Nocardioides pakistanensis sp. nov., isolated from a hot water spring of Tatta Pani in Pakistan

Abstract

A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated as NCCP-1340(T), was isolated from a hot water spring, Tatta Pani, Pakistan. Cells of strain NCCP-1340(T) were observed to be aerobic, rod shaped, catalase and urease positive but H2S production and oxidase negative. Growth was observed at pH 6.0-8.0 (optimum pH 7.0) and at 20-40 °C (optimum 37 °C). The strain could tolerate 0-8 % NaCl (optimum 2 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain NCCP-1340(T) belongs to the genus Nocardioides and is closely related to Nocardioides iriomotensis JCM 17985(T) (96.8 %), Nocardioides daedukensis KCTC 19601(T) (96.6 %), Nocardioides jensenii KCTC 9134(T) (96.1 %) and Nocardioides daejeonensis KCTC 19772(T) (96.1 %). The DNA-DNA relatedness values of strain NCCP-1340(T) with N. iriomotensis JCM 17985(T), N. daedukensis KCTC 19601(T) and N. jensenii KCTC 9134(T) were found to be less than 53 %. The DNA G+C content of strain NCCP-1340(T) was determined to be 71.8 mol %. The affiliation of strain NCCP-1340(T) to the genus Nocardioides was further supported by chemotaxonomic data which showed the presence of MK-8(H4) as major menaquinone system; iso-C16:0, C17:0, C16:0 10-methyl, iso-C15:0 and C 15:0 as major cellular fatty acids; and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and unidentified glycolipids and polar lipids in the polar lipids profile. The cell wall peptidoglycan contained LL-diaminopimelic acid as the diagnostic amino acid. On the basis of physiological and biochemical characteristics and the phylogenetic analyses, strain NCCP-1340(T) can be distinguished from the closely related taxa and thus represents a novel species of the genus Nocardioides, for which the name Nocardioides pakistanensis sp. nov. is proposed with the type strain NCCP-1340(T) (= DSM 29942(T) = JCM 30630(T)).

16S amplicon sequencing of microbial communities in enriched and non-enriched sediments of non-volcanic hot spring with temperature gradients

“…We isolated a MY-TP24 strain as a potential novel candidate species showing close branching with uncultured bacteria. Previously, Amin et al reported a novel species from the Tatta Pani hot springs (Amin et al, 2016). Together, these findings suggest unexplored diversity of novel bacteria in the Tatta Pani hot spring.…”

“…This heated water rises to the surface and mixes with shallow, non-thermal groundwater to form a hot stream (Anees, Shah & Qureshi, 2015;Baioumy et al, 2015). Studies reported thermophilic bacteria from Tatta Pani hot spring used mainly culture-dependent approaches (Amin et al, 2016;Jadoon et al, 2014;Javed et al, 2012).…”

Diversity of culturable thermophilic bacteria from Tata Pani hotspring of Kotli Azad Jammu and Kashmir

“…They reported that B. amyloliquefaciens AK9 can be used in bioconversion of lignocellulosic biomass to fermentable sugar ( Amin et al 2016a , Amin et al 2016b , Zahra et al 2020 ).…”

Identification of Nocardioides marmotae sp. nov. and Nocardioides faecalis sp. nov., two new members of the genus Nocardioides

“…Members within this genus have been isolated from various sources, such as different soil ecosystems (rhizosphere, desert, forest, farmland, etc.) [2][3][4][5], aquatic ecosystems (hot water spring and freshwater lake sediment) [6,7], plants [8][9][10], and animal faeces [11][12][13]. Some species in the genus Nocardioides such as Nocardioides sp.…”