NOD/SCID/γcnull mouse: an excellent recipient mouse model for engraftment of human cells

Ito, Mamoru; Hiramatsu, Hidefumi; Kobayashi, Kimio; Suzue, Kazutomo; Kawahata, Mariko; Hioki, Kyoji; Ueyama, Yoshito; Koyanagi, Yoshio; Sugamura, Kazuo; Tsuji, Kohichiro; Heike, Toshio; Nakahata, Tatsutoshi

doi:10.1182/blood-2001-12-0207

Cited by 1,305 publications

(1,198 citation statements)

References 27 publications

(30 reference statements)

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“…At the same time, it is desirable for transplanted cells to have a microenvironment suitable for proliferation as well as tissue organization. This postulated hospitable microenvironment seemed to have little effect on the immunocompetency of the recipients because, contrary to our expectation, NOG mice, which are the most severely immunodeficient among the three mouse strains [19], did not show the most successful development of porcine spermatogenesis.…”

Section: Discussioncontrasting

confidence: 99%

“…NOG mice (NOD/ Shi-SCID, IL-2Rγ c null ) were created at the Central Institute of Experimental Animals (Kawasaki, Japan) by backcrossing γ c null null mice to NOD/Shi-SCID mice, as reported earlier [19]. Four NOG mice, 6 weeks old, Takeshi Watanabe et al…”

Section: Animalsmentioning

confidence: 99%

“…In addition, we hypothesize that immunodeficiency of the host mice influences the success rate of ectopic spermatogenesis of inter-species transplantation, because NOD-NOD/ Shi-SCID, IL-2Rγ c null (NOG), severe combined immunodeficient (SCID) or NOG mice, which are more severely immunocompromised than nude and SCID mice, are more suitable hosts for accepting and sustaining human hematopoietic cells [19]. Therefore, we compare the efficiency of spermatogenesis between cell-injection and tissue grafting methods in three different types of immunodeficient mice (nude, SCID and NOG) to examine the effect of immunological conditions on the de novo formation of seminiferous tubules and spermatogenesis in graft tissues.…”

Section: Introductionmentioning

confidence: 99%

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Ectopic porcine spermatogenesis in murine subcutis: tissue grafting versus cell-injection methods

Watanabe¹,

Hayashi²,

Kita³

et al. 2009

Asian J Androl

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Fragments of testis tissue from immature animals grow and develop spermatogenesis when grafted onto subcutaneous areas of immunodeficient mice. The same results are obtained when dissociated cells from immature testes of rodents are injected into the subcutis of nude mice. Those cells reconstitute seminiferous tubules and facilitate spermatogenesis. We compared these two methods, tissue grafting and cell-injection methods, in terms of the efficiency of spermatogenesis in the backs of three strains of immunodeficient mice, using neonatal porcine testicular tissues and cells as donor material. Nude, severe combined immunodeficient (SCID) and NOD/Shi-SCID, IL-2Rγ c null (NOG) mice were used as recipients. At 10 months after surgery, the transplants were examined histologically. Both grafting and cell-injection methods resulted in porcine spermatogenesis on the backs of recipient mice; the percentage of spermatids present in the transplants was 67% and 22%, respectively. Using the grafting method, all three strains of mice supported the same extent of spermatogenesis. As for the cellinjection method, although SCID mice were the best host for supporting reconstitution and spermatogenesis, any difference from the other strains was not significant. As NOG mice did not show any better results, the severity of immunodeficiency seemed to be irrelevant for supporting xeno-ectopic spermatogenesis. Our results confirmed that tubular reconstitution is applicable to porcine testicular cells. This method as well as the grafting method would be useful for studying spermatogenesis in different kinds of animals.

