsal neural tube, heart primordium and numerous other anatomical details conserved with the vertebrates. Here we compare the size and anatomy of ascidian embryos with those of more traditional model organisms, and relate these features to the capabilities of both conventional and exotic imaging methods. We review the emergence of Ciona and related ascidian species as model organisms for a new era of image-based developmental systems biology. We conclude by discussing some important challenges in ascidian imaging and image analysis that remain to be solved.

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Nodal regulates neural tube formation in the Ciona intestinalis embryo

Cited by 43 publications

References 45 publications

Nodal and FGF coordinate ascidian neural tube morphogenesis

Nodal and FGF coordinate ascidian neural tube morphogenesis

Right across the tree of life: The evolution of left–right asymmetry in the Bilateria

Quantitative and in toto imaging in ascidians: Working toward an image‐centric systems biology of chordate morphogenesis

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