Nocardioides carbamazepini sp. nov., an ibuprofen degrader isolated from a biofilm bacterial community enriched on carbamazepine

Benedek, Tibor; Pápai, Márton; Gharieb, Kholood; Bedics, Anna; Táncsics, Andräs; Tóth, Erika; Hussein, Daood; Maróti, Gergely; Wirth, Roland; Menashe, Ofir; Bóka, Károly; Kriszt, Balázs

doi:10.1016/j.syapm.2022.126339

Cited by 13 publications

(12 citation statements)

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“… * We recently described isolate CBZ_1 T as a new phyletic lineage within the genus Nocardioides to which the name Nocardioides carbamazepini was proposed (Benedek et al 2022 ) …”

Section: Resultsmentioning

confidence: 99%

“…The 16S rRNA gene sequence similarity cutoff value for the demarcation of new bacterial species is 98.7% (Stackebrandt and Ebers 2006 ) or 98.65% (Kim et al 2014 ). Isolate CBZ_1 T has recently been described by the authors as a new phyletic lineage within the genus Nocardioides to which the name Nocardioides carbamazepini was given (Benedek et al 2022 ).…”

Section: Resultsmentioning

confidence: 99%

“…Surprisingly, in our study, the obtained Pseudomonas isolates either proved to be pharmaceutical tolerant or their growth was inhibited by the presence of the tested pharmaceuticals (Online Resource 4). The explanation for this contradictory finding could be that during the tests the applied pharmaceutical concentrations (Benedek et al 2022) were high or Pseudomonas spp. require co-metabolic substrates for the biodegradation of these compounds.…”

Section: Discussionmentioning

confidence: 97%

“…Chen et al ( 2019 ) reported Nocardioides as potential sulfadiazine (antibiotic) degraders. We recently described strain CBZ_1 T as Nocardioides carbamazepini as a new phyletic lineage within the genus Nocardioides capable of IBU biodegradation (Benedek et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…In order to determine more precisely the pharmaceutical biodegradation capacity of those bacterial isolates that showed high activity in the pharmaceuticals amended resazurin test solutions, new microcosm experiments were set up; the concentration of pharmaceuticals was followed using HPLC as described earlier (Benedek et al 2022 ). Briefly, the microcosm experiments were conducted in triplicates in 50 ml of Bushnell-Haas mineral medium amended with one of the investigated pharmaceutical compounds as sole source of carbon and energy (1.5 mg l −1 ).…”

Section: Methodsmentioning

confidence: 99%

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Selective enrichment, identification, and isolation of diclofenac, ibuprofen, and carbamazepine degrading bacteria from a groundwater biofilm

Pápai

Benedek

Táncsics

et al. 2023

Environ Sci Pollut Res

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Diclofenac, ibuprofen, and carbamazepine are three of the most widely detected and most concerning pharmaceutical residues in aquatic ecosystems. The aim of this study was to identify bacteria that may be involved in their degradation from a bacterial biofilm. Selective enrichment cultures in mineral salt solution containing pharmaceutical compounds as sole source of carbon and energy were set up, and population dynamics were monitored using shotgun metagenome sequencing. Bacterial genomes were reconstructed using genome-resolved metagenomics. Thirty bacterial isolates were obtained, identified at species level, and tested regarding pharmaceutical biodegradation at an initial concentration of 1.5 mg l−1. The results indicated that most probably diclofenac biodegrading cultures consisted of members of genera Ferrovibrio, Hydrocarboniphaga, Zavarzinia, and Sphingopyxis, while in ibuprofen biodegradation Nocardioides and Starkeya, and in carbamazepine biodegradation Nocardioides, Pseudonocardia, and Sphingopyxis might be involved. During the enrichments, compared to the initial state the percentage relative abundance of these genera increased up to three orders of magnitude. Except Starkeya, the genomes of these bacteria were reconstructed and annotated. Metabolic analyses of the annotated genomes indicated that these bacteria harbored genes associated with pharmaceutical biodegradation. Stenotrophomonas humi DIC_5 and Rhizobium daejeonense IBU_18 isolates eliminated diclofenac and ibuprofen during the tests in the presence of either glucose (3 g l−1) or in R2A broth. Higher than 90% concentration reduction was observed in the case of both compounds.

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“… * We recently described isolate CBZ_1 T as a new phyletic lineage within the genus Nocardioides to which the name Nocardioides carbamazepini was proposed (Benedek et al 2022 ) …”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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