Nocardioides stalactiti sp. nov., isolated from a cave stalactite surface

Abstract

An aerobic, rod-shaped, Gram-stain-positive, actinobacterial strain, designated 1.0914T, was isolated from a stalactite sample collected from a cave located in Guizhou Province, southwest PR China. Based on 16S rRNA gene sequence analysis, strain 1.0914T shared highest similarities values with Nocardioides pelophilus CGMCC 4.7388T (97.7 %), Nocardioides immobilis CCTCC AB 2017083T (97.5 %) and Nocardioides silvaticus CCTCC AB 2018079T (97.3 %) and values lower than 97.0 % to other members of the genus Nocardioides . Phylogenetic trees based on 16S rRNA gene sequences indicated that strain 1.0914T formed an isolated branch with N. pelophilus CGMCC 4.7388T, N. immobilis CCTCC AB 2017083T and N. silvaticus CCTCC AB 2018079T. The polar lipids contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and one unidentified phospholipid in the cellular membrane. The major fatty acids were identified as iso-C16 : 0, C18 : 1 ω9c, C17 : 1 ω8c and C16 : 0. The predominant respiratory quinone was MK-8(H4) and ll-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The genomic DNA G+C content was 71.1 mol%. The orthologous average nucleotide identiy values between N. pelophilus CGMCC 4.7388T, N. immobilis CCTCC AB 2017083T, N. silvaticus CCTCC 2018079T and strain 1.0914T were 82.3, 81.7 and 81.9 % respectively. DNA–DNA hybridization values between N. pelophilus CGMCC 4.7388T, N. immobilis CCTCC AB 2017083T, N. silvaticus CCTCC 2018079T and strain 1.0914T were 25.2, 24.6 and 24.5 % respectively. The phylogenetic, phenotypic and chemotaxonomic data supported the classification of strain 1.0914T as representing a new species of Nocardioides , for which the name Nocardioides stalactiti sp. nov. is proposed. The type strain is 1.0914T (=CCTCC AB 2018266T=KCTC 49243T).

Nocardioides nematodiphilus sp. nov., isolated from rhizosphere of Arabidopsis thaliana

“…The major fatty acids (>10.0 %) were identified as iso-C 16 : 0 , anteiso-C 17 : 0 and iso-C 17 : 0 . The profile of major fatty acids was similar to other members of the genus Nocardioides [2–10]. The fatty acids profiles of strain R-N-C8 T and its related reference strains were given in .…”

“…So far, the genus Nocardioides encompasses 129 validly published species based on the List of Prokaryotic Names with Standing in Nomenclature (https://lpsn.dsmz.de/genus/nocardioides). Members of the genus Nocardioides are characterized as being Gram-stain-positive, rod or coccus-shaped and aerobic with a high DNA G+C content [2][3][4][5][6][7][8][9]. Major chemotaxonomic features of the genus Nocardioides are the presence of MK-8 (H4) as the major respiratory quinone, ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and iso-C 16 : 0 as the major fatty acid.…”

Nocardioides mangrovi sp. nov., a novel endophytic actinobacterium isolated from root of Kandelia candel

“…Among these, the recently reported Nocardioides were separated from a variety of environments, such as Acrostichum aureum, Tagetes patula, soil of rhizosphere, tibetan antelope, dandelion, sewage sludge, cave, etc. [2][3][4][5][6][7]. Members of the genus Nocardioides are characterized as being Gram-positive and aerobic with a high DNA G+C content (68.7-74.9 mol%).…”