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Section: Discussioncontrasting

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ectopic porcine spermatogenesis in murine subcutis: tissue grafting versus cell-injection methods

Watanabe¹,

Hayashi²,

Kita³

et al. 2009

Asian J Androl

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“…Superimmunodeficient NOG mice (18) were purchased from Central Institute for Experimental Animals. To generate a mouse model for liver metastasis, Fucci-HCT116 cells (1 Â 10 6 cells) were injected into spleen of male mice (25-27 g; 15-weeks-old; n ¼ 7).…”

Section: Maldi-ims Analyses Of Liver Metastases Of Human Colon Cancermentioning

confidence: 99%

“…In the present study, to investigate coordination between energy metabolism and cell-cycle progression of cancer cells in vivo, we have used a human colorectal cancer cell line HCT116 to provide a liver metastasis model in superimmunodeficient NOD/SCID/IL-2Rg c null (NOG) mice (14)(15)(16)(17)(18). To examine energy metabolism of individual cancer cells in metastatic tumors, we used HCT116 cells labeled with a cell-cycle phase-dependent fluorescent marker system Fucci (19).…”

Section: Introductionmentioning

confidence: 99%

Energy Management by Enhanced Glycolysis in G1-phase in Human Colon Cancer Cells In Vitro and In Vivo

Bao

Mukai

Hishiki

et al. 2013

Molecular Cancer Research

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Activation of aerobic glycolysis in cancer cells is well known as the Warburg effect, although its relation to cellcycle progression remains unknown. In this study, human colon cancer cells were labeled with a cell-cycle phasedependent fluorescent marker Fucci to distinguish cells in G 1 -phase and those in S þ G 2 /M phases. Fucci-labeled cells served as splenic xenograft transplants in super-immunodeficient NOG mice and exhibited multiple metastases in the livers, frozen sections of which were analyzed by semiquantitative microscopic imaging mass spectrometry. Results showed that cells in G 1 -phase exhibited higher concentrations of ATP, NADH, and UDP-N-acetylglucosamine than those in S and G 2 -M phases, suggesting accelerated glycolysis in G 1 -phase cells in vivo. Quantitative determination of metabolites in cells synchronized in S, G 2 -M, and G 1 phases suggested that efflux of lactate was elevated significantly in G 1 -phase. By contrast, ATP production in G 2 -M was highly dependent on mitochondrial respiration, whereas cells in S-phase mostly exhibited an intermediary energy metabolism between G 1 and G 2 -M phases. Isogenic cells carrying a p53-null mutation appeared more active in glycolysis throughout the cell cycle than wild-type cells. Thus, as the cell cycle progressed from G 2 -M to G 1 phases, the dependency of energy production on glycolysis was increased while the mitochondrial energy production was reciprocally decreased.Implications: These results shed light on distinct features of the phase-specific phenotypes of metabolic systems in cancer cells. Mol Cancer Res; 11(9); 973-85. Ó2013 AACR.

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Creation of “Humanized” Mice to Study Human Immunity

2008

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Abstract"Humanized" mice are a promising translational model for studying human hematopoiesis and immunity. Their utility has been enhanced by the development of new stocks of immunodeficient hosts, most notably mouse strains such as NOD-scid IL2rγ null mice that lack the IL-2 receptor common gamma chain. These stocks of mice lack adaptive immune function, display multiple defects in innate immunity, and support heightened levels of human hematolymphoid engraftment. Humanized mice can support studies in many areas of immunology, including autoimmunity, transplantation, infectious diseases, and cancer. These models are particularly valuable in experimentation where there is no appropriate small animal model of the human disease, as in the case of certain viral infections. This unit details the creation of humanized mice by engraftment of immunodeficient mice with hematopoietic stem cells or peripheral blood mononuclear cells, provides methods for evaluating engraftment, and discusses considerations for choosing the appropriate model system to meet specific goals.

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NOD/SCID/γcnull mouse: an excellent recipient mouse model for engraftment of human cells

Cited by 1,305 publications

References 27 publications

Ectopic porcine spermatogenesis in murine subcutis: tissue grafting versus cell-injection methods

Ectopic porcine spermatogenesis in murine subcutis: tissue grafting versus cell-injection methods

Energy Management by Enhanced Glycolysis in G1-phase in Human Colon Cancer Cells In Vitro and In Vivo

Creation of “Humanized” Mice to Study Human Immunity

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