Nociception in chicken embryos, Part II: Embryonal development of electroencephalic neuronal activity<i>in ovo</i>as a prerequisite for nociception

Kollmansperger, Sandra; Anders, Malte; Werner, Julia; Saller, Anna M.; Weiss, Larissa; Suess, Stephanie C.; Reiser, Judith; Schneider, Gerhard; Schusser, Benjamin; Baumgartner, Christine; Fenzl, Thomas

doi:10.1101/2023.04.14.536947

Cited by 5 publications

(3 citation statements)

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“…During Post Pinch, the observed movement changes in ED15 to ED18 embryos were most likely a response to the noxious mechanical stimulus and can therefore be interpreted as nocifensive behavior. The results of our current movement analysis in combination with the corresponding results of the cardiovascular changes 22 and the evaluation of the onset of physiological neuronal signals 23 in chicken embryos during this developmental period provide valuable information that enhances our understanding of the development of nociception and pain perception in chicken.…”

Section: Discussionmentioning

confidence: 98%

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Nociception in chicken embryos, Part III: Analysis of movements before and after application of a noxious stimulus

Suess

Werner

Saller

et al. 2023

Preprint

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Many potentially noxious interventions are performed on chicken embryos in research and in the poultry industry. It is therefore essential and in the interest of animal welfare to be able to precisely define the point at which a chicken embryo is capable of nociception in ovo. The present part III of a comprehensive study examined the movements of developing chicken embryos with the aim of identifying behavioral responses to a noxious stimulus. For this purpose, a noxious mechanical stimulus and a control stimulus were applied in a randomized order. The recorded movements of the embryos were evaluated using the markerless pose estimation software DeepLabCut and manual observations. After the application of the mechanical stimulus, a significant increase in beak movement was identified in 15- to 18-day-old embryos. In younger embryos, no behavioral changes related to the noxious stimulus were observed. The presented results results indicate that noxious mechanical stimuli at the beak base evoke a nocifensive reaction in chicken embryos starting at embryonic day 15.

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Section: Discussionmentioning

confidence: 98%

“…The markerless pose estimation software DeepLabCut (DLC) and manual observations were used to analyze embryonic behavior [19][20][21] . In addition, cardiovascular 22 and electrophysiological 23 parameters were investigated in parts I and II of the comprehensive study.…”

Section: Introductionmentioning

confidence: 99%

Nociception in chicken embryos, Part III: Analysis of movements before and after application of a noxious stimulus

Suess

Werner

Saller

et al. 2023

Preprint

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“…The development of the nervous system begins from EDD 7, while a functional brain is developed by EDD 13. There is less consensus on when the perception of the pain exactly starts; what is known is that nociception starts after the second week ( Kollmansperger et al, 2023 ; Weiss et al, 2023 ). Furthermore, the chick embryo is naturally confined within the shell until it hatches; therefore, it does not have any psychological discomfort from the isolation.…”

Section: Introductionmentioning

confidence: 99%

NEST3D printed bone-mimicking scaffolds: assessment of the effect of geometrical design on stiffness and angiogenic potential

Doyle,

Pannella,

Onofrillo

et al. 2024

Front. Cell Dev. Biol.

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Tissue-engineered implants for bone regeneration require consideration regarding their mineralization and vascularization capacity. Different geometries, such as biomimetic designs and lattices, can influence the mechanical properties and the vascularization capacity of bone-mimicking implants. Negative Embodied Sacrificial Template 3D (NEST3D) printing is a versatile technique across a wide range of materials that enables the production of bone-mimicking scaffolds. In this study, different scaffold motifs (logpile, Voronoi, and trabecular bone) were fabricated via NEST3D printing in polycaprolactone to determine the effect of geometrical design on stiffness (10.44 ± 6.71, 12.61 ± 5.71, and 25.93 ± 4.16 MPa, respectively) and vascularization. The same designs, in a polycaprolactone scaffold only, or when combined with gelatin methacryloyl, were then assessed for their ability to allow the infiltration of blood vessels in a chick chorioallantoic membrane (CAM) assay, a cost-effective and time-efficient in ovo assay to assess vascularization. Our findings showed that gelatin methacrylolyl alone did not allow new chorioallantoic membrane tissue or blood vessels to infiltrate within its structure. However, polycaprolactone on its own or when combined with gelatin methacrylolyl allowed tissue and vessel infiltration in all scaffold designs. The trabecular bone design showed the greatest mineralized matrix production over the three designs tested. This reinforces our hypothesis that both biomaterial choice and scaffold motifs are crucial components for a bone-mimicking scaffold.

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Nociception in Chicken Embryos, Part III: Analysis of Movements before and after Application of a Noxious Stimulus

Süß,

Werner,

Saller

et al. 2023

Animals

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Many potentially noxious interventions are performed on chicken embryos in research and in the poultry industry. It is therefore essential and in the interest of animal welfare to be able to precisely define the point at which a chicken embryo is capable of nociception in ovo. The present part III of a comprehensive study examined the movements of developing chicken embryos with the aim of identifying behavioral responses to a noxious stimulus. For this purpose, a noxious mechanical stimulus and a control stimulus were applied in a randomized order. The recorded movements of the embryos were evaluated using the markerless pose estimation software DeepLabCut and manual observations. After the application of the mechanical stimulus, a significant increase in beak movement was identified in 15- to 18-day-old embryos. In younger embryos, no behavioral changes related to the noxious stimulus were observed. The presented results indicate that noxious mechanical stimuli at the beak base evoke a nocifensive reaction in chicken embryos starting at embryonic day 15.

show abstract

Nociception in chicken embryos, Part II: Embryonal development of electroencephalic neuronal activityin ovoas a prerequisite for nociception

Cited by 5 publications

References 86 publications

Nociception in chicken embryos, Part III: Analysis of movements before and after application of a noxious stimulus

Nociception in chicken embryos, Part III: Analysis of movements before and after application of a noxious stimulus

NEST3D printed bone-mimicking scaffolds: assessment of the effect of geometrical design on stiffness and angiogenic potential

Nociception in Chicken Embryos, Part III: Analysis of Movements before and after Application of a Noxious Stimulus

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